{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:23:08Z","timestamp":1782271388610,"version":"3.54.5"},"reference-count":37,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T00:00:00Z","timestamp":1573430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Encephalitis is caused by infection, immune mediated diseases, or primary inflammatory diseases. Of all the causative infectious pathogens, 90% are viruses or bacteria. Granulomatous amoebic encephalitis (GAE), caused by Balamuthia mandrillaris, is a rare but life-threatening disease. Diagnosis and therapy are frequently delayed due to the lack of specific clinical manifestations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>A healthy 2 year old Chinese male patient initially presented with a nearly 2 month history of irregular fever. We present this case of granulomatous amoebic encephalitis caused by B. mandrillaris. Next generation sequencing of the patient\u2019s cerebrospinal fluid (CSF) was performed to identify an infectious agent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>The results of next generation sequencing of the CSF showed that most of the mapped reads belonged to Balamuthia mandrillaris.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Next generation sequencing (NGS) is an unbiased and rapid diagnostic tool. The NGS method can be used for the rapid identification of causative pathogens. The NGS method should be widely applied in clinical practice and help clinicians provide direction for the diagnosis of diseases, especially for rare and difficult cases.<\/jats:p><\/jats:sec>","DOI":"10.1093\/labmed\/lmz079","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T11:25:37Z","timestamp":1569497137000},"source":"Crossref","is-referenced-by-count":19,"title":["Balamuthia mandrillaris-Related Primary Amoebic Encephalitis in China Diagnosed by Next Generation Sequencing and a Review of the Literature"],"prefix":"10.1093","author":[{"given":"Yinan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Lanzhou University Second Hospital, Lanzhou, Gansu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobin","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Public Health, Lanzhou University, Cheng Guan District, Lanzhou, Gansu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Lanzhou University Second Hospital, Lanzhou, Gansu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Lanzhou University Second Hospital, Lanzhou, Gansu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanlin","family":"Guan","sequence":"additional","affiliation":[{"name":"Chief Information Officer, Hugobiotech MicrobeCode Biotechnology Co. 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