{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:43:58Z","timestamp":1783442638578,"version":"3.54.6"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8177080584"],"award-info":[{"award-number":["8177080584"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Meningiomas are the most common intracranial tumors and their diagnosis relies mostly on neuroimaging and histology. However, the histology grades cannot predict the outcome exactly and some meningiomas tend to recur after resection of even benign tumors. Therefore, it is necessary to explore prognostic and diagnostic molecular targets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Differential expression analysis between meningiomas and meninges was performed based on the merged data of GSE43290 and GSE84263. Next, we performed gene set enrichment analysis (GSEA), immune cell infiltration analysis, protein-protein interaction analysis, and survival analysis using public data. The expression level of Synaptosome-associated-protein-25kDa (SNAP25) was verified by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and Western blotting in meningioma tissues.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>There were 263 upregulated and 592 downregulated genes identified in meningiomas by differential expression analysis. GSEA results revealed that meningiomas were negatively related to the pathway of soluble N-ethylmaleimide sensitive factor attachment protein receptor interactions in vascular transport and chemokine signaling. SNAP25 was characterized as a hub gene and downregulated in meningiomas. The Kaplan-Meier plot indicated that high expression of SNAP25 is a favorable factor.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>SNAP25 was downregulated and identified as a potential prognostic marker in meningioma.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmae085","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T18:32:42Z","timestamp":1731090762000},"page":"238-248","source":"Crossref","is-referenced-by-count":1,"title":["SNAP25 as a prognostic marker in transcriptome analysis of meningioma"],"prefix":"10.1093","volume":"56","author":[{"given":"Yu","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine , Shanghai, 200123 ,","place":["China"]},{"name":"Department of Laboratory Medicine, Huashan Hospital, Fudan University , Shanghai 200040 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Department of Laboratory Medicine, , Shanghai, 200011 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"issue":"8","key":"2025051000265493500_CIT0001","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1093\/neuonc\/noab106","article-title":"The 2021 WHO classification of tumors of the central nervous system: a summary","volume":"23","author":"Louis","year":"2021","journal-title":"Neuro Oncol."},{"issue":"12 Suppl 2","key":"2025051000265493500_CIT0002","doi-asserted-by":"publisher","first-page":"iv1","DOI":"10.1093\/neuonc\/noad149","article-title":"CBTRUS statistical report: primary brain and other central nervous system tumors diagnosed in the United States in 2016-2020","volume":"25","author":"Ostrom","year":"2023","journal-title":"Neuro Oncol."},{"issue":"5","key":"2025051000265493500_CIT0003","doi-asserted-by":"publisher","first-page":"101562","DOI":"10.1016\/j.canep.2019.07.001","article-title":"Incidence, mortality and outcome of meningiomas: a population-based study from Germany","volume":"62","author":"Holleczek","year":"2019","journal-title":"Cancer Epidemiol"},{"issue":"11","key":"2025051000265493500_CIT0004","doi-asserted-by":"publisher","first-page":"1821","DOI":"10.1093\/neuonc\/noab150","article-title":"EANO guideline on the diagnosis and management of meningiomas","volume":"23","author":"Goldbrunner","year":"2021","journal-title":"Neuro Oncol"},{"issue":"1","key":"2025051000265493500_CIT0005","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1002\/cam4.1531","article-title":"Efficacy of adjuvant radiotherapy for atypical and anaplastic meningioma","volume":"8","author":"Zhu","year":"2019","journal-title":"Cancer Med"},{"issue":"4","key":"2025051000265493500_CIT0006","doi-asserted-by":"publisher","first-page":"1978","DOI":"10.3390\/ijms23041978","article-title":"mTOR signaling and potential therapeutic targeting in meningioma","volume":"23","author":"Pinker","year":"2022","journal-title":"Int J Mol Sci ."