{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T05:05:32Z","timestamp":1732251932080,"version":"3.28.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Single-platform flow cytometry technology together with CD45-gating is becoming the method of choice for absolute CD4 T cell enumeration. Immunological assessment of HIV patients by monitoring CD4 can provide valuable information on antiviral treatment response and disease progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 97 HIV-positive individuals were recruited from 2 hospitals in Tripoli, Libya, and 14 healthy blood donors. The HIV-infected individuals were classified by CD4+ count into HIV-positive (&amp;gt;200 cells\/\u00b5L) or AIDS (\u2264200 cells\/\u00b5L) groups. CD4+ and CD8+ cell counts were determined and compared among the groups and with similar published data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean \u00b1 SD CD4+ cell counts were 1106 \u00b1 442.8 cells\/\u00b5L in healthy individuals, 460 \u00b1 219.7 cells\/\u00b5L in the HIV-positive group, and 78 \u00b1 64.3 cells\/\u00b5L in the AIDS group. The mean \u00b1 SD CD4+\/CD8+ ratio was 1.6 \u00b1 0.58, 0.4 \u00b1 0.22, and 0.1 \u00b1 0.1, respectively. CD4+ counts in Libyan healthy adults might be higher than those reported in several studies in other regions, whereas CD4+ counts in Libyan AIDS patients seem lower.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reference values for T lymphocyte counts in Libyan healthy individuals should be investigated more extensively, and the reasons why Libyan AIDS patients seem to have such lower CD4+ counts should be examined.<\/jats:p><\/jats:sec>","DOI":"10.1093\/labmed\/lmae046","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T05:39:37Z","timestamp":1718948377000},"page":"763-767","source":"Crossref","is-referenced-by-count":0,"title":["Absolute CD4 count and percentage values among Libyan patients with HIV by single-platform flow cytometry"],"prefix":"10.1093","volume":"55","author":[{"given":"Yosra","family":"Lamami","sequence":"first","affiliation":[{"name":"Libyan Biotechnology Research Center, Department of Human Cells and Tissues , Tripoli ,","place":["Libya"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulmunem M","family":"Abulayha","sequence":"additional","affiliation":[{"name":"Libyan Biotechnology Research Center, Department of Human Cells and Tissues , Tripoli ,","place":["Libya"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah","family":"Altabal","sequence":"additional","affiliation":[{"name":"Libyan Biotechnology Research Center, Department of Human Cells and Tissues , Tripoli ,","place":["Libya"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Elbasir","sequence":"additional","affiliation":[{"name":"Libyan Biotechnology Research Center, Department of Human Cells and Tissues , Tripoli ,","place":["Libya"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulrhman S","family":"Elbnnani","sequence":"additional","affiliation":[{"name":"Libyan Biotechnology Research Center, Department of Human Cells 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