{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:52:50Z","timestamp":1791298370933,"version":"4.1.0"},"reference-count":68,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["22-31712S"],"award-info":[{"award-number":["22-31712S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education, Youth, and Sports of the Czech Republic"},{"name":"TowArds Next GENeration Crops","award":["CZ.02.01.01\/00\/22_008\/0004581"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004581"]}]},{"name":"Programme Johannes Amos Comenius"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quantitative pollen viability analysis is a critical but labor-intensive step in plant reproductive biology. Existing deep-learning Segment Anything Models (SAM) fail to reliably segment viable pollen in Alexander-stained anthers. To address this, we fine-tuned an existing Cellpose-SAM model for pollen segmentation. We integrated it into PAT (Pollen Analysis Tool), a cross-platform desktop application. PAT features instance segmentation with interactive quality control, an in-app model retraining module, and publication-ready statistical outputs. We deployed PAT in an EMS suppressor screen of semi-sterile Arabidopsis mutants carrying the hypomorphic smg7-6 allele, which contains a T-DNA insertion in the C-terminal region of SUPPRESSOR WITH MORPHOGENETIC EFFECTS ON GENITALIA 7 (SMG7), enabling efficient candidate prioritization for whole-genome sequencing and mapping of the candidate mutation. This screen led to the identification of capd2-2, a point mutation in the non-SMC condensin I subunit D2 (CAP-D2) that rescues the smg7-6 meiotic phenotype. Notably, mutation in condensin II subunits (CAP-D3 and CAP-H2) does not confer rescue. Further characterization suggests that the capd2-2 allele is hypomorphic, showing no defects in vegetative growth, chromocenter compaction, or transposable element silencing. Collectively, we demonstrate that accessible AI tools have the potential to bridge gaps in plant phenotyping and accelerate the pace of biological discovery.<\/jats:p>","DOI":"10.1093\/jxb\/erag436","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:21:20Z","timestamp":1788517280000},"source":"Crossref","is-referenced-by-count":0,"title":["PAT: an image analysis tool for automated scoring of pollen in Alexander-stained anthers"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3578-6592","authenticated-orcid":false,"given":"Darya","family":"Volkava","sequence":"first","affiliation":[{"name":"Central European Institute of Technology, Masaryk University , Brno 625 00 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6124-0118","authenticated-orcid":false,"given":"Karel","family":"Riha","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Masaryk University , Brno 625 00 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5182-6377","authenticated-orcid":false,"given":"Vivek Kumar","family":"Raxwal","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Masaryk University , Brno 625 00 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"2026100610013699400_erag436-B1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3109\/10520296909063335","article-title":"Differential staining of aborted and nonaborted pollen","volume":"44","author":"Alexander","year":"1969","journal-title":"Stain Technology"},{"key":"2026100610013699400_erag436-B2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/s41592-024-02580-4","article-title":"Segment anything for 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