{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:18:12Z","timestamp":1790360292339,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100013213","name":"ARUP Institute for Clinical and Experimental Pathology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013213","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Viscous body fluids present challenges during clinical laboratory testing. The present study was conducted to evaluate the effectiveness of hyaluronidase (HYAL) and ultracentrifugation (UC) pretreatment for a variety of body fluids before clinical chemistry testing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The following body fluids were evaluated: biliary\/hepatic, cerebrospinal, dialysate, drain, pancreatic, pericardial, peritoneal\/ascites, pleural, synovial, and vitreous. Analytes assessed included amylase, total bilirubin, cancer antigen 19-9, carcinoembryonic antigen, cholesterol, chloride, creatinine, glucose, lactate dehydrogenase, lipase, potassium, rheumatoid factor, sodium, total protein, triglycerides, urea nitrogen, and uric acid.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Observed percentage differences between HYAL treated and untreated fluids were less than \u00b115% for all analytes investigated, with a small number showing statistical significance (P &amp;lt;.05). In addition, UC showed increased variability for limited body fluid\/analyte combinations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The HYAL treatment effectively reduced viscosity for body fluids. Validation of specimen pretreatment processes ensures acceptable analytical performance and the absence of unanticipated interferences.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmaa115","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T02:12:25Z","timestamp":1607393545000},"page":"469-476","source":"Crossref","is-referenced-by-count":5,"title":["Pretreatment of Body Fluid Specimens Using Hyaluronidase and Ultracentrifugation"],"prefix":"10.1093","volume":"52","author":[{"given":"Sonia L","family":"La\u2019ulu","sequence":"first","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology , Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devon R","family":"Turner","sequence":"additional","affiliation":[{"name":"ARUP Laboratories , Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Zupan","sequence":"additional","affiliation":[{"name":"ARUP Laboratories , Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R","family":"Genzen","sequence":"additional","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology , Salt Lake City, Utah"},{"name":"ARUP Laboratories , Salt Lake City, Utah"},{"name":"Department of Pathology, University of Utah Health Sciences Center , Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"issue":"21","key":"2023020517411260700_CIT0001","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1016\/j.lfs.2007.02.037","article-title":"The magic glue hyaluronan and its eraser hyaluronidase: a biological overview","volume":"80","author":"Girish","year":"2007","journal-title":"Life Sci."},{"issue":"6","key":"2023020517411260700_CIT0002","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.biotechadv.2007.07.001","article-title":"The many ways to cleave hyaluronan","volume":"25","author":"Stern","year":"2007","journal-title":"Biotechnol Adv."},{"key":"2023020517411260700_CIT0003","first-page":"422","volume-title":"Appendix C: Body fluid diluent and pretreatment solutions. 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