},{"issue":"5","key":"2025051000265493500_CIT0007","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1093\/neuonc\/noaa226","article-title":"Associations of meningioma molecular subgroup and tumor recurrence","volume":"23","author":"Youngblood","year":"2021","journal-title":"Neuro Oncol"},{"issue":"16","key":"2025051000265493500_CIT0008","doi-asserted-by":"publisher","first-page":"4218","DOI":"10.1158\/0008-5472.can-20-3669","article-title":"Loss-of-function mutations in TRAF7 and KLF4 cooperatively activate RAS-Like GTPase signaling and promote meningioma development","volume":"81","author":"Najm","year":"2021","journal-title":"Cancer Res."},{"issue":"10","key":"2025051000265493500_CIT0009","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1038\/ng.3651","article-title":"Recurrent somatic mutations in POLR2A define a distinct subset of meningiomas","volume":"48","author":"Clark","year":"2016","journal-title":"Nat Genet."},{"issue":"2","key":"2025051000265493500_CIT0010","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1038\/nrneurol.2017.168","article-title":"Advances in meningioma genetics: novel therapeutic opportunities","volume":"14","author":"Preusser","year":"2018","journal-title":"Nat Rev Neurol."},{"issue":"4","key":"2025051000265493500_CIT0011","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1093\/jmcb\/mjx023","article-title":"KLF4 is a tumor suppressor in anaplastic meningioma stem-like cells and human meningiomas","volume":"9","author":"Tang","year":"2017","journal-title":"J Mol Cell Biol."},{"issue":"2","key":"2025051000265493500_CIT0012","doi-asserted-by":"publisher","first-page":"e245","DOI":"10.1016\/j.wneu.2021.10.163","article-title":"NF2 gene participates in regulation of the cell cycle of meningiomas by restoring spindle assembly checkpoint function and inhibiting the binding of Cdc20 protein to anaphase promoting complex\/cyclosome","volume":"158","author":"Ma","year":"2022","journal-title":"World Neurosurg"},{"key":"2025051000265493500_CIT0013","doi-asserted-by":"publisher","first-page":"698835","DOI":"10.3389\/fonc.2021.698835","article-title":"SNAP25 inhibits glioma progression by regulating synapse plasticity via GLS-mediated glutaminolysis","volume":"11","author":"Huang","year":"2021","journal-title":"Front Oncol."},{"key":"2025051000265493500_CIT0014","doi-asserted-by":"publisher","first-page":"e10142","DOI":"10.7717\/peerj.10142","article-title":"Mining the potential prognostic value of synaptosomal-associated protein 25 (SNAP25) in colon cancer based on stromal-immune score","volume":"8","author":"Zou","year":"2020","journal-title":"PeerJ."},{"issue":"6","key":"2025051000265493500_CIT0015","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1093\/bioinformatics\/bts034","article-title":"The sva package for removing batch effects and other unwanted variation in high-throughput experiments","volume":"28","author":"Leek","year":"2012","journal-title":"Bioinformatics."},{"issue":"7","key":"2025051000265493500_CIT0016","doi-asserted-by":"publisher","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"issue":"43","key":"2025051000265493500_CIT0017","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U S A."},{"issue":"3","key":"2025051000265493500_CIT0018","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/ng1180","article-title":"PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes","volume":"34","author":"Mootha","year":"2003","journal-title":"Nat Genet."},{"issue":"1","key":"2025051000265493500_CIT0019","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/1755-8794-2-18","article-title":"PrognoScan: a new database for meta-analysis of the prognostic value of genes","volume":"2","author":"Mizuno","year":"2009","journal-title":"BMC Med Genomics."},{"issue":"5","key":"2025051000265493500_CIT0020","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.semcancer.2008.03.004","article-title":"Macrophage polarization in tumour progression","volume":"18","author":"Sica","year":"2008","journal-title":"Semin Cancer Biol."},{"issue":"1","key":"2025051000265493500_CIT0021","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.jhep.2022.02.019","article-title":"PKC\u03b1\/ZFP64\/CSF1 axis resets the tumor microenvironment and fuels anti-PD1 resistance in hepatocellular carcinoma","volume":"77","author":"Wei","year":"2022","journal-title":"J Hepatol."