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Here, we investigated its role in the acclimation of Chlamydomonas reinhardtii to varying CO2 availability. We show that phosphoribulokinase can interact with CP12 in conditions where the Calvin\u2013Benson\u2013Bassham cycle is active. Compared to the wild type, at high CO2 \u00a0C. reinhardtii \u0394CP12 mutants have less phosphoribulokinase and ribulose-1,5-bisphosphate (RuBP), indicating that the regeneration of RuBP is regulated, in part, by CP12. Chlamydomonas reinhardtii has a CO2-concentrating mechanism that increases the supply of CO2 to Rubisco and involves, among other features, the condensation of Rubisco within the pyrenoid via its interaction with a scaffold protein named Essential Pyrenoid Component 1 (EPYC1). In the \u0394CP12 mutants, the expected relocation of Rubisco towards the pyrenoid was not observed upon transition from high to very low CO2, in contrast to wild type cells. The \u0394CP12 mutants are therefore a unique example where the induction of the CO2-concentrating mechanism at very low CO2 is not accompanied by Rubisco relocation. Taken together, our results suggest that CP12 contributes to the coordination between RuBP regeneration, Rubisco location, and CO2 acquisition.<\/jats:p>","DOI":"10.1093\/jxb\/erag050","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:43:54Z","timestamp":1769604234000},"page":"3036-3052","source":"Crossref","is-referenced-by-count":1,"title":["Impact of CP12 deletion on inorganic carbon acquisition and Rubisco partitioning in\n                    <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0917-3074","authenticated-orcid":false,"given":"Cassy","family":"G\u00e9rard","sequence":"first","affiliation":[{"name":"Aix Marseille Univ, CNRS, BIP, UMR 7281, IMM, FR 3479, 31 Chemin J. Aiguier , Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-9013","authenticated-orcid":false,"given":"R\u00e9gine","family":"Lebrun","sequence":"additional","affiliation":[{"name":"Plateforme Prot\u00e9omique, Marseille Prot\u00e9omique (MaP), IMM FR 3479 , 31 Chemin Joseph Aiguier, Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Verthuy","sequence":"additional","affiliation":[{"name":"Plateforme Prot\u00e9omique, Marseille Prot\u00e9omique (MaP), IMM FR 3479 , 31 Chemin Joseph Aiguier, Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1768-1212","authenticated-orcid":false,"given":"Hugo","family":"Le Guenno","sequence":"additional","affiliation":[{"name":"Microscopy Core Facility, Institut de Microbiologie de la M\u00e9diterran\u00e9e (FR 3479), CNRS, Aix Marseille Univ , Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7115-4566","authenticated-orcid":false,"given":"Artemis","family":"Kosta","sequence":"additional","affiliation":[{"name":"Microscopy Core Facility, Institut de Microbiologie de la M\u00e9diterran\u00e9e (FR 3479), CNRS, Aix Marseille Univ , Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Byrne","sequence":"additional","affiliation":[{"name":"Biomass & Protein Engineering Platform, Institut de Microbiologie de la M\u00e9diterran\u00e9e (FR 3479), CNRS, Aix Marseille Univ , Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, BIP, UMR 7281, IMM, FR 3479, 31 Chemin J. Aiguier , Marseille 13402 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Gu\u00e9rard","sequence":"additional","affiliation":[{"name":"Plateforme M\u00e9tabolisme-M\u00e9tabolome, Institute of Plant Sciences Paris-Saclay IPS2, CNRS, INRAE, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cit\u00e9, Universit\u00e9 Paris-Saclay , B\u00e2timent 360, Avenue des sciences, Gif sur Yvette 91192 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Suk","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Research Institute for Natural Sciences, Hanyang University , Seoul 04763 ,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2190-2391","authenticated-orcid":false,"given":"Achille","family":"Marchand","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, CNRS, BIP, UMR 7281, IMM, FR 3479, 31 Chemin J. 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