{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T12:19:15Z","timestamp":1778933955892,"version":"3.51.4"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T00:00:00Z","timestamp":1670630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>This study was conducted to estimate prevalence of direct antiglobulin test (DAT) positivity and its impact on transfusion support in patients with thalassemia.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The DAT testing was performed for patients with \u03b2-thalassemia who received transfusion from November 2021 to March 2022. Elution was done for DAT-positive samples.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 180 patients, 21 (11.6%) were DAT positive. Immunoglobulin G (IgG) was present in 4 (19%) and IgG+C3d was present in 8 (38%). Only complement was present in 9 (42.8%) patients. The IgG-reactive DATs were associated with pan-reactive eluate. Patients who were DAT-positive had significantly higher levels of serum bilirubin, ferritin, and IgG than those who were DAT-negative.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Autoantibody formation in multiply transfused thalassemia patients is common and merits equal attention as alloimmunization. It is particularly important as DAT-positive red blood\u00a0cells may undergo clinically significant hemolysis, which may increase the transfusion requirements with associated sequalae such as increased serum ferritin and splenomegaly.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmac140","type":"journal-article","created":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T11:13:07Z","timestamp":1670670787000},"page":"406-410","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence of the\u00a0Direct Antiglobulin Test and Its Clinical Impact on Multiply Transfused Thalassemia Patients: A\u00a0Prospective Study Conducted at a\u00a0Tertiary Care Center in Northern India"],"prefix":"10.1093","volume":"54","author":[{"given":"Brijesh","family":"Kumar\u00a0Yadav","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendra\u00a0K","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harsha","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priti","family":"Elhence","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute Medical Sciences, Lucknow , 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