{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T14:13:21Z","timestamp":1778854401086,"version":"3.51.4"},"reference-count":107,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National Institute of Bioethanol Science and Technology\u2014INCT of Bioethanol","award":["FAPESP 2014\/50884-5"],"award-info":[{"award-number":["FAPESP 2014\/50884-5"]}]},{"name":"National Institute of Bioethanol Science and Technology\u2014INCT of Bioethanol","award":["CNPq 465319\/2014-9"],"award-info":[{"award-number":["CNPq 465319\/2014-9"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["FAPESP 2014\/50279-4"],"award-info":[{"award-number":["FAPESP 2014\/50279-4"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["2020\/15230-5"],"award-info":[{"award-number":["2020\/15230-5"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["FAPESP 2022\/00441-6"],"award-info":[{"award-number":["FAPESP 2022\/00441-6"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["CAPES 88882.377113\/2019-1"],"award-info":[{"award-number":["CAPES 88882.377113\/2019-1"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["FAPESP 2021\/08334-1"],"award-info":[{"award-number":["FAPESP 2021\/08334-1"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["FAPESP 2019\/13936-0"],"award-info":[{"award-number":["FAPESP 2019\/13936-0"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["CNPq 142090\/2018\u20132"],"award-info":[{"award-number":["CNPq 142090\/2018\u20132"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["RCGI 371055"],"award-info":[{"award-number":["RCGI 371055"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["161174\/2021-3"],"award-info":[{"award-number":["161174\/2021-3"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["FAPESP 2011\/06930-4"],"award-info":[{"award-number":["FAPESP 2011\/06930-4"]}]},{"name":"Research Center of Greenhouse Gas Innovation","award":["2015\/13479-8"],"award-info":[{"award-number":["2015\/13479-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sugarcane is a major feedstock for first-generation ethanol production, but the efficient conversion of its lignocellulosic residues into second-generation biofuels remains limited by the resilience of its cell wall. Here, we suggest sugarcane root aerenchyma as a relevant biological model to study in vivo mechanisms of cell wall breakdown. By performing spatial transcriptomic profiling across four root segments representing different stages of aerenchyma development, we identified &amp;gt;32 000 differentially expressed transcripts, including many genes that encode cell wall-modifying proteins. Co-expression network analysis and functional annotation revealed stage-specific expression of genes encoding glycoside hydrolases, glycosyltransferases, and expansins, aligning with the gradual degradation of wall components. Among these, an \u03b1-L-arabinofuranosidase gene (ScASD1) was chosen for biotechnological validation due to its strong transcriptional induction. Heterologous expression in Pichia pastoris and saccharification assays with sugarcane bagasse showed that ScASD1 significantly improves reducing sugar release when used with a commercial enzyme cocktail. Our results support using sugarcane aerenchyma as a platform to discover plant-derived enzymes, and establish ScASD1 as a promising candidate for enhancing hydrolytic efficiency in second-generation ethanol production. This approach aligns with sustainable bioenergy goals and offers a scalable method to lower costs and promote circularity in sugarcane-based biorefineries.<\/jats:p>","DOI":"10.1093\/jxb\/erag174","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T11:37:49Z","timestamp":1775907469000},"source":"Crossref","is-referenced-by-count":0,"title":["Aerenchyma in sugarcane roots: a toolbox for tailoring biomass for bioenergy"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9289-4328","authenticated-orcid":false,"given":"Bruno V","family":"Navarro","sequence":"first","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4691-6167","authenticated-orcid":false,"given":"D\u00e9bora","family":"Pagliuso","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2048-6664","authenticated-orcid":false,"given":"Mauro de M","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8416-3733","authenticated-orcid":false,"given":"Adriana","family":"Grandis","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7182-6364","authenticated-orcid":false,"given":"Maria J\u00falia Pasian","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9622-7020","authenticated-orcid":false,"given":"Lauana P","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1728-0876","authenticated-orcid":false,"given":"Grayce H","family":"Romim","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5431-7578","authenticated-orcid":false,"given":"Alexandre Junio Borges","family":"Araujo","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9340-7955","authenticated-orcid":false,"given":"Eveline Q P","family":"Tavares","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1757-3625","authenticated-orcid":false,"given":"Amanda R","family":"Piovezani","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Physiological Ecology, Department of Botany, Institute of Biosciences, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5455-8136","authenticated-orcid":false,"given":"Marcos 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