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In fact, there is a broad range of variables that can directly or indirectly affect test results, either individually or collectively. It is therefore crucial to identify the ideal requirements to achieve reliable results as well as using these requirements to provide standard operating procedures that should be taken into account. Therefore, we focus on aligning optimum results by comparing preanalytical and analytical phases that influence test results, such as the effect of various anticoagulants, transport and maintaining temperature (24\u00b0C or 4\u00b0C) of samples, test prime run time, appropriate solution concentrations, and effect of incubation temperature (24\u00b0C or 37\u00b0C) during the test run.<\/jats:p>","DOI":"10.1093\/labmed\/lmae076","type":"journal-article","created":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T14:45:46Z","timestamp":1725806746000},"page":"150-163","source":"Crossref","is-referenced-by-count":3,"title":["Dihydrorhodamine-123 flow cytometry method: time for substantial revision in technical procedure"],"prefix":"10.1093","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6668-0366","authenticated-orcid":false,"given":"Mahdi","family":"Zavvar","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Allied Medical 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