{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:37:50Z","timestamp":1792708670415,"version":"4.5.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Short dormancy period of sweetpotato (Ipomoea batatas) storage roots causes significant economic losses. To unravel the molecular mechanism of sweetpotato sprouting, a comparative transcriptome was performed using storage roots incubated under non-sprout (15 \u00b0C) and sprout favouring conditions (25 \u00b0C). Our analysis revealed that 7404 genes were differentially expressed between non-sprouting and sprouted storage roots incubated at 25 \u00b0C. Further, we observed that gibberellin (GA) biosynthesis gene IbGA3OXIDASE and abscisic acid (ABA) catabolism gene ABA 8'-HYDROXYLASE were upregulated by &amp;gt; 10-fold and &amp;gt; 40-fold, respectively, at the onset of sprouting. Metabolite profiling showed increased GA and cytokinin levels, but reduced ABA content in the sprout initials. Based on conserved expression patterns of phytohormone-related genes during sprouting in potato tubers and sweetpotato storage roots, two genes that were downregulated during sweetpotato sprouting - IbPYL4 (ABA receptor) and IbGA2OXIDASE (GA catabolic enzyme) - were independently overexpressed in potato. Transgenic potato plants overexpressing IbPYL4 or IbGA2OXIDASE exhibited low percentages of tuber sprouting and reduced sprout length. Furthermore, knockdown of the ABA 8'-HYDROXYLASE gene (ABA catabolism) significantly reduced sprout length in sweetpotato storage roots compared to wild-type plants. Overall, our study suggests that selective candidate genes (ABA 8'-HYDROXYLASE, IbPYL4, IbGA3OXIDASE, and IbGA2OXIDASE) could be targeted for developing sweetpotato genotypes with enhanced shelf-life.<\/jats:p>","DOI":"10.1093\/jxb\/erag536","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:18:29Z","timestamp":1792408709000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome, metabolite profiling and functional genomics provide molecular insights into sweetpotato sprouting"],"prefix":"10.1093","author":[{"given":"Aruna B","family":"Patil","sequence":"first","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshay B","family":"Vyawahare","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4009-5924","authenticated-orcid":false,"given":"Vitthal T","family":"Barvkar","sequence":"additional","affiliation":[{"name":"Department of Botany, Savitribai Phule Pune University , Pune 411007, Maharashtra ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita S","family":"Gawade","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjit S","family":"Barbole","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]},{"name":"Department of Biochemistry, College of Agriculture, Food and Natural Resources, University of Missouri , Columbia, MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh S","family":"Joshi","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9079-1226","authenticated-orcid":false,"given":"Narendra Y","family":"Kadoo","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0762-3767","authenticated-orcid":false,"given":"Kirtikumar R","family":"Kondhare","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology, Biochemical Sciences Division, CSIR-National Chemical Laboratory , Pune 411008, Maharashtra ,","place":["India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad 201002 ,","place":["India"]},{"name":"Department of Biosciences and Technology, Dr. Vishwanath Karad MIT World Peace University , Pune 411038, Maharashtra ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,22]]},"container-title":["Journal of Experimental Botany"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jxb\/advance-article-pdf\/doi\/10.1093\/jxb\/erag536\/72372708\/erag536.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jxb\/advance-article-pdf\/doi\/10.1093\/jxb\/erag536\/72372708\/erag536.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:56:28Z","timestamp":1792706188000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jxb\/advance-article\/doi\/10.1093\/jxb\/erag536\/8979762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/jxb\/erag536","relation":{},"ISSN":["0022-0957","1460-2431"],"issn-type":[{"value":"0022-0957","type":"print"},{"value":"1460-2431","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"article-number":"erag536"}}