{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T01:46:58Z","timestamp":1768009618895,"version":"3.49.0"},"reference-count":71,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fondo Europeo de Desarrollo Regional","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,13]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer tomato plants highly resistant to PSTVd and developed several S. lycopersicum lines expressing an artificial microRNA (amiRNA) against PSTVd (amiR-PSTVd). Infectivity assays revealed that amiR-PSTVd-expressing lines were not resistant but instead hypersusceptible to the viroid. A combination of phenotypic, molecular, and metabolic analyses of amiRNA-expressing lines non-inoculated with the viroid revealed that amiR-PSTVd was accidentally silencing the tomato STEROL GLYCOSYLTRANSFERASE 1 (SlSGT1) gene, which caused late developmental and reproductive defects such as leaf epinasty, dwarfism, or reduced fruit size. Importantly, two independent transgenic tomato lines each expressing a different amiRNA specifically designed to target SlSGT1 were also hypersusceptible to PSTVd, thus demonstrating that down-regulation of SlSGT1 was responsible for the viroid-hypersusceptibility phenotype. Our results highlight the role of sterol glycosyltransferases in proper plant development and indicate that the imbalance of sterol glycosylation levels favors viroid infection, most likely by facilitating viroid movement.<\/jats:p>","DOI":"10.1093\/jxb\/erac361","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T15:32:50Z","timestamp":1663342370000},"page":"1564-1578","source":"Crossref","is-referenced-by-count":9,"title":["Down-regulation of tomato<i>STEROL GLYCOSYLTRANSFERASE 1<\/i>perturbs plant development and facilitates viroid infection"],"prefix":"10.1093","volume":"74","author":[{"given":"Adriana E","family":"Cisneros","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2013Universitat Polit\u00e8cnica de Val\u00e8ncia) , 46022 Valencia , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Purificaci\u00f3n","family":"Lis\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2013Universitat Polit\u00e8cnica de Val\u00e8ncia) , 46022 Valencia , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Campos","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2013Universitat Polit\u00e8cnica de Val\u00e8ncia) , 46022 Valencia , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan Manel","family":"L\u00f3pez-Tubau","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CSIC-IRTA-IAB-UB) , Bellaterra, Barcelona , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Altabella","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CSIC-IRTA-IAB-UB) , Bellaterra, Barcelona , Spain"},{"name":"Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona , 08028 Barcelona , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Centre for Research in Agricultural Genomics (CSIC-IRTA-IAB-UB) , Bellaterra, Barcelona , Spain"},{"name":"Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, University of Barcelona , 08028 Barcelona , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9-Antonio","family":"Dar\u00f2s","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2013Universitat Polit\u00e8cnica de Val\u00e8ncia) , 46022 Valencia , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5628-6632","authenticated-orcid":false,"given":"Alberto","family":"Carbonell","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2013Universitat Polit\u00e8cnica de Val\u00e8ncia) , 46022 Valencia , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"17949","DOI":"10.1038\/srep17949","article-title":"RNAi mediated inhibition of viroid infection in transgenic plants expressing viroid-specific small RNAs derived from various functional domains","volume":"5","author":"Adkar-Purushothama","year":"2015","journal-title":"Scientific Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"e1570","DOI":"10.1002\/wrna.1570","article-title":"Current overview on viroid\u2013host interactions","volume":"11","author":"Adkar-Purushothama","year":"2020","journal-title":"WIREs RNA"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00299-021-02799-x","article-title":"Phytosterol metabolism in plant positive-strand RNA virus replication","volume":"41","author":"Altabella","year":"2022","journal-title":"Plant Cell Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2014a Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1099\/0022-1317-76-7-1781","article-title":"The expression of antisense and ribozyme genes targeting citrus exocortis viroid in transgenic plants","volume":"76","author":"Atkins","year":"1995","journal-title":"Journal of General Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"e0144","DOI":"10.1199\/tab.0144","article-title":"Cytochromes P450.","volume":"2011","author":"Bak","year":"2011","journal-title":"The Arabidopsis Book"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1146\/annurev.arplant.55.031903.141616","article-title":"Biosynthesis and accumulation of sterols","volume":"55","author":"Benveniste","year":"2004","journal-title":"Annual Review of Plant Biology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1126\/science.6197756","article-title":"A replication cycle for viroids and other small infectious RNA\u2019s","volume":"223","author":"Branch","year":"1984","journal-title":"Science"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"e0159875","DOI":"10.1371\/journal.pone.0159875","article-title":"Functional