{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:55:14Z","timestamp":1774486514929,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Jiangsu Health Innovation Team Program"},{"name":"Jiangsu Transfusion Association \u201cAikang MedTech\u201d Project","award":["JSAK2023012"],"award-info":[{"award-number":["JSAK2023012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>An 85-year-old O D-positive male with a history of multiple D-positive RBC transfusions (10.5 units in 2020, 5.5 units in 2023) presented with severe anemia in June 2024. Unexpected antibodies were found in his plasma, and crossmatching was incompatible with all O D-positive donor RBCs. In this article, we document the methodology for confirming a hidden partial D antigen with rare RHD*DV.1 allele variant, with the objectives of elucidating the characteristics of this rare allele and establishing evidence-based transfusion guidelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This partial D antigen was identified using column agglutination and tube methods for the patient in this case. Subsequent adsorption-elution assays characterized the antibody mixture in the his plasma. RHD gene sequencing confirmed the specific mutations and zygosity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with the 4+ agglutination observed in column agglutination testing for the patient in this instance, a much weaker reaction (2+ agglutination) was noted in tube testing. RHD gene sequencing revealed 2 variations in exon 5\u2014c.667T&amp;gt;G and c.697G&amp;gt;C\u2014consistent with the RHD*DV.1 allele. Both of the patient\u2019s sons were genotyped as RHD*DV.1\/RHD*01, confirming the inheritance of this allele from their father. Analysis of the patient\u2019s haplotypes showed only 1 single-nucleotide variation (formerly single-nucleotide polymorphism) difference (c.801\u2009+\u2009108G&amp;gt;A) located in intron 5. This finding demonstrates that the 2 exon 5 variations reside on separate haplotypes, confirming that this patient was a definitive homozygote for RHD*DV.1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>For women of childbearing potential, pretransfusion screening using the tube method remains a vital component in the early detection of D antigen variants and is essential for preventing alloimmunization.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag005","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:15:39Z","timestamp":1774484139000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification and pedigree analysis of a hidden partial D antigen with rare\n                    <i>RHD*DV.1<\/i>\n                    allele"],"prefix":"10.1093","volume":"57","author":[{"given":"Xiaohua","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Blood Transfusion, Affiliated Maternity and Child Health Care Hospital of Nantong University , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Affiliated Maternity and Child Health Care Hospital of Nantong University , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunzi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Affiliated Maternity and Child Health Care Hospital of Nantong University , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis Center, Affiliated Maternity and Child Health Care Hospital of Nantong University , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoye","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Affiliated Hospital of Nantong University , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kecheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory, Nantong Blood Center , Nantong,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"2026032520153175200_lmag005-B1","doi-asserted-by":"publisher","first-page":"857","DOI":"10.13303\/j.cjbt.issn.1004-549x.2023.10.001","article-title":"The challenge of finding Chinese blood groups","volume":"36","author":"Zhao","year":"2023","journal-title":"Chin J Blood 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