{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:20:09Z","timestamp":1648675209355},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Several Kell-system antibodies are known to cause direct agglutination. Also, some specificities, such as anti-Ku, have been reported to react only via the indirect antiglobulin test (IAT).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Herein, we describe the case of a 61-year-old alloimmunized white woman who presented to an outside hospital with a gastrointestinal (GI) bleed and a \u201cpossible anti-Ku\u201d was reported with 3+ reactivity at PEG-IAT and at Ficin-IAT; in addition to an unidentified cold antibody. Subsequently, when the patient presented to a second outside hospital, an anti-Ku that caused 3+ to 4+ reactions at saline-immediate spin (IS) was identified. The reactivity was evaluated with 0.01-M dithiothreitol (DTT) treatment of the plasma.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>It was determined that the strong agglutination with saline-IS was caused by immunoglobulin (Ig)M anti-Ku.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>To our knowledge, this is the first reported case of an IgM anti-Ku.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmz021","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T23:13:41Z","timestamp":1554765221000},"page":"47-49","source":"Crossref","is-referenced-by-count":0,"title":["Novel Example of a Direct-Agglutinating Anti-Ku"],"prefix":"10.1093","volume":"51","author":[{"given":"Michael Sel","family":"Gannett","sequence":"first","affiliation":[{"name":"Immunohematology Reference Laboratory\u2014Orlando, Orlando, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard R","family":"Gammon","sequence":"additional","affiliation":[{"name":"Scientific Medical and Technical Administration, OneBlood, Inc, Orlando, Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,5,30]]},"reference":[{"issue":"3","key":"2020010600275409400_CIT0001","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/vox.12628","article-title":"Silent KEL alleles identified from Japanese individuals with the Ko phenotype","volume":"113","author":"Onodera","year":"2018","journal-title":"Vox Sang."},{"issue":"4588","key":"2020010600275409400_CIT0002","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/180711a0","article-title":"A new Kell blood-group phenotype","volume":"180","author":"Chown","year":"1957","journal-title":"Nature."},{"key":"2020010600275409400_CIT0003","first-page":"437","article-title":"Two K0 rare blood group families found in donors [in Japanese]","volume":"5","author":"Okubo","year":"1982","journal-title":"J Soc Jpn Blood Programme."},{"issue":"5","key":"2020010600275409400_CIT0004","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1365-3148.2009.00962.x","article-title":"Two novel null alleles of the KEL gene detected in two Chinese women with the Knull phenotype","volume":"19","author":"Yang","year":"2009","journal-title":"Transfus Med."},{"key":"2020010600275409400_CIT0005","volume-title":"Blood Groups in Man","author":"Race","year":"1975","edition":"6"},{"key":"2020010600275409400_CIT0006","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1002\/9781118493595","volume-title":"Human Blood Groups","author":"Daniels","year":"2013","edition":"3"},{"key":"2020010600275409400_CIT0007","first-page":"301","volume-title":"The Blood Group Antigen FactsBook","author":"Reid","year":"2012","edition":"3"},{"key":"2020010600275409400_CIT0008","article-title":"Other blood group system and antigens.","volume-title":"Technical Manual","author":"Storry","year":"2017","edition":"19"},{"issue":"5","key":"2020010600275409400_CIT0009","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1159\/000466780","article-title":"DTT test: a new method to differentiate IgM and IgG erythrocyte antibodies","volume":"27","author":"Pirofsky","year":"1974","journal-title":"Vox Sang."},{"issue":"2","key":"2020010600275409400_CIT0010","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1159\/000465546","article-title":"A simple technique for the inactivation of IgM antibodies using dithiothreitol","volume":"30","author":"Olson","year":"1976","journal-title":"Vox Sang."},{"key":"2020010600275409400_CIT0011","first-page":"55","volume-title":"Applied Blood Group Serology","author":"Issitt","year":"1998","edition":"4"}],"container-title":["Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/labmed\/article-pdf\/51\/1\/47\/31712656\/lmz021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/labmed\/article-pdf\/51\/1\/47\/31712656\/lmz021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T00:27:58Z","timestamp":1578270478000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/labmed\/article\/51\/1\/47\/5506496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,30]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,5,30]]},"published-print":{"date-parts":[[2020,1,2]]}},"URL":"https:\/\/doi.org\/10.1093\/labmed\/lmz021","relation":{},"ISSN":["0007-5027","1943-7730"],"issn-type":[{"value":"0007-5027","type":"print"},{"value":"1943-7730","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,1]]},"published":{"date-parts":[[2019,5,30]]}}}