{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:56:34Z","timestamp":1791305794991,"version":"4.3.1"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>We sought to clarify the interference of red blood cell (RBC) and platelet (PLT) parameters on PLT count by impedance method results and establish an intelligent reflex fluorescence PLT count (PLT-F) confirmation model using PLT-F as the reference to quantify the model\u2019s clinical efficacy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A retrospective analysis was performed on routine blood data from 11\u2009283 patients (June 2023 to February 2024) at our hospital. Patients were stratified into a training set (n\u2009=\u20097898) and verification set (n\u2009=\u20093385) using Laboman AI, version 1.0-312, software (Shanghai Hyson Meikang Medical Electronics Co Ltd). A decision tree algorithm was employed to construct a new model that integrates RBC and PLT parameters for reflex PLT-F confirmation, and the model\u2019s effectiveness was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The combined 3-factor model achieved optimal performance for PLT-F reflex confirmation. Its overall false-negative proportion, release rate, total effective rate, and negative predictive value reached 2.9%, 55.2%, 71.1%, and 94.7%, respectively, in the training set, with matching values of 4.2%, 52.4%, 66.1%, and 91.9%, respectively, in the validation set.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>This study established a novel reflex PLT-F confirmation model based on RBC and PLT parameters. The model achieved a 52.4% sample release rate, with a corresponding overall false-negative proportion of 4.2% in the validation set, presenting a clear trade-off between laboratory operational efficiency and diagnostic safety.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/labmed\/lmag070","type":"journal-article","created":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T11:20:51Z","timestamp":1788693651000},"source":"Crossref","is-referenced-by-count":0,"title":["Construction of a new reflex fluorescence platelet count confirmation model based on red blood cell and platelet parameters"],"prefix":"10.1093","volume":"57","author":[{"given":"Wenjun","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Daping Hospital, Army Medical University , Chongqing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Academy of Military Medical Sciences , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Daping Hospital, Army Medical University , Chongqing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Daping Hospital, Army Medical University , Chongqing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoli","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Daping Hospital, Army Medical University , Chongqing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Daping Hospital, Army Medical University , Chongqing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"2026100612332472200_lmag070-B1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.5858\/arpa.2016-0222-OA","article-title":"Performance evaluation of automated impedance and optical fluorescence platelet counts compared with international reference method in patients with thalassemia","volume":"141","author":"Tantanate","year":"2017","journal-title":"Arch Pathol Lab Med"},{"key":"2026100612332472200_lmag070-B2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/ijlh.12503","article-title":"Verification and quality control of routine hematology analyzers","volume":"38 Suppl 1","author":"Vis","year":"2016","journal-title":"Int J Lab Hematol"},{"key":"2026100612332472200_lmag070-B3","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1309\/AJCPUAGGB4URL5XO","article-title":"New fluorescent method (PLT-F) on Sysmex XN2000 hematology analyzer achieved higher accuracy in low platelet counting","volume":"140","author":"Schoorl","year":"2013","journal-title":"Am J Clin Pathol"},{"key":"2026100612332472200_lmag070-B4","doi-asserted-by":"crossref","first-page":"131","DOI":"10.4103\/ajts.ajts_65_21","article-title":"Platelet count in impedance-based hematology analyzer: Beware of trap!","volume":"17","author":"Krishnamurthy","year":"2023","journal-title":"Asian J Transfus Sci"},{"key":"2026100612332472200_lmag070-B5","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1159\/000346434","article-title":"Pseudothrombocytosis due to microerythrocytosis: a case of beta thalassemia minor complicated with iron deficiency anemia","volume":"130","author":"Akinci","year":"2013","journal-title":"Acta Haematol"},{"key":"2026100612332472200_lmag070-B6","doi-asserted-by":"crossref","first-page":"e463","DOI":"10.18295\/squmj.2015.15.04.004","article-title":"Accuracy of platelet counting by optical and impedance methods in patients with thrombocytopaenia and microcytosis","volume":"15","author":"Boulassel","year":"2015","journal-title":"Sultan Qaboos Univ Med J"},{"key":"2026100612332472200_lmag070-B7","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/j.1365-2257.2006.00870.x","article-title":"Spurious counts and spurious results on haematology analysers: a review. 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