{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T12:28:08Z","timestamp":1776947288677,"version":"3.51.4"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:00:00Z","timestamp":1644624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Marie Sklodowska Curie Innovative Training Network Treatment","award":["H2020-MSCA-ITN-721236"],"award-info":[{"award-number":["H2020-MSCA-ITN-721236"]}]},{"name":"Uppsala University Hospital ALF"},{"name":"Stiftelsen Familjen Ernfors Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Research has shown that RNA extraction from adipose tissue (AT) is challenging because of high lipid content and low RNA quantity. We compared a traditional RNA extraction with a column-based method in human AT to evaluate RNA quantity and quality.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and Methods<\/jats:title>\n                  <jats:p>Human subcutaneous AT (n = 9) was collected through needle biopsy, and RNA was extracted using the phenol-chloroform traditional method and the RNeasy Lipid Tissue Mini Kit column-based method. The RNA quantity, quality, integrity, and expression of key AT genes were assessed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We found that the RNA quantity and integrity were reduced by 40% and 15-20%, respectively, using the column-based method compared to the traditional method, but the findings were not statistically significant. The column-based method showed a higher 260\/280 ratio (~2.0) compared to the traditional method (~1.8) (P &amp;lt;.05), suggesting lower amounts of contaminants. The expression of AT genes was comparable between methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The traditional extraction method provides adequate RNA yield and integrity compared to the column-based method, which is an advantage when AT specimens are small.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmab126","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T12:10:47Z","timestamp":1640088647000},"page":"e129-e133","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of RNA Isolation Methods in Human Adipose Tissue"],"prefix":"10.1093","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0341-7250","authenticated-orcid":false,"given":"Bipasha","family":"Nandi Jui","sequence":"first","affiliation":[{"name":"Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University , Uppsala , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assel","family":"Sarsenbayeva","sequence":"additional","affiliation":[{"name":"Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University , Uppsala , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Jernow","sequence":"additional","affiliation":[{"name":"Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University , Uppsala , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Hetty","sequence":"additional","affiliation":[{"name":"Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University , Uppsala , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5498-3899","authenticated-orcid":false,"given":"Maria J","family":"Pereira","sequence":"additional","affiliation":[{"name":"Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University , Uppsala , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,2,12]]},"reference":[{"issue":"3","key":"2022090114002540500_CIT0001","doi-asserted-by":"crossref","first-page":"R77","DOI":"10.1530\/JOE-16-0211","article-title":"Adipose tissue in control of metabolism","volume":"231","author":"Luo","year":"2016","journal-title":"J Endocrinol."},{"key":"2022090114002540500_CIT0002","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ab.2017.12.020","article-title":"Modifications in routine protocol of RNA isolation can improve quality of RNA purified from adipocytes","volume":"543","author":"Sinitsky","year":"2018","journal-title":"Anal Biochem."},{"key":"2022090114002540500_CIT0003","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1186\/1756-0500-6-472","article-title":"Highly efficient method for isolation of total RNA from adipose 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the false discovery rate","volume":"93(3)","author":"Benjamini","year":"2006","journal-title":"Biometrika."},{"key":"2022090114002540500_CIT0014","article-title":"RNeasy lipid tissue mini handbook","author":"Qiagen","year":"2018"},{"issue":"5","key":"2022090114002540500_CIT0015","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1002\/nur.20448","article-title":"Collection and storage of human blood and adipose for genomic analysis of clinical samples","volume":"34","author":"Cashion","year":"2011","journal-title":"Res Nurs Health."},{"issue":"3","key":"2022090114002540500_CIT0016","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s10561-016-9600-7","article-title":"Effect of multiple cycles of freeze-thawing on the RNA quality of lung cancer tissues","volume":"18","author":"Yu","year":"2017","journal-title":"Cell Tissue 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