{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:06:42Z","timestamp":1788887202945,"version":"build-2803163510"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Diagnostic significance of calprotectin in the early recognition of inflammatory conditions"},{"name":"the European Union from the European Fund for Regional Development"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Serum calprotectin is an emerging inflammatory biomarker, but data on its stability remain limited. This study aimed to determine the 5-day stability of serum calprotectin stored at 2 temperatures: \u201220\u2009\u00b0C and \u201280\u2009\u00b0C.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study followed Checklist for Reporting Stability Studies recommendations using leftover serum samples. Analysis was undertaken using 2 analytical methods on an automated Siemens Atellica Solution analyzer. Twenty samples in \u201220\u2009\u00b0C storage were analyzed using fCAL turbo reagent (B\u00dcHLMANN), and another 10 samples in \u201220\u2009\u00b0C storage were analyzed using GCAL reagent (Gentian Diagnostics). A further 10 patient samples stored at \u201280\u2009\u00b0C were analyzed using fCAL turbo reagent. The maximum permissible difference was defined as total error, which was 56.43% for fCAL turbo reagent and 46.44% for GCAL reagent.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The instability equations were y\u2009=\u20097.6695\u2009\u00d7\u2009time (days) and y\u2009=\u20090.8553\u2009\u00d7\u2009time (days) for temperatures of \u201220\u2009\u00b0C and \u201280\u2009\u00b0C, respectively, when calprotectin was determined using fCAL turbo reagent and y\u2009=\u200912.7011\u2009\u00d7\u2009time (days) at \u201220\u2009\u00b0C using GCAL reagent.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Results from samples stored for a maximum of 5 days at \u201280\u2009\u00b0C are more reliable than those stored at \u201220\u2009\u00b0C, which may have an impact on clinical interpretation of the results.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag059","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:22:17Z","timestamp":1786706537000},"source":"Crossref","is-referenced-by-count":0,"title":["Stability of serum calprotectin as an emerging inflammatory biomarker: a five-day study at \u221220\u2009\u00b0C and \u221280\u2009\u00b0C using two analytical methods"],"prefix":"10.1093","volume":"57","author":[{"given":"Helena","family":"\u010ci\u010dak","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"},{"name":"Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u017deljka","family":"\u0160ar\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Sara\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lora","family":"Duki\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Diagnostics, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edvard","family":"Gali\u0107","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"},{"name":"School of Medicine, University of Zagreb , Zagreb,","place":["Croatia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Alfirevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Surgery, University Hospital \u201cSveti Duh\u201d, Zagreb, Croatia"},{"name":"Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia"},{"name":"University North, Varazdin, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026090812104727600_lmag059-B1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1177\/0004563219867989","article-title":"The preanalytical phase\u2014past, present and future","volume":"57","author":"Cornes","year":"2020","journal-title":"Ann Clin Biochem"},{"key":"2026090812104727600_lmag059-B2","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1515\/almed-2021-0044","article-title":"Management of post-analytical processes in the clinical laboratory according to ISO 15189:2012. 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