characterization of CYP94-genes and identification of a novel jasmonate catabolite in flowers","volume":"11","author":"Bruckhoff","year":"2016","journal-title":"PLoS One"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/mpp.12529","article-title":"Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection","volume":"18","author":"Carbonell","year":"2017","journal-title":"Molecular Plant Pathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1111\/tpj.12835","article-title":"Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors","volume":"82","author":"Carbonell","year":"2015","journal-title":"The Plant Journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1093\/nar\/gkq1051","article-title":"Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA","volume":"39","author":"Carbonell","year":"2011","journal-title":"Nucleic Acids Research"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.virol.2007.09.031","article-title":"Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families","volume":"371","author":"Carbonell","year":"2008","journal-title":"Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1104\/pp.113.234989","article-title":"New generation of artificial microRNA and synthetic trans-acting small interfering RNA vectors for efficient gene silencing in Arabidopsis","volume":"165","author":"Carbonell","year":"2014","journal-title":"Plant Physiology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.3389\/fpls.2019.01162","article-title":"Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea","volume":"10","author":"Castillo","year":"2019","journal-title":"Frontiers in Plant Science"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"105181","DOI":"10.1016\/j.envexpbot.2022.105181","article-title":"Tomato STEROL GLYCOSYLTRANSFERASE 1 unveils a major role of steryl glycosydes in plant and fruit development","volume":"206","author":"Ch\u00e1vez","year":"2023","journal-title":"Environmental and Experimental Botany"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1105\/tpc.113.118935","article-title":"The role of Arabidopsis ABCG9 and ABCG31 ATP binding cassette transporters in pollen fitness and the deposition of steryl glycosides on the pollen coat","volume":"26","author":"Choi","year":"2014","journal-title":"The Plant Cell"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"8649","DOI":"10.1093\/nar\/gkz679","article-title":"Citrus exocortis viroid causes ribosomal stress in tomato plants","volume":"47","author":"Cottilli","year":"2019","journal-title":"Nucleic Acids Research"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"634","DOI":"10.3390\/v7020634","article-title":"Engineering viroid resistance","volume":"7","author":"Dalakouras","year":"2015","journal-title":"Viruses"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/978-3-319-32919-2_13","article-title":"Viroids: small noncoding infectious RNAs with the remarkable ability of autonomous replication.","volume-title":"Current Research Topics in Plant Virology","author":"Dar\u00f2s","year":"2016"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1104\/pp.109.140582","article-title":"Mutations in UDP-glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds","volume":"151","author":"DeBolt","year":"2009","journal-title":"Plant Physiology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1046\/j.1365-313X.1997.12040931.x","article-title":"Cell-to-cell movement of potato spindle tuber viroid","volume":"12","author":"Ding","year":"1997","journal-title":"The Plant Journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00122-002-0928-y","article-title":"The ploidy level of transgenic plants in Agrobacterium-mediated transformation of tomato cotyledons (Lycopersicon esculentum Mill.) is genotype and procedure dependent","volume":"106","author":"Ellul","year":"2003","journal-title":"Theoretical and Applied Genetics"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/978-1-60327-005-2_4","article-title":"miRNA target prediction in plants","volume":"592","author":"Fahlgren","year":"2010","journal-title":"Methods in Molecular Biology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/bioinformatics\/btv534","article-title":"P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design","volume":"32","author":"Fahlgren","year":"2016","journal-title":"Bioinformatics"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.phyto.43.040204.140243","article-title":"Viroids and viroid-host interactions","volume":"43","author":"Flores","year":"2005","journal-title":"Annual Review of Phytopathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.virusres.2015.02.027","article-title":"Viroids, the simplest RNA replicons: how they manipulate their hosts for being propagated and how their hosts react for containing the infection","volume":"209","author":"Flores","year":"2015","journal-title":"Virus Research"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.coviro.2017.07.003","article-title":"Engineering resistance against viroids","volume":"26","author":"Flores","year":"2017","journal-title":"Current Opinion in Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1146\/annurev.arplant.54.031902.134921","article-title":"Biosynthesis and metabolism of brassinosteroids","volume":"54","author":"Fujioka","year":"2003","journal-title":"Annual Review of Plant Biology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: a graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1104\/pp.108.120808","article-title":"Viroid-induced symptoms