{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T19:13:22Z","timestamp":1788203602512,"version":"build-2803163510"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100011093","name":"University of Phayao","doi-asserted-by":"publisher","award":["30\/2569"],"award-info":[{"award-number":["30\/2569"]}],"id":[{"id":"10.13039\/501100011093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>No evidence-based preanalytical guideline exists for EDTA blood storage before hemoglobin H (HbH) inclusion body detection. We evaluated the stability of rapid (30-minute) brilliant cresyl blue staining across \u03b1-globin and \u03b2-globin genotypes in blood stored at 4\u2009\u00b0C.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Residual EDTA blood from 322 patients with microcytic hypochromic anemia was stored at 4\u2009\u00b0C and tested on days 0, 2, 4, and 7 by 3 blinded medical technologists. Hemoglobin typing and genotyping followed inclusion body counting. Wilcoxon and Mann-Whitney U tests as well as intraclass correlation coefficient(2,1) were applied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Six \u03b1-globin genotype groups were identified, including HbH disease (n\u2009=\u2009103), by \u03b2-globin genotype background. Non-HbH groups showed no meaningful inclusion bodies, confirming stable specificity. In HbH disease with a normal \u03b2-globin gene, 100% qualitative concordance was maintained at day 7, with no statistically significant quantitative change from day 0 (P\u2009=\u2009.28); the intraclass correlation coefficient(2,1) was 0.858. A transient increase at day 2 (P\u2009&amp;lt;\u2009.001) may have reflected in vitro HbH autoxidation during cold storage. Hemoglobin H disease co-inheriting HbE showed stable but markedly reduced counts throughout storage; a consistent pattern was observed in isolated cases co-inheriting \u03b2-thalassemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>EDTA blood stored at 4\u2009\u00b0C maintains qualitative stability for HbH inclusion body detection for up to 7\u2009days; HbE co-inheritance substantially reduces diagnostic sensitivity, with a similar pattern observed in isolated \u03b2-thalassemia co-inheritance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag057","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T11:12:54Z","timestamp":1786101174000},"source":"Crossref","is-referenced-by-count":0,"title":["Preanalytical stability of hemoglobin H inclusion body detection using rapid brilliant cresyl blue staining: a genotype-stratified stability study in EDTA blood stored at 4\u2009\u00b0C"],"prefix":"10.1093","volume":"57","author":[{"given":"Ekkapong","family":"Boriboonwong","sequence":"first","affiliation":[{"name":"Division of Clinical Hematology and Microscopy, Department of Medical Technology, School of Allied Health Sciences, University of Phayao , Muang Phayao, Phayao Province,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanyaphat","family":"Lengkhanyuen","sequence":"additional","affiliation":[{"name":"Division of Clinical Hematology and Microscopy, Department of Medical Technology, School of Allied Health Sciences, University of Phayao , Muang Phayao, Phayao Province,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonchita","family":"Kaewjaem","sequence":"additional","affiliation":[{"name":"Division of Clinical Hematology and Microscopy, Department of Medical Technology, School of Allied Health Sciences, 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