},{"issue":"5","key":"2025051000265493500_CIT0022","doi-asserted-by":"publisher","first-page":"eabm6247","DOI":"10.1126\/sciadv.abm6247","article-title":"Multiple approaches converge on three biological subtypes of meningioma and extract new insights from published studies","volume":"8","author":"Bayley","year":"2022","journal-title":"Sci Adv."},{"issue":"6","key":"2025051000265493500_CIT0023","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1007\/s00401-016-1545-1","article-title":"The 2016 World Health Organization classification of tumors of the central nervous system: a summary","volume":"131","author":"Louis","year":"2016","journal-title":"Acta Neuropathol."},{"issue":"18","key":"2025051000265493500_CIT0024","doi-asserted-by":"publisher","first-page":"4707","DOI":"10.3390\/cancers13184707","article-title":"The value of SSTR2 receptor-targeted PET\/CT in proton irradiation of grade I meningioma","volume":"13","author":"Pelak","year":"2021","journal-title":"Cancers (Basel)"},{"issue":"1","key":"2025051000265493500_CIT0025","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/s13014-021-01825-2","article-title":"Retrospective analysis of recurrence patterns and clinical outcome of grade II meningiomas following postoperative radiotherapy","volume":"16","author":"Hoffmann","year":"2021","journal-title":"Radiat Oncol."},{"issue":"1","key":"2025051000265493500_CIT0026","doi-asserted-by":"publisher","first-page":"16432","DOI":"10.1038\/s41598-021-95956-x","article-title":"WHO grade I meningiomas that show regrowth after gamma knife radiosurgery often show 1p36 loss","volume":"11","author":"Damen","year":"2021","journal-title":"Sci Rep."},{"issue":"10","key":"2025051000265493500_CIT0027","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.1158\/2159-8290.cd-23-1459","article-title":"Perivascular NOTCH3+ stem cells drive meningioma tumorigenesis and resistance to radiotherapy","volume":"14","author":"Choudhury","year":"2024","journal-title":"Cancer Discov"},{"issue":"1","key":"2025051000265493500_CIT0028","doi-asserted-by":"publisher","first-page":"100157","DOI":"10.1074\/jbc.ra120.014960","article-title":"mTOR kinase inhibition disrupts neuregulin 1-ERBB3 autocrine signaling and sensitizes NF2-deficient meningioma cellular models to IGF1R inhibition","volume":"296","author":"Beauchamp","year":"2021","journal-title":"J Biol Chem."},{"issue":"11","key":"2025051000265493500_CIT0029","doi-asserted-by":"publisher","first-page":"1922","DOI":"10.1093\/neuonc\/noab075","article-title":"Targeting the CSF1\/CSF1R axis is a potential treatment strategy for malignant meningiomas","volume":"23","author":"Yeung","year":"2021","journal-title":"Neuro-Oncol."},{"issue":"1","key":"2025051000265493500_CIT0030","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1093\/neuonc\/noab118","article-title":"Activity of PD-1 blockade with nivolumab among patients with recurrent atypical\/anaplastic meningioma: phase II trial results","volume":"24","author":"Bi","year":"2022","journal-title":"Neuro Oncol"},{"issue":"5","key":"2025051000265493500_CIT0031","doi-asserted-by":"publisher","first-page":"755-767","DOI":"10.1093\/neuonc\/noab243","article-title":"Trabectedin for recurrent WHO grade 2 or 3 meningioma: a randomized phase 2 study of the EORTC Brain Tumor Group (EORTC-1320-BTG)","volume":"24","author":"Preusser","year":"2022","journal-title":"Neuro Oncol"},{"issue":"2","key":"2025051000265493500_CIT0032","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s00401-020-02247-2","article-title":"Clear cell meningiomas are defined by a highly distinct DNA methylation profile and mutations in SMARCE1","volume":"141","author":"Sievers","year":"2020","journal-title":"Acta Neuropathol."},{"issue":"5","key":"2025051000265493500_CIT0033","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1016\/s1470-2045(17)30155-9","article-title":"DNA methylation-based classification and grading system for meningioma: a multicentre, retrospective analysis","volume":"18","author":"Sahm","year":"2017","journal-title":"Lancet Oncol."},{"issue":"7874","key":"2025051000265493500_CIT0034","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1038\/s41586-021-03850-3","article-title":"A clinically applicable integrative molecular classification of meningiomas","volume":"597","author":"Nassiri","year":"2021","journal-title":"Nature."