{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T08:08:00Z","timestamp":1785312480703,"version":"3.55.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T00:00:00Z","timestamp":1775347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32325036"],"award-info":[{"award-number":["32325036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20452"],"award-info":[{"award-number":["U22A20452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32401538"],"award-info":[{"award-number":["32401538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gansu Science and Technology Major Project","award":["24RCKB001"],"award-info":[{"award-number":["24RCKB001"]}]},{"name":"Gansu Science and Technology Major Project","award":["24ZD13NA016"],"award-info":[{"award-number":["24ZD13NA016"]}]},{"name":"Gansu Science and Technology Major Project","award":["22ZD6FA052"],"award-info":[{"award-number":["22ZD6FA052"]}]},{"name":"Gansu Science and Technology Major Project","award":["22ZD6NA007"],"award-info":[{"award-number":["22ZD6NA007"]}]},{"name":"Gansu Science and Technology Major Project","award":["25JRRA737"],"award-info":[{"award-number":["25JRRA737"]}]},{"name":"Gansu Science and Technology Major Project","award":["26JRRA263"],"award-info":[{"award-number":["26JRRA263"]}]},{"name":"Lanzhou City\u2019s High Education Talent Project","award":["563225111"],"award-info":[{"award-number":["563225111"]}]},{"name":"Gansu Province","award":["836012"],"award-info":[{"award-number":["836012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Melatonin (N-acetyl-5-methoxytryptamine) is a conserved indoleamine that regulates diverse physiological processes, including stomatal movement in angiosperms. However, whether this regulation is conserved across different plant lineages remains uncertain. Here, we examined the stomatal responses to exogenous melatonin in 17 representative plant species: seven angiosperms, two gymnosperms, seven ferns, and one lycophyte. In seed plants, melatonin induced dose-dependent stomatal closure, accompanied by the accumulation of reactive oxygen species (ROS), nitric oxide (NO), and cytosolic Ca2+; this response was abolished by ROS and NO scavengers or synthesis inhibitors. In contrast, ferns and the lycophyte exhibited melatonin-induced ROS production but showed no NO or Ca2+ accumulation or stomatal closure. Melatonin application attenuated or completely disrupted circadian-driven stomatal closure under constant light in seed plants. In contrast, rhythmic stomatal closure was not observed under constant light in ferns or lycophytes, with the exception of one semi-aquatic species, Marsilea quadrifolia, which exhibited a rhythmic increase in stomatal conductance that was not influenced by exogenous melatonin treatment. These results indicate that the signaling pathway for melatonin-induced stomatal movement is present in seed plants but absent in ferns and lycophytes, suggesting that variation in melatonin sensitivity may be related to lineage-specific differences in nighttime stomatal behavior.<\/jats:p>","DOI":"10.1093\/jxb\/erag146","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:55:05Z","timestamp":1773838505000},"page":"4668-4678","source":"Crossref","is-referenced-by-count":0,"title":["Lineage-specific response to melatonin in stomatal regulation across vascular plants"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5086-884X","authenticated-orcid":false,"given":"Xu-Dong","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]},{"name":"Department of Plant Sciences, University of Cambridge , Cambridge CB2 3EA ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Li","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Northwest Normal University , Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Lu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Yuan","family":"Zeng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]},{"name":"College of Life Sciences, Northwest Normal University , Lanzhou 730070 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia-Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ru","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Xian","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2227-2800","authenticated-orcid":false,"given":"Xiang-Wen","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University , Lanzhou 730000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,5]]},"reference":[{"key":"2026072903370381200_erag146-B1","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1104\/pp.15.01965","article-title":"NO-mediated [Ca2+]cyt increases depend on ADP-ribosyl cyclase activity in Arabidopsis","volume":"171","author":"Abdul-Awal","year":"2016","journal-title":"Plant Physiology"},{"key":"2026072903370381200_erag146-B2","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.tplants.2014.07.006","article-title":"Melatonin: plant growth regulator and\/or biostimulator during stress?","volume":"19","author":"Arnao","year":"2014","journal-title":"Trends in Plant Science"},{"key":"2026072903370381200_erag146-B3","doi-asserted-by":"crossref","first-page":"152","DOI":"10.32794\/11250036","article-title":"Melatonin and reactive oxygen and nitrogen species: a model for the plant redox network","volume":"2","author":"Arnao","year":"2019","journal-title":"Melatonin Research"},{"key":"2026072903370381200_erag146-B4","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1093\/plphys\/kiad036","article-title":"Light, rather than circadian rhythm, regulates gas exchange in ferns and