{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:22:28Z","timestamp":1787728948254,"version":"build-2784847793"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rat bite fever and Haverhill fever are often difficult to diagnose in a clinical setting. This difficulty results in part from clinicians and laboratory professionals not being able to reliably recover the causative agent Streptobacillus moniliformis using culture-based methods. After utilizing an automated continuous-monitoring blood culture bottle system, we showed that the organism can be reliably cultured when a blood volume inoculum of 10 mL is used. Further, we showed that when the above recommendation is followed, sodium polyanethole sulfonate (up to a concentration of 0.05% w\/v) in commercially purchased blood culture bottle formulations seems to be inactivated, allowing for the growth and detection of S. moniliformis. Herein, we offer data and methods used to overcome these clinical limitations. This is a comprehensive study of the historical collection of S. moniliformis isolates maintained by our facility and believed to be the largest of its kind to date.<\/jats:p>","DOI":"10.1093\/labmed\/lmab009","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T12:13:58Z","timestamp":1610712838000},"page":"536-549","source":"Crossref","is-referenced-by-count":9,"title":["Using the BDFX40 Automated Continuous Blood Culture System to Isolate and Recover<i>Streptobacillus moniliformis<\/i>in the Presence of 0.05% SPS: A 55-Year, 56-Strain Retrospective Study"],"prefix":"10.1093","volume":"52","author":[{"given":"Adam M","family":"Szewc","sequence":"first","affiliation":[{"name":"Bacterial Special Pathogens Branch (BSPB), Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa E","family":"Bell","sequence":"additional","affiliation":[{"name":"Bacterial Special Pathogens Branch (BSPB), Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aubree J","family":"Kelly","sequence":"additional","affiliation":[{"name":"Bacterial Special Pathogens Branch (BSPB), Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben W","family":"Humrighouse","sequence":"additional","affiliation":[{"name":"Bacterial Special Pathogens Branch (BSPB), Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R","family":"McQuiston","sequence":"additional","affiliation":[{"name":"Bacterial Special Pathogens Branch (BSPB), Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"2022011223194663500_CIT0001","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1128\/CMR.00016-06","article-title":"Rat bite fever and Streptobacillus moniliformis","volume":"20","author":"Elliot","year":"2007","journal-title":"Clin Microbiol Rev."},{"key":"2022011223194663500_CIT0002","first-page":"680","article-title":"Rat bite fever.","volume-title":"Red Book: Report of the Committee on Infectious Diseases","author":"American Academy of Pediatrics","year":"2018","edition":"31"},{"key":"2022011223194663500_CIT0003","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1128\/9781555817381.ch35","article-title":"Aggregatibacter, Capnocytophaga, Eikenella, Kingella, Pasteurella, and other fastidious or rarely encountered Gram-negative rods.","volume-title":"Manual of Clinical Microbiology","author":"Zbinden","year":"2015","edition":"2"},{"key":"2022011223194663500_CIT0004","first-page":"539","volume-title":"Identification of Unusual Pathogenic Gram-negative Aerobic and Facultatively Anaerobic Bacteria","author":"Weyant","year":"1995","edition":"2"},{"key":"2022011223194663500_CIT0005","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1093\/ajcp\/60.6.854","article-title":"Streptobacillus moniliformis isolated from a case of Haverhill fever","volume":"60","author":"Lambe","year":"1973","journal-title":"Am J Clin 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In: Trujillo ME, Dedysh S, DeVos P, et al, eds. Bergey\u2019s Manual of Systematics of Archaea and Bacteria.","author":"Eisenberg"},{"key":"2022011223194663500_CIT0014","article-title":"Specimen collection, transport, and acceptability.","volume-title":"Clinical Microbiology Procedures Handbook","author":"Linscott","year":"2016","edition":"4"},{"issue":"11","key":"2022011223194663500_CIT0015","doi-asserted-by":"crossref","first-page":"3482","DOI":"10.1128\/JCM.02107-08","article-title":"Effects of volume and site of blood draw on blood culture results","volume":"47","author":"Gonsalves","year":"2009","journal-title":"J Clin Microbiol."},{"issue":"6","key":"2022011223194663500_CIT0016","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1128\/JCM.02919-15","article-title":"Diagnosis of bloodstream infections in children","volume":"54","author":"Dien Bard","year":"2016","journal-title":"J Clin Microbiol."}],"container-title":["Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/52\/6\/536\/42148737\/lmab009.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/52\/6\/536\/42148737\/lmab009.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T20:11:56Z","timestamp":1670789516000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/labmed\/article\/52\/6\/536\/6161539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,8]]},"references-count":16,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,3,8]]},"published-print":{"date-parts":[[2021,11,2]]}},"URL":"https:\/\/doi.org\/10.1093\/labmed\/lmab009","relation":{},"ISSN":["0007-5027","1943-7730"],"issn-type":[{"value":"0007-5027","type":"print"},{"value":"1943-7730","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,11,1]]},"published":{"date-parts":[[2021,3,8]]}}}