{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:28:21Z","timestamp":1772641701541,"version":"3.50.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Sichuan Province Medical Science Research Project Program","award":["S23047"],"award-info":[{"award-number":["S23047"]}]},{"name":"Health Commission of Sichuan Province Medical Science Technology Program","award":["24WSXT093"],"award-info":[{"award-number":["24WSXT093"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>We sought to assess the clinical utility of methylation detection of paired box gene 1 (PAX1) and junctional adhesion molecule 3 (JAM3) in the triage of individuals testing positive for high-risk human papillomavirus (HPV).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cervical secretions from 312 high-risk HPV\u2013positive patients were analyzed for dual-gene methylation of PAX1 and JAM3 (PAX1m\/JAM3m). Methylation levels were compared across histologically confirmed cervical lesions. Using histopathology as the reference standard, the triage performance of PAX1m\/JAM3m was evaluated against cytology and HPV-16\/18 genotyping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Methylation positivity increased in statistical significance with lesion severity (P\u2009&amp;lt;\u2009.001 for trend). For the detection of cervical intraepithelial neoplasia (CIN) 2 or more severe lesions (CIN2+), PAX1m\/JAM3m yielded a sensitivity of 91.8% (95% CI, 84.1%-96.2%), specificity of 90.7% (95% CI, 85.7%-94.1%), and an area under the receiver operating characteristic curve of 0.912 (95% CI, 0.874-0.951), outperforming cytology, HPV-16\/18 genotyping, and their combinations. Using PAX1m\/JAM3m positivity as a criterion for colposcopy referral, 1 CIN2+ case was detected per 1.22 referrals, reducing the colposcopy referral rate by approximately 19.2% and increasing the CIN2+ detection rate by 39.4% compared with cytology at the atypical squamous cells of undetermined significance threshold.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>PAX1m\/JAM3m levels are strongly associated with cervical lesion severity and represent a promising triage strategy for high-risk HPV\u2013positive individuals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmaf096","type":"journal-article","created":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T12:43:23Z","timestamp":1767271403000},"source":"Crossref","is-referenced-by-count":0,"title":["Utility of\n                    <i>PAX1<\/i>\n                    \/\n                    <i>JAM3<\/i>\n                    methylation analysis for triage of high-risk HPV-positive individuals"],"prefix":"10.1093","volume":"57","author":[{"given":"Qin","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaguo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Diao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinghong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyun","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zejiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3596-8739","authenticated-orcid":false,"given":"Xuhui","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Ziyang Central Hospital , Ziyang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"2026030406391728500_lmaf096-B1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1093\/emph\/eov003","article-title":"Papillomaviruses: viral evolution, cancer and evolutionary medicine","volume":"2015","author":"Bravo","year":"2015","journal-title":"Evol Med Public 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