lycophytes","volume":"191","author":"Aros-Mualin","year":"2023","journal-title":"Plant Physiology"},{"key":"2026072903370381200_erag146-B5","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1126\/science.1197985","article-title":"Passive origins of stomatal control in vascular 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lineages","volume":"186","author":"Gong","year":"2021","journal-title":"Plant Physiology"},{"key":"2026072903370381200_erag146-B9","doi-asserted-by":"crossref","first-page":"114302","DOI":"10.1016\/j.indcrop.2021.114302","article-title":"Effects of single and combined exogenous application of abscisic acid and melatonin on cotton carbohydrate metabolism and yield under drought stress","volume":"176","author":"Hu","year":"2022","journal-title":"Industrial Crops and Products"},{"key":"2026072903370381200_erag146-B10","doi-asserted-by":"crossref","first-page":"677","DOI":"10.3389\/fpls.2019.00677","article-title":"Exogenous melatonin alleviates oxidative damages and protects photosystem II in maize seedlings under drought stress","volume":"10","author":"Huang","year":"2019","journal-title":"Frontiers in Plant Science"},{"key":"2026072903370381200_erag146-B11","doi-asserted-by":"crossref","first-page":"e12379","DOI":"10.1111\/jpi.12379","article-title":"Melatonin is required for H2O2- and NO-mediated defense signaling through MAPKKK3 and OXI1 in Arabidopsis thaliana","volume":"62","author":"Lee","year":"2017","journal-title":"Journal of Pineal Research"},{"key":"2026072903370381200_erag146-B12","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1093\/jxb\/eru476","article-title":"Melatonin mediates the regulation of ABA metabolism, free-radical scavenging, and stomatal behaviour in two Malus species under drought stress","volume":"66","author":"Li","year":"2015","journal-title":"Journal of Experimental Botany"},{"key":"2026072903370381200_erag146-B13","doi-asserted-by":"crossref","first-page":"e12640","DOI":"10.1111\/jpi.12640","article-title":"Daily rhythms of phytomelatonin signaling modulate diurnal stomatal closure via regulating reactive oxygen species dynamics in Arabidopsis","volume":"68","author":"Li","year":"2020","journal-title":"Journal of Pineal 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Melatonin as a regulator of stomatal immunity in plants","volume":"188","author":"Moreno","year":"2022","journal-title":"Plant Physiology"},{"key":"2026072903370381200_erag146-B21","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1186\/s12870-024-05132-2","article-title":"A meta-analysis of photosynthetic efficiency and stress mitigation by melatonin in enhancing wheat tolerance","volume":"24","author":"Muhammad","year":"2024","journal-title":"BMC Plant Biology"},{"key":"2026072903370381200_erag146-B22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.32794\/mr11250003","article-title":"A computer-assisted systematic search for melatonin derivatives with high potential as antioxidants","volume":"1","author":"Reina","year":"2018","journal-title":"Melatonin Research"},{"key":"2026072903370381200_erag146-B23","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.plaphy.2016.01.018","article-title":"Melatonin-induced CBF\/DREB1s are essential for diurnal change of disease resistance and CCA1 expression in Arabidopsis","volume":"100","author":"Shi","year":"2016","journal-title":"Plant Physiology and Biochemistry"},{"key":"2026072903370381200_erag146-B24","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/jipb.12993","article-title":"Melatonin: a master regulator of plant development and stress responses","volume":"63","author":"Sun","year":"2021","journal-title":"Journal of Integrative Plant Biology"},{"key":"2026072903370381200_erag146-B25","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/jpi.12026","article-title":"Mitochondria and chloroplasts as the original sites of melatonin synthesis: a hypothesis related to melatonin\u2019s primary function and evolution in eukaryotes","volume":"54","author":"Tan","year":"2013","journal-title":"Journal of Pineal Research"},{"key":"2026072903370381200_erag146-B26","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.1093\/jxb\/eraa235","article-title":"An evolutionary view of melatonin synthesis and metabolism related to its biological functions in plants","volume":"71","author":"Tan","year":"2020","journal-title":"Journal of Experimental Botany"},{"key":"2026072903370381200_erag146-B27","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.3390\/plants14131952","article-title":"Phylogenetic and structural insights into melatonin receptors in plants: case study in Capsicum chinense Jacq","volume":"14","author":"Toledo-Casti\u00f1eira","year":"2025","journal-title":"Plants"},{"key":"2026072903370381200_erag146-B28","doi-asserted-by":"crossref","first-page":"153917","DOI":"10.1016\/j.jplph.2023.153917","article-title":"Nitric oxide acts downstream of reactive oxygen species in phytomelatonin receptor 1 (PMTR1)-mediated stomatal closure in Arabidopsis","volume":"282","author":"Wang","year":"2023","journal-title":"Journal of Plant 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,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,4,5]]},"references-count":39,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,4,5]]},"published-print":{"date-parts":[[2026,7,29]]}},"URL":"https:\/\/doi.org\/10.1093\/jxb\/erag146","relation":{},"ISSN":["0022-0957","1460-2431"],"issn-type":[{"value":"0022-0957","type":"print"},{"value":"1460-2431","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,7,29]]},"published":{"date-parts":[[2026,4,5]]}}}