{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T07:01:26Z","timestamp":1789887686802,"version":"4.0.1"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>EDTA-dependent pseudothrombocytopenia (PTP) may cause spuriously low platelet counts and can be prevented by using alternative anticoagulants, such as sodium citrate (citrate) and magnesium sulphate (MgSO4). However, these anticoagulants may affect platelet parameters depending on the analytical platform. Their effect on platelet measurements using the Abbott Alinity hq hematology analyzer has not previously been evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples from 40 outpatients without known PTP were collected in K2-EDTA, citrate, and MgSO4 tubes. Platelet count, mean platelet volume, and reticulated platelet fraction were measured on an Alinity hq analyzer. Paired comparisons were performed using Friedman repeated-measures analysis with the Dunn multiple comparisons test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with EDTA, median platelet counts were statistically significantly lower in citrate (\u221221.1%) and MgSO4 (\u221231.7%) than in EDTA (P\u2009&amp;lt;\u2009.05). Mean platelet volume was also statistically significantly reduced in citrate (\u22123.2%) and MgSO4 (\u22124.7%) (P\u2009&amp;lt;\u2009.05). Median reticulated platelet fraction was slightly higher in both anticoagulants, but only MgSO4 differed substantially from EDTA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>On the Alinity hq analyzer, citrate and MgSO4 result in statistically significantly lower platelet count than EDTA in samples from individuals without known PTP. Platelet parameters are therefore influenced by anticoagulant choice and should not be interpreted interchangeably.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag069","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:20:43Z","timestamp":1788520843000},"source":"Crossref","is-referenced-by-count":0,"title":["Effect of sodium citrate and magnesium sulphate anticoagulation on platelet parameters measured on the Alinity hq analyzer"],"prefix":"10.1093","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5429-3548","authenticated-orcid":false,"given":"Cristine","family":"Betzer","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, G\u00f8dstrup Hospital , Herning,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"McGrail","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, G\u00f8dstrup Hospital , Herning,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"2026092002191961600_lmag069-B1","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1515\/cclm-2012-0081","article-title":"EDTA-dependent pseudothrombocytopenia: further insights and recommendations for prevention of a clinically threatening artifact","volume":"50","author":"Lippi","year":"2012","journal-title":"Clin Chem Lab Med"},{"key":"2026092002191961600_lmag069-B2","first-page":"504","volume-title":"Blood Cells: A Practical Guide","author":"Bain","year":"2015","edition":"5 ed."},{"key":"2026092002191961600_lmag069-B3","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1002\/ajh.2830500206","article-title":"EDTA-dependent pseudothrombocytopenia: a clinical and epidemiological study of 112 cases, with 10-year follow-up","volume":"50","author":"Bizzaro","year":"1995","journal-title":"Am J Hematol"},{"key":"2026092002191961600_lmag069-B4","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1515\/CCLM.2011.013","article-title":"Prevalence and course of pseudothrombocytopenia in outpatients","volume":"49","author":"Froom","year":"2011","journal-title":"Clin Chem Lab Med"},{"key":"2026092002191961600_lmag069-B5","doi-asserted-by":"publisher","first-page":"629","DOI":"10.3109\/00365519709055287","article-title":"Screening for EDTA-dependent deviations in platelet counts and abnormalities in platelet distribution histograms in pseudothrombocytopenia","volume":"57","author":"Bartels","year":"1997","journal-title":"Scand J Clin Lab Invest"},{"key":"2026092002191961600_lmag069-B6","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/1472-6890-14-19","article-title":"A case of EDTA-dependent pseudothrombocytopenia: simple recognition of an underdiagnosed and misleading phenomenon","volume":"14","author":"Nagler","year":"2014","journal-title":"BMC Clin Pathol"},{"key":"2026092002191961600_lmag069-B7","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1111\/ijlh.13966","article-title":"Comparison of platelet count results on the Sysmex XN between citrate or MgSO(4) and K2 EDTA anticoagulants","volume":"45","author":"Soulard","year":"2023","journal-title":"Int J Lab Hematol"},{"key":"2026092002191961600_lmag069-B8","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1093\/ajcp\/aqw066","article-title":"Magnesium sulfate as an alternative in vitro anticoagulant for the measurement of platelet parameters?","volume":"145","author":"Mannu\u00df","year":"2016","journal-title":"Am J Clin Pathol"},{"key":"2026092002191961600_lmag069-B9","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1093\/ajcp\/aqw146","article-title":"Measurement of platelet counts and volume using magnesium sulfate as an anticoagulant: comparison of impedance and light-scatter technology","volume":"146","author":"Mannu\u00df","year":"2016","journal-title":"Am J Clin Pathol"},{"key":"2026092002191961600_lmag069-B10","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9030808","article-title":"Platelet counting: ugly traps and good advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)","volume":"9","author":"Baccini","year":"2020","journal-title":"J Clin Med"},{"key":"2026092002191961600_lmag069-B11","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1111\/ijlh.14430","article-title":"Influence of anticoagulants on platelet counts: A Study and Recommendations From the French Speaking Cellular Hematology Group (GFHC)","volume":"47","author":"Wuilleme","year":"2025","journal-title":"Int J Lab Hematol"},{"key":"2026092002191961600_lmag069-B12","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1111\/ijlh.13082","article-title":"Performance evaluation of new Abbott Alinity hq hematology analyzer","volume":"41","author":"Woo","year":"2019","journal-title":"Int J Lab Hematol"},{"key":"2026092002191961600_lmag069-B13","doi-asserted-by":"publisher","first-page":"1179545X17705380","DOI":"10.1177\/1179545X17705380","article-title":"Estimation of platelet counts and other hematological parameters in pseudothrombocytopenia using alternative anticoagulant: magnesium sulfate","volume":"10","author":"Choccalingam","year":"2017","journal-title":"Clin Med Insights Blood Disord"},{"key":"2026092002191961600_lmag069-B14","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1002\/ajh.23216","article-title":"The platelet count in EDTA-anticoagulated blood from patients with thrombocytopenia may be underestimated when measured in routine laboratories","volume":"87","author":"Podda","year":"2012","journal-title":"Am J Hematol"},{"key":"2026092002191961600_lmag069-B15","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1080\/09537104.2020.1760231","article-title":"Transient appearance of EDTA dependent pseudothrombocytopenia in a patient with 2019 novel coronavirus pneumonia","volume":"31","author":"Li","year":"2020","journal-title":"Platelets"},{"key":"2026092002191961600_lmag069-B16","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1007\/s001340050050","article-title":"Transient EDTA-dependent pseudothrombocytopenia in a patient with sepsis","volume":"26","author":"Mori","year":"2000","journal-title":"Intensive Care Med"}],"container-title":["Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/57\/5\/lmag069\/71236793\/lmag069.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/labmed\/article-pdf\/57\/5\/lmag069\/71236793\/lmag069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:19:22Z","timestamp":1789885162000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/labmed\/article\/doi\/10.1093\/labmed\/lmag069\/8818191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,8,4]]}},"URL":"https:\/\/doi.org\/10.1093\/labmed\/lmag069","relation":{},"ISSN":["0007-5027","1943-7730"],"issn-type":[{"value":"0007-5027","type":"print"},{"value":"1943-7730","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,4]]},"article-number":"lmag069"}}