in Nicotiana benthamiana plants are dependent on RDR6 activity","volume":"148","author":"Gomez","year":"2008","journal-title":"Plant Physiology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0885-5765(87)90008-7","article-title":"Induction of pathogenesis-related proteins in tomato by citrus exocortis viroid, silver ion and ethephon","volume":"31","author":"Granell","year":"1987","journal-title":"Physiological and Molecular Plant Pathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1105\/tpc.114.135731","article-title":"Specific membrane lipid composition is important for plasmodesmata function in Arabidopsis","volume":"27","author":"Grison","year":"2015","journal-title":"The Plant Cell"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"5810","DOI":"10.1074\/jbc.M114.598805","article-title":"Differential effect of plant lipids on membrane organization: specificities of phytosphingolipids and phytosterols","volume":"290","author":"Grosjean","year":"2015","journal-title":"Journal of Biological Chemistry"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/273203a0","article-title":"Nucleotide sequence and secondary structure of potato spindle tuber viroid","volume":"273","author":"Gross","year":"1978","journal-title":"Nature"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1006\/viro.1995.1230","article-title":"Interdependence of pathogenicity and replicability with potato spindle tuber viroid","volume":"209","author":"Gruner","year":"1995","journal-title":"Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nature Methods"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1002\/pmic.201200286","article-title":"A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato","volume":"13","author":"Lis\u00f3n","year":"2013","journal-title":"Proteomics"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"6234","DOI":"10.1093\/nar\/gkaa343","article-title":"Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs","volume":"48","author":"L\u00f3pez-Dolz","year":"2020","journal-title":"Nucleic Acids Research"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1515\/bchm3.1994.375.11.765","article-title":"Inhibition of viroid infection by antisense RNA expression in transgenic plants","volume":"375","author":"Matou\u0161ek","year":"1994","journal-title":"Biological Chemistry Hoppe-Seyler"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"11933","DOI":"10.1128\/JVI.01404-14","article-title":"Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo","volume":"88","author":"Minoia","year":"2014","journal-title":"Journal of Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.pmpp.2016.11.003","article-title":"Overexpression of Withania somnifera SGTL1 gene resists the interaction of fungus Alternaria brassicicola in Arabidopsis thaliana","volume":"97","author":"Mishra","year":"2017","journal-title":"Physiological and Molecular Plant Pathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1007\/s00299-021-02666-9","article-title":"Overexpression of WssgtL3.1 gene from Withania somnifera confers salt stress tolerance in Arabidopsis","volume":"40","author":"Mishra","year":"2021","journal-title":"Plant Cell Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.cell.2008.02.033","article-title":"Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation","volume":"133","author":"Montgomery","year":"2008","journal-title":"Cell"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/S0163-7827(02)00006-1","article-title":"Phytosterols, phytostanols, and their conjugates in foods: structural diversity, quantitative analysis, and health-promoting uses","volume":"41","author":"Moreau","year":"2002","journal-title":"Progress in Lipid Research"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1146\/annurev-virology-091919-092331","article-title":"Advances in viroid-host interactions","volume":"8","author":"Navarro","year":"2021","journal-title":"Annual Review of Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"3725","DOI":"10.3390\/ijms22073725","article-title":"Degradome analysis of tomato and Nicotiana benthamiana plants infected with potato spindle tuber viroid","volume":"22","author":"Navarro","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1365-313X.2011.04529.x","article-title":"A P450-centric view of plant evolution","volume":"66","author":"Nelson","year":"2011","journal-title":"The Plant Journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1104\/pp.106.079475","article-title":"CYP707A1 and CYP707A2, which encode abscisic acid 8\u02b9-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis","volume":"141","author":"Okamoto","year":"2006","journal-title":"Plant Physiology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/B978-0-12-801498-1.00010-3","article-title":"Changes in the host proteome and transcriptome induced by viroid infection.","volume-title":"Viroids and Satellites","author":"Owens","year":"2017"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/B978-0-12-801498-1.00008-5","article-title":"Viroid movement.","volume-title":"Viroids and Satellites","author":"Pall\u00e1s","year":"2017"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s00425-014-2046-x","article-title":"WsSGTL1 gene from Withania somnifera, modulates glycosylation profile, antioxidant system and confers biotic and salt stress tolerance in transgenic