{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:14:01Z","timestamp":1790208841253,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T00:00:00Z","timestamp":1592179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Currently, more than 45 mathematical formulas based on simple red blood cell (RBC) parameters have been proposed for differentiating between iron deficiency and thalassemia in microcytic anemia, of which 20 are relatively new and have not been thoroughly independently verified. The study goal was to verify these 20 new formulas and to identify which RBC parameters have a decisive impact on the performance of those formulas.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A database containing laboratory and diagnostic data from 2788 subject individuals with microcytic anemia was used for assessing performance by receiver operating characteristic (ROC) analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The new Index26 had excellent performance, equivalent to the Green and King, Jayabose, and Janel formulas previously identified in the literature. The discriminant power of nearly all newer formulas was lower in our study than that claimed by the original authors. We discovered that a well-performing formula requires mean cell volume (MCV), RBC distribution width (RDW), and RBC measurements, whereas hemoglobin measurements appeared not to be essential.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Only the new Index26 performed at a level comparable to the very strongest established formulas. All other new formulas had lower performance than was claimed in the original publications, underscoring that independent verification of new formulas is indispensable.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmaa030","type":"journal-article","created":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T11:08:01Z","timestamp":1587812881000},"page":"628-634","source":"Crossref","is-referenced-by-count":19,"title":["Verification of 20 Mathematical Formulas for Discriminating Between Iron Deficiency Anemia and Thalassemia Trait in Microcytic Anemia"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1009-4568","authenticated-orcid":false,"given":"Johannes J M L","family":"Hoffmann","sequence":"first","affiliation":[{"name":"H3L Consult, Nuenen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elo\u00edsa","family":"Urrechaga","sequence":"additional","affiliation":[{"name":"CORE Laboratory, Hospital Galdakao\u2014Usansolo, Galdakao, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,6,15]]},"reference":[{"issue":"8","key":"2020110202111180900_CIT0001","first-page":"704","article-title":"Inherited haemoglobin disorders: an increasing global health problem","volume":"79","author":"Weatherall","year":"2001","journal-title":"Bull World Health Organ."},{"issue":"1","key":"2020110202111180900_CIT0002","doi-asserted-by":"crossref","first-page":"e2017018","DOI":"10.4084\/mjhid.2017.018","article-title":"\u03b2-Thalassemia distribution in the old world: an ancient disease seen from a historical standpoint","volume":"9","author":"De Sanctis","year":"2017","journal-title":"Mediterr J Hematol Infect Dis."},{"issue":"7801","key":"2020110202111180900_CIT0003","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S0140-6736(73)91878-3","article-title":"Differentiation of iron deficiency from thalassaemia trait by routine blood-count","volume":"1","author":"England","year":"1973","journal-title":"Lancet."},{"issue":"7808","key":"2020110202111180900_CIT0004","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/S0140-6736(73)91446-3","article-title":"Differentiation of iron deficiency from thalassaemia trait","volume":"1","author":"Mentzer","year":"1973","journal-title":"Lancet"},{"issue":"7821","key":"2020110202111180900_CIT0005","first-page":"154","article-title":"Differentiation of thalassaemia minor from iron deficiency","volume":"2","author":"Srivastava","year":"1973","journal-title":"Lancet."},{"issue":"12","key":"2020110202111180900_CIT0006","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1515\/cclm-2015-0179","article-title":"Discriminant indices for distinguishing thalassemia and iron deficiency in patients with microcytic anemia: a meta-analysis","volume":"53","author":"Hoffmann","year":"2015","journal-title":"Clin Chem Lab Med."},{"issue":"10","key":"2020110202111180900_CIT0007","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1515\/cclm-2016-0856","article-title":"Critical appraisal of discriminant formulas for distinguishing thalassemia from iron deficiency in patients with microcytic anemia","volume":"55","author":"Urrechaga","year":"2017","journal-title":"Clin Chem Lab Med."},{"issue":"2","key":"2020110202111180900_CIT0008","first-page":"1","article-title":"Discriminant functions in distinguishing beta thalassemia trait and iron deficiency anemia: the value of the RDW-SD","volume":"7","author":"Trivedi","year":"2010","journal-title":"Internet J Hematol."},{"issue":"5","key":"2020110202111180900_CIT0009","doi-asserted-by":"crossref","first-page":"355","DOI":"10.3109\/03630269.2014.948187","article-title":"A local equation for differential diagnosis of \u03b2-thalassemia trait and iron deficiency anemia by logistic regression analysis in Southeast Iran","volume":"38","author":"Sargolzaie","year":"2014","journal-title":"Hemoglobin."},{"key":"2020110202111180900_CIT0010","first-page":"67","article-title":"Hi & Ha, are new indices in differentiation between iron deficiency anemia and beta-thalassaemia trait. 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