{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:41:09Z","timestamp":1787625669740,"version":"build-2736575974"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000197"],"award-info":[{"award-number":["32000197"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870265"],"award-info":[{"award-number":["31870265"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chloroplast iron\u2013sulfur (Fe\u2013S) clusters, essential cofactors for Fe\u2013S proteins, are assembled by the sulfur utilization factor (SUF) BC2D complex, but regulation of their biogenesis remains unclear. We show that during leaf senescence, SUFB transcription rapidly increases while SUFC\/SUFD transcripts and the abundance of all three SUF proteins remain unchanged. Leaf necrosis with green color occurs solely when SUFB RNA interference (RNAi) is induced, and the decrease of SUFs is obviously faster in SUFB-RNAi than SUFC-RNAi lines. Furthermore, SUFB exhibits a higher turnover rate than SUFC\/SUFD, which accelerates during senescence. Overexpressing SUFB elevates SUFC\/SUFD\/SUFBC2D levels, revealing that SUFB stabilizes SUFC\/SUFD via SUFBC2D complex formation, a function SUFC lacks. Under iron deficiency, SUFB transcript level declines, whereas SUFC\/SUFD transcript levels remain unchanged. Nevertheless, abundance of SUFs decreases, suggesting that the reduced amount of SUFBC2D is mediated by the down-regulation of SUFB transcription. We subsequently found that SUFs accumulated in a caseinolytic protease (CLP)-impaired mutant and exhibited resistance to degradation when cytoplasmic translation was inhibited by cycloheximide, revealing that their turnover is mediated by CLP. Moreover, SUFB, SUFC, and SUFD physically interact with CLPS1, a substrate adaptor of the CLP complex. Collectively, SUFB expression and CLP coordinately regulate Fe\u2013S biogenesis by controlling SUFBC2D abundance, providing critical insights into plant adaption to leaf senescence and iron deficiency.<\/jats:p>","DOI":"10.1093\/jxb\/eraf553","type":"journal-article","created":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T12:48:14Z","timestamp":1766666894000},"page":"1631-1646","source":"Crossref","is-referenced-by-count":2,"title":["Dynamic regulation of the chloroplast SUFBC2D complex by\n                    <i>SUFB<\/i>\n                    expression and CLP protease in Arabidopsis"],"prefix":"10.1093","volume":"77","author":[{"given":"Yuting","family":"Cheng","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University , Yangzhou 225009 ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University , Yangzhou 225009 ,","place":["China"]},{"name":"College of Bioscience and Biotechnology, Yangzhou University , Yangzhou 225009 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University , Yangzhou 225009 ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University , Yangzhou 225009 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Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University , Yangzhou 225009 ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University , Yangzhou 225009 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8288-3155","authenticated-orcid":false,"given":"Xueyun","family":"Hu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University , Yangzhou 225009 ,","place":["China"]},{"name":"Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Joint 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