{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T19:14:58Z","timestamp":1768590898334,"version":"3.49.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,6,13]],"date-time":"2020-06-13T00:00:00Z","timestamp":1592006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>A 77 year old female was admitted with a subdural hematoma requiring 1 unit of apheresis platelets. She was a study subject in the 1960s and was found to be Rhnull, along with another individual who previously served as a directed donor for her.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Serologic testing performed by the immunohematology reference laboratory (IRL) confirmed that the patient was Rhnull and expressed anti-Rh29 antibodies. While searching for red blood cells (RBCs) for possible transfusion, it was discovered that the individual from the original study had recently donated an autologous unit.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The IRL discovered that the donor\u2019s antigen typing was r\u2019r\u2019. Testing had been performed using a molecular human erythrocyte antigen BeadChip (HBC). Due to the discrepancy between current and historical testing results, a donor segment was thawed and by tube testing confirmed to be Rhnull. A limitation of HBC is that many null phenotypes will be missed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This case demonstrated that Rhnull evaluation of the donor required both serological and molecular methods.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmaa009","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T20:23:35Z","timestamp":1584217415000},"page":"e1-e4","source":"Crossref","is-referenced-by-count":2,"title":["A Rare Cohort of Two Rhnull Individuals"],"prefix":"10.1093","volume":"52","author":[{"given":"Richard R","family":"Gammon","sequence":"first","affiliation":[{"name":"Immunohematology Reference Laboratory-Cypress Creek, OneBlood, Inc., Ft. Lauderdale, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Delk","sequence":"additional","affiliation":[{"name":"Scientific Medical and Technical Administration, OneBlood, Inc., Orlando, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Houtz","sequence":"additional","affiliation":[{"name":"Jupiter, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Miami Cancer Institute, Miami, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Benitez","sequence":"additional","affiliation":[{"name":"Immunohematology Reference Laboratory-Cypress Creek, OneBlood, Inc., Ft. Lauderdale, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,6,13]]},"reference":[{"key":"2021010415100384600_CIT0001","first-page":"389","article-title":"Rhnull and pregnancy complicated by maternal anti-\u201ctotal Rh.\u201d I. Anti-Rh29","volume":"7","author":"Haber","year":"1967","journal-title":"Transfusion."},{"key":"2021010415100384600_CIT0002","first-page":"389","article-title":"Rhnull and pregnancy complicated by maternal anti-\u201ctotal Rh\u201d II. Additional evidence of multiple anomalies associated with the Rh-29m (rhm) variety of Rhnull red cells","volume":"7","author":"Bar-Shany","year":"1967","journal-title":"Transfusion."},{"issue":"3","key":"2021010415100384600_CIT0003","first-page":"182","article-title":"Two siblings with Rhnull disease","volume":"23","author":"Seidl","year":"1972","journal-title":"Vox Sang."},{"issue":"3","key":"2021010415100384600_CIT0004","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1046\/j.1537-2995.1978.18378205143.x","article-title":"Simple method of improving the quality of platelet concentrates and the importance of production control","volume":"18","author":"Bers\u00e9us","year":"1978","journal-title":"Transfusion."},{"key":"2021010415100384600_CIT0005","article-title":"Whole blood and apheresis collections for blood components intended for transfusion.","volume-title":"Technical Manual","author":"Wagner","year":"2017","edition":"19"},{"key":"2021010415100384600_CIT0006","volume-title":"PreciseType HEA Molecular BeadChip test. Package insert 190-20210-EN Rev D","year":"2018"},{"key":"2021010415100384600_CIT0007","article-title":"Rh system.","volume-title":"Technical Manual","author":"Denomme","year":"2017"},{"issue":"3","key":"2021010415100384600_CIT0008","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1182\/blood.V78.3.551.551","article-title":"Molecular biology of the Rh antigens","volume":"78","author":"Agre","year":"1991","journal-title":"Blood."},{"issue":"2","key":"2021010415100384600_CIT0009","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0950-3536(05)80152-0","article-title":"Biochemical aspects of the blood group Rh (rhesus) antigens","volume":"6","author":"Anstee","year":"1993","journal-title":"Baillieres Clin Haematol."},{"issue":"5","key":"2021010415100384600_CIT0010","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1182\/blood.V63.5.1046.1046","article-title":"Red cell membrane and cation deficiency in Rhnull syndrome","volume":"63","author":"Ballas","year":"1984","journal-title":"Blood."},{"issue":"3","key":"2021010415100384600_CIT0011","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1002\/ajh.2830240306","article-title":"Hematological aspect of Rh deficiency syndrome: a case report and a review of the literature","volume":"24","author":"Nash","year":"1987","journal-title":"Am J Hematol."},{"issue":"3","key":"2021010415100384600_CIT0012","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1042\/bj2210931","article-title":"Rhnull human erythrocytes have an abnormal membrane phospholipid organization","volume":"221","author":"Kuypers","year":"1984","journal-title":"Biochem J."},{"issue":"3","key":"2021010415100384600_CIT0013","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1182\/blood-2003-04-1331","article-title":"Protein 4.2 is critical to CD47-membrane skeleton attachment in human red cells","volume":"103","author":"Dahl","year":"2004","journal-title":"Blood."},{"issue":"28","key":"2021010415100384600_CIT0014","doi-asserted-by":"crossref","first-page":"25526","DOI":"10.1074\/jbc.M302816200","article-title":"Rh-RhAG\/ankyrin-R, a new interaction site between the membrane bilayer and the red cell skeleton, is impaired by Rh(null)-associated mutation","volume":"278","author":"Nicolas","year":"2003","journal-title":"J Biol Chem."},{"issue":"2","key":"2021010415100384600_CIT0015","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1182\/blood.V95.2.375","article-title":"The Rh blood group system: a review","volume":"95","author":"Avent","year":"2000","journal-title":"Blood."},{"key":"2021010415100384600_CIT0016","volume-title":"AABB Standards for Blood Banks and Transfusion Services","author":"Ooley","year":"2017","edition":"31"},{"key":"2021010415100384600_CIT0017","author":"US Department of Health and Human Services"}],"container-title":["Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/labmed\/article-pdf\/52\/1\/e1\/35388888\/lmaa009.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/labmed\/article-pdf\/52\/1\/e1\/35388888\/lmaa009.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,4]],"date-time":"2021-01-04T20:13:03Z","timestamp":1609791183000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/labmed\/article\/52\/1\/e1\/5857001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,13]]},"references-count":17,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,6,13]]},"published-print":{"date-parts":[[2021,1,4]]}},"URL":"https:\/\/doi.org\/10.1093\/labmed\/lmaa009","relation":{},"ISSN":["0007-5027","1943-7730"],"issn-type":[{"value":"0007-5027","type":"print"},{"value":"1943-7730","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,1,1]]},"published":{"date-parts":[[2020,6,13]]}}}