{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:06:02Z","timestamp":1753877162813,"version":"3.41.2"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases (AmpCs)-producing Enterobacteriaceae have been increasingly reported and imposing significant threat to public. Livestock production industry might be the important source for clinically important ESBL-producing Enterobacteriaceae. This study aims to investigate the resistance profile, phenotypic ESBL production, beta-lactamase genes, virulence factors, and plasmid replicon types among 59 Enterobacteriaceae strains isolated from poultry faecal samples in Malaysia\u2019s commercial poultry farm. There were 38.7% and 32.3% of Escherichia coli resistant to cefotaxime and cefoxitin, respectively, while Klebsiellaspp. demonstrated resistance rate of 52.6% to both mentioned antimicrobials. Majority of the E. coli isolates carried blaTEM and blaCMY-2 group. blaSHV was the most prevalent gene detected in Klebsiellaspp., followed by blaDHA and blaTEM. Resistance to extended spectrum cephalosporin in our isolates was primarily mediated by plasmid mediated AmpC beta-lactamase such as CMY-2 group and DHA enzyme. The CTX-M genes were found in two ESBL-producing E. coli. IncF, IncI1, and IncN plasmids were most frequently detected in E. coli and Klebsiellaspp. The virulence factor, including EAST1 and pAA were identified at low frequency. This study highlights the poultry as a reservoir of resistance and virulence determinants and prevalence of plasmids in Enterobacteriaceae might drive their dissemination.<\/jats:p>","DOI":"10.1093\/lambio\/ovac044","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T13:43:34Z","timestamp":1712583814000},"source":"Crossref","is-referenced-by-count":5,"title":["Characterisation of ESBL\/AmpC-Producing <i>Enterobacteriaceae<\/i> isolated from poultry farms in Peninsular Malaysia"],"prefix":"10.1093","volume":"76","author":[{"given":"Hui-Shee","family":"Tan","sequence":"first","affiliation":[{"name":"Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia , 43500 Semenyih, Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Yan","sequence":"additional","affiliation":[{"name":"Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia , 43500 Semenyih, Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hollysia Alda","family":"Agustie","sequence":"additional","affiliation":[{"name":"Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia , 43500 Semenyih, Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwei-San","family":"Loh","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Nottingham Malaysia , 43500 Semenyih, Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabin","family":"Rayamajhi","sequence":"additional","affiliation":[{"name":"Warrenton Regional Animal Health Laboratory , 272 Academy Hill Rd, Warrenton, VA 20186 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chee-Mun","family":"Fang","sequence":"additional","affiliation":[{"name":"Division of Biomedical Sciences, School of Pharmacy, University of Nottingham Malaysia , 43500 Semenyih, Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"2024040813410653300_bib1","first-page":"29","article-title":"Coexistence of plasmid-mediated quinolone resistance determinants and AmpC-Beta-Lactamases in Escherichia coli strains in Egypt","volume":"61","author":"Abd\u00a0El-Aziz","year":"2015","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"2024040813410653300_bib2","doi-asserted-by":"crossref","first-page":"4568","DOI":"10.1128\/JB.186.14.4568-4574.2004","article-title":"Transposition of the heat-stable toxin astA gene into a gifsy-2-related prophage of Salmonella enterica serovar Abortusovis","volume":"186","author":"Bacciu","year":"2004","journal-title":"J Bacteriol"},{"key":"2024040813410653300_bib3","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1128\/CMR.00059-12","article-title":"Antimicrobial resistance and virulence: a successful or deleterious association in the bacterial world?","volume":"26","author":"Beceiro","year":"2013","journal-title":"Clin Microbiol Rev"},{"key":"2024040813410653300_bib4","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1093\/jac\/dkx146","article-title":"Global epidemiology of CTX-M \u03b2-lactamases: temporal and geographical shifts in genotype","volume":"72","author":"Bevan","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2024040813410653300_bib5","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1179\/2047773214Y.0000000148","article-title":"Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand","volume":"108","author":"Boonyasiri","year":"2014","journal-title":"Pathog Glob Health"},{"key":"2024040813410653300_bib6","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing","volume":"63","author":"Carattoli","year":"2005","journal-title":"J Microbiol Methods"},{"volume-title":"Performance Standards for Antimicrobial Susceptibility Tests. 31st ed. 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