{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:41:00Z","timestamp":1785894060296,"version":"3.56.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T00:00:00Z","timestamp":1557273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The Verigene Enteric Pathogens Test (Luminex Corporation) is a polymerase chain reaction (PCR)\/nucleic acid microarray-based assay targeting 8 bacterial and viral pathogens that cause diarrhea.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To compare traditional enteric culture methods with stool testing by Verigene EP (PCR\/microarray).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Tests were performed using PCR\/microarray between February and August 2016. All specimens also underwent culture for Salmonella and Shigella; specimens that tested positive for bacterial pathogen(s) had confirmatory cultures.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Valid results were obtained for 99.3% of the 3795 stool specimens. Among these, 497 (13.2%) specimens tested positive for at least 1 pathogen by PCR\/microarray; 45.5% of these tested positive for 1 or more bacterial pathogens. Agreement between positive bacterial PCR\/microarray results and culture-based testing was 85.3%. Compared with cultures, PCR\/microarray demonstrated 95.2% and 87.5% sensitivity and 99.8% and 99.8% specificity for Salmonella and Shigella, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The Verigene EP generated evaluable results for most stool specimens tested and demonstrated good agreement with bacterial cultures.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/labmed\/lmz022","type":"journal-article","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T16:12:49Z","timestamp":1553184769000},"page":"390-395","source":"Crossref","is-referenced-by-count":8,"title":["Prospective Investigation of an Automated PCR\/Nucleic Acid Microarray-Based Platform for Enteric Pathogen Testing"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7127-6889","authenticated-orcid":false,"given":"Amy K","family":"Beckman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Ferrieri","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"issue":"1","key":"2019101009585942000_CIT0001","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1097\/MCG.0b013e31826df662","article-title":"Acute diarrhea in adults and children: a global perspective","volume":"47","author":"Farthing","year":"2013","journal-title":"J Clin Gastroenterol."},{"issue":"16","key":"2019101009585942000_CIT0002","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1056\/NEJMra1301069","article-title":"Acute infectious diarrhea in immunocompetent adults","volume":"370","author":"DuPont","year":"2014","journal-title":"N Engl J Med."},{"issue":"1","key":"2019101009585942000_CIT0003","doi-asserted-by":"crossref","first-page":"16","DOI":"10.3201\/eid1701.P21101","article-title":"Foodborne illness acquired in the United States\u2013unspecified agents","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerg Infect Dis."},{"issue":"1","key":"2019101009585942000_CIT0004","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne illness acquired in the United States\u2013major pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerg Infect Dis."},{"issue":"5","key":"2019101009585942000_CIT0005","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/ajg.2016.126","article-title":"ACG clinical guideline: diagnosis, treatment, and prevention of acute diarrheal infections in adults","volume":"111","author":"Riddle","year":"2016","journal-title":"Am J Gastroenterol."},{"issue":"4","key":"2019101009585942000_CIT0006","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.diagmicrobio.2016.09.013","article-title":"Performance of the Verigene\u00ae enteric pathogens test, Biofire FilmArray\u2122 gastrointestinal panel and Luminex xTAG\u00ae gastrointestinal pathogen panel for detection of common enteric pathogens","volume":"86","author":"Huang","year":"2016","journal-title":"Diagn Microbiol Infect Dis."},{"issue":"3","key":"2019101009585942000_CIT0007","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1086\/318514","article-title":"Practice guidelines for the management of infectious diarrhea","volume":"32","author":"Guerrant","year":"2001","journal-title":"Clin Infect Dis."},{"key":"2019101009585942000_CIT0008","first-page":"e00024-17","article-title":"Syndromic panel-based testing in clinical microbiology","volume-title":"Clin Microbiol Rev","author":"Ramanan","year":"2017"},{"issue":"10","key":"2019101009585942000_CIT0009","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1093\/cid\/ciw494","article-title":"Multiplexed molecular diagnostics for respiratory, gastrointestinal, and central nervous system infections","volume":"63","author":"Hanson","year":"2016","journal-title":"Clin Infect Dis."},{"key":"2019101009585942000_CIT0010","volume-title":"510(k) Premarket Notification (Substantial Equivalence Determination Decision Summary for Nanosphere, Inc. 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