{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T23:44:07Z","timestamp":1746056647142,"version":"3.37.3"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Automated systems for antinuclear antibody (ANA) testing provide endpoint titers that are predicted based on the fluorescence intensity (FI) value at a screening dilution (single-well titration [SWT]) showing frequent titration errors (more than plus or minus 1 dilution).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Line slope titration (LST) was based on the trend of FI values on dilutions. Three dilutions per specimen were prepared considering a patient\u2019s previous titer or FI at the screening dilution. On the XY plot, with the reciprocal of dilution as the X-axis and FI value as the Y-axis, a fitted line was drawn to obtain the endpoint titers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The titration error rate (no. of errors\/total no.) of LST using a regression line was lower than that of SWT (31\/710 [4.4%] and 152\/674 [22.6%], respectively; P &amp;lt; .000000001), with serial dilution as a reference. When comparing a regression line using 3 dilution points with a line using 2 dilution points, the error rate of the former was not significantly different from that of the latter (31\/710 [4.4%] and 31\/746 [4.2%], respectively; P = .842).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This LST method is useful as an accurate, cost-effective, and rapid approach to measure endpoint titers in routine ANA testing.<\/jats:p><\/jats:sec>","DOI":"10.1093\/labmed\/lmz087","type":"journal-article","created":{"date-parts":[[2019,10,19]],"date-time":"2019-10-19T19:08:24Z","timestamp":1571512104000},"page":"469-477","source":"Crossref","is-referenced-by-count":5,"title":["Measurements of Endpoint Titers Based on the Fluorescence Intensity Trend in Anti-Nuclear Antibody Testing"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1983-2515","authenticated-orcid":false,"given":"Dong I L","family":"Won","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,12,24]]},"reference":[{"issue":"2","key":"2020090103121736100_CIT0001","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3109\/08916938909019953","article-title":"Antinuclear antibodies detected by indirect immunofluorescence on HEp2 cells and by immunoblotting in patients with systemic sclerosis","volume":"2","author":"Briolay","year":"1989","journal-title":"Autoimmunity."},{"key":"2020090103121736100_CIT0002","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.rinim.2013.10.003","article-title":"Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: analytical study in a specialized health institute, one year 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