tobacco","volume":"239","author":"Pandey","year":"2014","journal-title":"Planta"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"5714","DOI":"10.1038\/s41598-017-05925-6","article-title":"Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots","volume":"7","author":"Pook","year":"2017","journal-title":"Scientific Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"984","DOI":"10.3389\/fpls.2017.00984","article-title":"Tomato UDP-glucose sterol glycosyltransferases: a family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme","volume":"8","author":"Ramirez-Estrada","year":"2017","journal-title":"Frontiers in Plant Science"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.1096\/fj.08-111070","article-title":"Depletion of phytosterols from the plant plasma membrane provides evidence for disruption of lipid rafts","volume":"22","author":"Roche","year":"2008","journal-title":"The FASEB Journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1099\/vir.0.81356-0","article-title":"Variants of Peach latent mosaic viroid inducing peach calico: uneven distribution in infected plants and requirements of the insertion containing the pathogenicity determinant","volume":"87","author":"Rodio","year":"2006","journal-title":"Journal of General Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00299-015-1879-5","article-title":"Ectopic overexpression of WsSGTL1, a sterol glucosyltransferase gene in Withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses","volume":"35","author":"Saema","year":"2016","journal-title":"Plant Cell Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1038\/nbt1197-1290","article-title":"Transgenic potato expressing a double-stranded RNA-specific ribonuclease is resistant to potato spindle tuber viroid","volume":"15","author":"Sano","year":"1997","journal-title":"Nature Biotechnology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1364-3703.2009.00546.x","article-title":"RNAi-mediated resistance to Potato spindle tuber viroid in transgenic tomato expressing a viroid hairpin RNA construct","volume":"10","author":"Schwind","year":"2009","journal-title":"Molecular Plant Pathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/0042-6822(76)90500-6","article-title":"Exocortis viroid: cytopathic effects at the plasma membrane in association with pathogenic RNA","volume":"69","author":"Semancik","year":"1976","journal-title":"Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/JVI.02003-09","article-title":"Inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants","volume":"84","author":"Sharma","year":"2010","journal-title":"Journal of Virology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"25562","DOI":"10.1038\/srep25562","article-title":"Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera","volume":"6","author":"Singh","year":"2016","journal-title":"Scientific Reports"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/tpj.15115","article-title":"Ligand\u2013receptor interactions in plant hormone signaling","volume":"105","author":"Takeuchi","year":"2021","journal-title":"The Plant Journal"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s10327-013-0475-1","article-title":"Threats to Japanese agriculture from newly emerged plant viruses and viroids","volume":"80","author":"Tsuda","year":"2014","journal-title":"Journal of General Plant Pathology"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3390\/plants9050582","article-title":"Ethylene is involved in symptom development and ribosomal stress of tomato plants upon citrus exocortis viroid infection","volume":"9","author":"V\u00e1zquez Prol","year":"2020","journal-title":"Plants"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"6189","DOI":"10.3390\/ijms22126189","article-title":"Symptom severity, infection progression and plant responses in Solanum plants caused by three pospiviroids vary with the inoculation procedure","volume":"22","author":"V\u00e1zquez Prol","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"4861","DOI":"10.1073\/pnas.94.10.4861","article-title":"Ribozyme-mediated high resistance against potato spindle tuber viroid in transgenic potatoes","volume":"94","author":"Yang","year":"1997","journal-title":"Proceedings of the National Academy of Sciences, USA"},{"key":"2023032710564845500_","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1242\/dev.060590","article-title":"Brassinosteroid signalling","volume":"140","author":"Zhu","year":"2013","journal-title":"Development"}],"container-title":["Journal of Experimental Botany"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jxb\/advance-article-pdf\/doi\/10.1093\/jxb\/erac361\/48597631\/erac361.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jxb\/article-pdf\/74\/5\/1564\/49647924\/erac361.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jxb\/article-pdf\/74\/5\/1564\/49647924\/erac361.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,26]],"date-time":"2023-11-26T13:48:11Z","timestamp":1701006491000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jxb\/article\/74\/5\/1564\/6702022"}},"subtitle":[],"editor":[{"given":"Sonia","family":"Osorio","sequence":"additional","affiliation":[{"name":"University of Malaga , Spain"}],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,9,16]]},"references-count":71,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,9,16]]},"published-print":{"date-parts":[[2023,3,13]]}},"URL":"https:\/\/doi.org\/10.1093\/jxb\/erac361","relation":{},"ISSN":["0022-0957","1460-2431"],"issn-type":[{"value":"0022-0957","type":"print"},{"value":"1460-2431","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,3,13]]},"published":{"date-parts":[[2022,9,16]]}}}