{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:07:45Z","timestamp":1792638465119,"version":"4.5.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The multipositivity of myositis-specific antibodies (MSAs) is uncommon. Our study aimed to assess the prevalence and the clinical relevance of multiple positive MSAs in routine practice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A 10 year single-center retrospective study (2015-2024) was conducted reviewing all samples analyzed with the Dot Myositis EUROLINE (Euroimmun Germany). Clinical data corresponding to samples with multiple positive MSAs was reviewed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 140 samples positive for at least 1 MSA, 22 patients (15.7%) were positive for at least 2 MSAs on the same sample. The diagnosis of idiopathic inflammatory myopathies (IIMs) was confirmed in only 6 patients (27.2%). The frequency of double-positive MSAs was 100% in IIM patients and 60% in non-IIM patients. No IIM patients and 40% of non-IIM patients (P = .037) had 3 or more positive MSAs. MSA titers were higher in IIM patients than in non-IIM patients (49 vs 32 AU, P = .33). The clinical IIM subtype joined the IIM subtype corresponding to the predominant MSA. We had no mixed features of either IIM subtype or added severe prognosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Detection of multiple MSAs is uncommon but possible using immunodot techniques. Taking into account MSA number, level of positivity, and clinical data helps in the interpretation of the results.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmae103","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T13:16:47Z","timestamp":1736169407000},"page":"367-374","source":"Crossref","is-referenced-by-count":3,"title":["Detection of multiple myositis-specific antibodies: prevalence and clinical significance"],"prefix":"10.1093","volume":"56","author":[{"given":"Ameni","family":"Jerbi","sequence":"first","affiliation":[{"name":"Immunology Department, Habib Bourguiba University Hospital, University of Sfax , Sfax ,","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Karoui","sequence":"additional","affiliation":[{"name":"Immunology Department, Habib Bourguiba University Hospital, University of Sfax , Sfax 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