{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T13:26:06Z","timestamp":1763817966953,"version":"3.45.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>This retrospective cross-sectional study aimed to evaluate the differences between the restriction fragment length polymorphism (RFLP) patterns of the dormancy survival regulator (DosR) regulon in latent tuberculosis vs active disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sputum samples from 90 patients with active Mycobacterium tuberculosis infection were collected. The presence of the devR, devS, and dosT genes in active and induced dormant M tuberculosis infection was evaluated using polymerase chain reaction (PCR). In addition, the differences between the restriction enzyme digestion of these genes were determined using the RFLP method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The devR gene was much more prevalent in dormant than in active samples, with statistical significance set at P\u2009=\u2009.033. The PCR-RFLP patterns obtained from the effect of the AciI endonuclease on devR, the EaeI and HincII endonucleases on devS, and the HaeIII and AciI endonucleases on dosT showed a statistically significant difference between the active and dormant groups (P\u2005=\u2005.001, P\u2005=\u2005.01, P\u2005=\u2005.008, P\u2005=\u2005.001, and P\u2005=\u2005.001, respectively), and this difference was associated with more diverse patterns in the active group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Results suggested that the DosR regulon may have more nucleotide variations at the active stage. This study is the first to investigate the devR, devS, and dosT genes using PCR-RFLP as a cost-effective and straightforward method.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmaf046","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T07:20:21Z","timestamp":1750663221000},"page":"766-773","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of active and dormant\n                    <i>Mycobacterium tuberculosis<\/i>\n                    DosR\/DevR regulon polymerase chain reaction\u2013restriction fragment length polymorphism patterns"],"prefix":"10.1093","volume":"56","author":[{"given":"Faezeh","family":"Hamidieh","sequence":"first","affiliation":[{"name":"Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences , Tehran 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