{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T11:59:46Z","timestamp":1773316786218,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Standard triglyceride assays may overestimate true triglyceride concentrations due to endogenous glycerol interference, particularly in type 2 diabetes, where enhanced lipolysis elevates circulating free glycerol. We compared glycerol-blanked and non-blanked triglyceride assays in patients with type 2 diabetes to quantify analytical bias and assess impact on cardiovascular risk indices.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This cross-sectional study enrolled 200 patients with type 2 diabetes and 200 healthy control individuals. Serum triglyceride levels were measured using glycerol-blanked (Sekisui) and nonblanked (Beckman Coulter) enzymatic assays. Bias assessment, correlation analysis, and impact on atherogenic index of plasma were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Median nonblanked triglyceride values were substantially higher than glycerol-blanked triglyceride values in control individuals (129.5 vs 120.95\u2009mg\/dL, P\u2009=\u2009.02) and patients with type 2 diabetes (158.5 vs 136.85\u2009mg\/dL, P\u2009=\u2009.0019). Overestimation was greater in patients (median difference, 16.1\u2009mg\/dL) than in control individuals (9.95\u2009mg\/dL, P\u2009=\u2009.0001). Bland-Altman analysis showed higher mean (SD) bias in patients with type 2 diabetes (\u221221.25 [41.51]\u2009mg\/dL) than in control individuals (\u221213.26 [31.26]\u2009mg\/dL). The triglyceride\u2013 glycerol-blanked triglyceride difference correlated with glycated hemoglobin values in individuals with type 2 diabetes (\u03c1\u2009=\u20090.141, P\u2009=\u2009.0473).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Glycerol-blanked triglyceride assays provide more accurate lipid assessment in type 2 diabetes, improving cardiovascular risk stratification and therapeutic decision-making.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag003","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:45:52Z","timestamp":1769604352000},"source":"Crossref","is-referenced-by-count":0,"title":["Assessment of glycerol-related analytical bias in triglyceride estimation for patients with type 2 diabetes"],"prefix":"10.1093","volume":"57","author":[{"given":"Mohini","family":"Rathore","sequence":"first","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7106-8545","authenticated-orcid":false,"given":"Manoj","family":"Khokhar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0661-9854","authenticated-orcid":false,"given":"Maya","family":"Gopalakrishnan","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shrimanjunath","family":"Sankanagoudar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mithu","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , Jodhpur,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, 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