{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T01:33:30Z","timestamp":1788917610760,"version":"build-2803163510"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"20","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Photosynthetic Systems Program, Basic Energy Sciences, U.S. Department of Energy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rubisco activase (RCA) facilitates the removal of inhibitors from the Rubisco active site and is essential for plant growth at atmospheric CO2. Here we report the phenotypes of gene-edited RCA mutants in the C4 grass Setaria viridis, which has two forms of RCA, RCA\u03b1 and RCA\u03b2, encoded by separate genes. In Setaria, only the \u03b2 form accumulates at normal temperatures, with the \u03b1 form being induced following exposure to heat. Inactivating the RCA\u03b2 gene (\u03b2KO) abolished accumulation of RCA at 25\u2005\u00b0C and significantly reduced photosynthesis. These plants required elevated CO2 for survival. Inactivating the RCA\u03b1 gene (\u03b1KO) did not affect RCA\u03b2 accumulation, photosynthesis, or growth. Neither mutant exhibited altered Rubisco content or activation when grown at 25\u2005\u00b0C and elevated CO2. When \u03b2KO plants were transferred from elevated to ambient CO2 and 40\u2005\u00b0C, RCA\u03b1 induction supported growth for approximately 7\u2005d, although plants were smaller with fewer tillers compared with WT and \u03b1KO plants. The ability of a small amount of RCA\u03b1 to support growth in ambient CO2 at 40\u2005\u00b0C is consistent with our results showing that its in vitro ATPase activity is more thermotolerant than RCA\u03b2 and suggests that RCA\u03b1 may help C4 plants cope with certain heat stress conditions.<\/jats:p>","DOI":"10.1093\/jxb\/eraf289","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T07:53:32Z","timestamp":1750924412000},"page":"5930-5945","source":"Crossref","is-referenced-by-count":2,"title":["The \u03b1 form of Rubisco activase supports photosynthesis during heat stress in the absence of the \u03b2 form in\n                    <i>Setaria viridis<\/i>"],"prefix":"10.1093","volume":"76","author":[{"given":"Amber M","family":"Hotto","sequence":"first","affiliation":[{"name":"Boyce Thompson Institute , 533 Tower Rd., Ithaca, NY 14853 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