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N-fixing legumes have a higher demand for phosphorus (P) than legumes grown on mineral N. Medicago truncatula is an important model plant for characterization of effects of P deficiency at the molecular level. Hence, a study was carried out to address the alteration in metabolite levels of M. truncatula grown aeroponically and subjected to 4 weeks of P stress. First, GC-MS-based untargeted metabolomics initially revealed changes in the metabolic profile of nodules, with increased levels of amino acids and sugars and a decline in amounts of organic acids. Subsequently, LC-MS\/MS was used to quantify these compounds including phosphorylated metabolites in the whole plant. Our results showed a drastic reduction in levels of organic acids and phosphorylated compounds in \u2013P leaves, with a moderate reduction in \u2013P roots and nodules. Additionally, sugars and amino acids were elevated in the whole plant under P deprivation. These findings provide evidence that N fixation in M. truncatula is mediated through a N feedback mechanism that in parallel is related to carbon and P metabolism.<\/jats:p>","DOI":"10.1093\/jxb\/erab559","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T12:14:18Z","timestamp":1640952858000},"page":"2093-2111","source":"Crossref","is-referenced-by-count":20,"title":["Metabolite shift in <i>Medicago truncatula<\/i> occurs in phosphorus deprivation"],"prefix":"10.1093","volume":"73","author":[{"given":"Dhiraj","family":"Dokwal","sequence":"first","affiliation":[{"name":"BioDiscovery Institute, University of North Texas , Denton, TX 76203 , USA"},{"name":"Department of Biological Sciences, University of North Texas , Denton, TX 76203 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Cocuron","sequence":"additional","affiliation":[{"name":"BioDiscovery Institute, University of North Texas , Denton, TX 76203 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Alonso","sequence":"additional","affiliation":[{"name":"BioDiscovery Institute, University of North Texas , Denton, TX 76203 , USA"},{"name":"Department of Biological Sciences, University of North Texas , Denton, TX 76203 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5401-426X","authenticated-orcid":false,"given":"Rebecca","family":"Dickstein","sequence":"additional","affiliation":[{"name":"BioDiscovery Institute, University of North Texas , Denton, TX 76203 , USA"},{"name":"Department of Biological Sciences, University of North Texas , Denton, TX 76203 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,31]]},"reference":[{"key":"2022123101014536500_CIT0001","first-page":"1289","article-title":"Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L.).","volume":"51","author":"Almeida","year":"2000","journal-title":"Journal of Experimental Botany"},{"key":"2022123101014536500_CIT0002","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/BF02668879","article-title":"Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium\u2013legume symbiosis.","volume":"8","author":"Barker","year":"1990","journal-title":"Plant Molecular Biology Reporter"},{"key":"2022123101014536500_CIT0003","article-title":"Growing M. truncatula: choice of substrates and growth conditions.","volume-title":"The Medicago truncatula handbook","author":"Barker","year":"2006"},{"key":"2022123101014536500_CIT0004","doi-asserted-by":"crossref","first-page":"e1007304","DOI":"10.1371\/journal.pgen.1007304","article-title":"Natural allelic variation of the AZI1 gene controls root growth under zinc-limiting condition.","volume":"14","author":"Bouain","year":"2018","journal-title":"PLoS Genetics"},{"key":"2022123101014536500_CIT0005","doi-asserted-by":"crossref","first-page":"6035","DOI":"10.1093\/jxb\/eru341","article-title":"RNA-seq transcriptome profiling reveals that Medicago truncatula nodules acclimate N2 fixation before emerging P deficiency reaches the nodules.","volume":"65","author":"Cabeza","year":"2014","journal-title":"Journal of Experimental Botany"},{"key":"2022123101014536500_CIT0006","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1104\/pp.17.01624","article-title":"The impacts of phosphorus deficiency on the photosynthetic electron transport chain.","volume":"177","author":"Carstensen","year":"2018","journal-title":"Plant Physiology"},{"key":"2022123101014536500_CIT0007","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3390\/metabo11040234","article-title":"Targeted metabolic profiles of the leaves and xylem sap of two sugarcane genotypes infected with the vascular bacterial pathogen Leifsonia xyli subsp. 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