{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T23:53:39Z","timestamp":1785887619753,"version":"3.56.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>This study retrospectively characterized the laboratory features of direct antiglobulin test (DAT)\u2013positive anemia in newly diagnosed multiple myeloma (MM) and explored associated immunologic alterations to better understand MM-related immune dysregulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We enrolled 77 patients with newly diagnosed MM admitted to our hospital between 2021 and 2025. Based on DAT results, patients were assigned to a DAT-positive group (n\u2009=\u200952) or a DAT-negative group (n\u2009=\u200925). Clinical characteristics, laboratory parameters, and peripheral blood T-cell subsets were compared between groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Monoclonal immunoglobulin (M protein) isotype distribution was statistically significantly different between groups (P\u2009&amp;lt;\u2009.001), with immunoglobulin G predominating in the DAT-positive group (88.46%). Compared with the DAT-negative group, DAT-positive patients had lower albumin, sodium, and potassium levels as well as a lower albumin to globulin ratio but higher levels of globulin and ferritin as well as higher absolute lymphocyte counts (all P\u2009&amp;lt;\u2009.05). Flow cytometric analysis showed increased T-cell\u2013related parameters, particularly CD8-positive T cells, in the DAT-positive group, whereas reduced natural killer\u2013cell and B-cell counts were more common in the DAT-negative group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The observed laboratory profile suggested a more pronounced state of inflammatory or immune activation and electrolyte disturbances, highlighting the need for improved clinical recognition and detection of potential immune-mediated red blood cell injury, hemolytic predisposition, and related immune abnormalities.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag044","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T12:01:10Z","timestamp":1784721670000},"source":"Crossref","is-referenced-by-count":0,"title":["Laboratory characteristics of direct antiglobulin test\u2013positive anemia in patients with newly diagnosed multiple myeloma"],"prefix":"10.1093","volume":"57","author":[{"given":"Yutong","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YanTian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Huo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChuanYing","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YongQi","family":"He","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YinChu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HongShuai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JunQin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YaJun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chaoyang Hospital, Capital Medical University , 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