{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T07:02:07Z","timestamp":1779260527485,"version":"3.51.4"},"reference-count":82,"publisher":"Oxford University Press (OUP)","issue":"18","license":[{"start":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T00:00:00Z","timestamp":1688256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Marie Sk\u0142odowska Curie Post-Doctoral Fellowship","award":["655043"],"award-info":[{"award-number":["655043"]}]},{"name":"UKRI Biotechnology and Biological Sciences Research Council","award":["BB\/N001079\/1"],"award-info":[{"award-number":["BB\/N001079\/1"]}]},{"name":"UKRI Biotechnology and Biological Sciences Research Council","award":["BB\/P012523\/1"],"award-info":[{"award-number":["BB\/P012523\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The mineral micronutrients zinc (Zn) and iron (Fe) are essential for plant growth and human nutrition, but interactions between the homeostatic networks of these two elements are not fully understood. Here we show that loss of function of BTSL1 and BTSL2, which encode partially redundant E3 ubiquitin ligases that negatively regulate Fe uptake, confers tolerance to Zn excess in Arabidopsis thaliana. Double btsl1 btsl2 mutant seedlings grown on high Zn medium accumulated similar amounts of Zn in roots and shoots to the wild type, but suppressed the accumulation of excess Fe in roots. RNA-sequencing analysis showed that roots of mutant seedlings had relatively higher expression of genes involved in Fe uptake (IRT1, FRO2, and NAS) and in Zn storage (MTP3 and ZIF1). Surprisingly, mutant shoots did not show the transcriptional Fe deficiency response which is normally induced by Zn excess. Split-root experiments suggested that within roots the BTSL proteins act locally and downstream of systemic Fe deficiency signals. Together, our data show that constitutive low-level induction of the Fe deficiency response protects btsl1 btsl2 mutants from Zn toxicity. We propose that BTSL protein function is disadvantageous in situations of external Zn and Fe imbalances, and formulate a general model for Zn\u2013Fe interactions in plants.<\/jats:p>","DOI":"10.1093\/jxb\/erad243","type":"journal-article","created":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T23:17:36Z","timestamp":1688339856000},"page":"5767-5782","source":"Crossref","is-referenced-by-count":19,"title":["BRUTUS-LIKE (BTSL) E3 ligase-mediated fine-tuning of Fe regulation negatively affects Zn tolerance of Arabidopsis"],"prefix":"10.1093","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5777-4969","authenticated-orcid":false,"given":"Camilla","family":"Stanton","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Metabolism, John Innes Centre , Norwich NR4 7UH , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7967-5136","authenticated-orcid":false,"given":"Jorge","family":"Rodr\u00edguez-Celma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Metabolism, John Innes Centre , Norwich NR4 7UH , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7870-4508","authenticated-orcid":false,"given":"Ute","family":"Kr\u00e4mer","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Ruhr University Bochum , D-44801 Bochum , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1452-9808","authenticated-orcid":false,"given":"Dale","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Metabolism, John Innes Centre , Norwich NR4 7UH , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4738-1990","authenticated-orcid":false,"given":"Janneke","family":"Balk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Metabolism, John Innes Centre , Norwich NR4 7UH , UK"},{"name":"School of Biological Sciences, University of East Anglia , Norwich NR4 7TJ , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,7,2]]},"reference":[{"key":"2023092913534823000_CIT0001","author":"Andrew","year":"2010"},{"key":"2023092913534823000_CIT0002","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1111\/j.1365-313X.2006.02746.x","article-title":"The Arabidopsis metal tolerance protein AtMTP3 maintains metal homeostasis by mediating Zn exclusion from the shoot under Fe deficiency and Zn oversupply","volume":"46","author":"Arrivault","year":"2006","journal-title":"The Plant 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