},{"issue":"5","key":"2025051000265493500_CIT0035","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1093\/neuonc\/noab213","article-title":"A molecularly integrated grade for meningioma","volume":"24","author":"Driver","year":"2022","journal-title":"Neuro Oncol"},{"issue":"2","key":"2025051000265493500_CIT0036","doi-asserted-by":"publisher","first-page":"1880","DOI":"10.1002\/jcb.29423","article-title":"SNHG1\/miR-556-5p\/TCF12 feedback loop enhances the tumorigenesis of meningioma through Wnt signaling pathway","volume":"121","author":"Zhang","year":"2020","journal-title":"J Cell Biochem."},{"issue":"1","key":"2025051000265493500_CIT0037","doi-asserted-by":"publisher","first-page":"537763","DOI":"10.3389\/fonc.2020.537763","article-title":"Long non-coding rNA MEG3 modifies cell-cycle, migration, invasion, and proliferation through AKAP12 by sponging miR-29c in meningioma cells","volume":"10","author":"Ding","year":"2020","journal-title":"Front Oncol."},{"key":"2025051000265493500_CIT0038","doi-asserted-by":"publisher","first-page":"108718","DOI":"10.1016\/j.biopha.2019.108718","article-title":"LINC00702\/miR-4652-3p\/ZEB1 axis promotes the progression of malignant meningioma through activating Wnt\/\u03b2-catenin pathway","volume":"113","author":"Li","year":"2019","journal-title":"Biomed Pharmacother."},{"key":"2025051000265493500_CIT0039","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.biopha.2018.06.005","article-title":"Long noncoding RNA LINC00460 targets miR-539\/MMP-9 to promote meningioma progression and metastasis","volume":"105","author":"Xing","year":"2018","journal-title":"Biomed Pharmacother."},{"issue":"6 Pt 1","key":"2025051000265493500_CIT0040","doi-asserted-by":"publisher","first-page":"3039","DOI":"10.1083\/jcb.109.6.3039","article-title":"The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations","volume":"109","author":"Oyler","year":"1989","journal-title":"J Cell Biol."},{"issue":"6","key":"2025051000265493500_CIT0041","doi-asserted-by":"publisher","first-page":"BSR20200516","DOI":"10.1042\/bsr20200516","article-title":"Association between SNAP25 and human glioblastoma multiform: a comprehensive bioinformatic analysis","volume":"40","author":"Yu","year":"2020","journal-title":"Biosci Rep."},{"issue":"5","key":"2025051000265493500_CIT0042","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.3892\/ijmm.2017.2925","article-title":"Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma","volume":"39","author":"Huang","year":"2017","journal-title":"Int J Mol Med."},{"issue":"1","key":"2025051000265493500_CIT0043","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/ejp.904","article-title":"Cleavage of SNAP-25 ameliorates cancer pain in a mouse model of melanoma","volume":"21","author":"Olbrich","year":"2017","journal-title":"Eur J Pain."},{"issue":"16","key":"2025051000265493500_CIT0044","doi-asserted-by":"publisher","first-page":"3499","DOI":"10.1158\/1078-0432.ccr-21-3972","article-title":"Serum concentration of CD137 and tumor infiltration by M1 macrophages predict the response to sintilimab plus bevacizumab biosimilar in advanced hepatocellular carcinoma patients","volume":"28","author":"Zhang","year":"2022","journal-title":"Clin Cancer Res."},{"issue":"4","key":"2025051000265493500_CIT0045","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1002\/cac2.12276","article-title":"Combination of anti-PD-L1 antibody with peptide MEL-dKLA targeting M2 tumor-associated macrophages suppresses breast cancer metastasis","volume":"42","author":"Lee","year":"2022","journal-title":"Cancer Commun (Lond)"},{"issue":"5","key":"2025051000265493500_CIT0046","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1016\/j.immuni.2021.01.012","article-title":"Chemokines and the immune response to cancer","volume":"54","author":"Ozga","year":"2021","journal-title":"Immunity."}],"container-title":["Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/56\/3\/238\/60543149\/lmae085.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/56\/3\/238\/60543149\/lmae085.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T04:26:57Z","timestamp":1746851217000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/labmed\/article\/56\/3\/238\/7887688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,8]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,11,8]]},"published-print":{"date-parts":[[2025,5,9]]}},"URL":"https:\/\/doi.org\/10.1093\/labmed\/lmae085","relation":{},"ISSN":["0007-5027","1943-7730"],"issn-type":[{"value":"0007-5027","type":"print"},{"value":"1943-7730","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,5]]},"published":{"date-parts":[[2024,11,8]]}}}