{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:07:45Z","timestamp":1791270465222,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01DE027669"],"award-info":[{"award-number":["R01DE027669"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The uptake and storage of extracellular orthophosphate (Pi) by polyphosphate (polyP) accumulating bacteria may contribute to mineral dissolution in the oral cavity. To test the effect of potential inhibitors of polyP kinases on Rothia dentocariosa, gallein (0, 25, 50, and 100\u2009\u03bcM) and fluoride (0, 50, and 100\u2009ppm) were added to R. dentocariosa cultures grown in brain-heart infusion broth. At a late log growth phase (8\u2009h), extracellular Pi was measured using an ascorbic acid assay, and polyP was isolated from bacterial cells treated with RNA\/DNAases using a neutral phenol\/chloroform extraction. Extracts were hydrolyzed and quantified as above. Gallein and fluoride had minor effects on bacterial growth with NaF having a direct effect on media pH. Gallein (\u226525\u2009\u03bcM) and fluoride (\u226550\u2009ppm) attenuated the bacterial drawdown of extracellular Pi by 56.7% (P\u00a0&amp;lt;\u00a00.05) and 37.3% (P\u00a0&amp;lt;\u00a00.01). There was a corresponding polyP synthesis decrease of 73.2% (P\u00a0&amp;lt;\u00a00.0001) from gallein and 83.1% (P\u00a0&amp;lt;\u00a00.0001) from fluoride. Attenuated total reflectance-Fourier-transform infrared spectroscopy validated the presence of polyP and its reduced concentration in R. dentocariosa bacterial cells following gallein and fluoride treatment. Rothia dentocariosa can directly change extracellular Pi and accumulate intracellular polyP, but the mechanism is attenuated by gallein and NaF.<\/jats:p>","DOI":"10.1093\/lambio\/ovad017","type":"journal-article","created":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T02:30:03Z","timestamp":1674873003000},"source":"Crossref","is-referenced-by-count":10,"title":["Fluoride and gallein inhibit polyphosphate accumulation by oral pathogen\n                    <i>Rothia dentocariosa<\/i>"],"prefix":"10.1093","volume":"76","author":[{"given":"Dhiraj","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Developmental and Surgical Sciences, University of Minnesota , Minneapolis, MN 55455 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhrangshu","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, University of Minnesota , Minneapolis, MN 55455 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake V","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, University of Minnesota , Minneapolis, MN 55455 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly E","family":"Flood","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, University of Minnesota , Minneapolis, MN 55455 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9213-3657","authenticated-orcid":false,"given":"Robert S","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Developmental and Surgical Sciences, University of Minnesota , Minneapolis, MN 55455 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,1,28]]},"reference":[{"key":"2024040813211946100_bib1","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1128\/JCM.01410-07","article-title":"Bacteria of dental caries in primary and permanent teeth in children and young adults","volume":"46","author":"Aas","year":"2008","journal-title":"J Clin Microbiol"},{"key":"2024040813211946100_bib2","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the normal bacterial flora of the oral cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J Clin Microbiol"},{"key":"2024040813211946100_bib3","doi-asserted-by":"crossref","first-page":"1557986","DOI":"10.1080\/20002297.2018.1557986","article-title":"Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children","volume":"11","author":"Al-Hebshi","year":"2019","journal-title":"J Oral Microbiol"},{"key":"2024040813211946100_bib4","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1038\/nature05457","article-title":"Evidence of giant sulphur bacteria in Neoproterozoic phosphorites","volume":"445","author":"Bailey","year":"2006","journal-title":"Nature"},{"key":"2024040813211946100_bib5","doi-asserted-by":"crossref","first-page":"e02440","DOI":"10.1128\/AEM.02440-17","article-title":"Polyphosphate-accumulating bacteria: potential contributors to mineral dissolution in the oral cavity","volume":"84","author":"Breiland","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"2024040813211946100_bib6","doi-asserted-by":"crossref","first-page":"6","DOI":"10.15698\/mic2017.01.551","article-title":"Improvement of biochemical methods of polyP quantification","volume":"4","author":"Bru","year":"2016","journal-title":"Microbial Cell"},{"key":"2024040813211946100_bib7","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s12866-020-01801-y","article-title":"Defining the oral microbiome by whole-genome sequencing and resistome analysis: the complexity of the healthy picture","volume":"20","author":"Caselli","year":"2020","journal-title":"BMC Microbiol"},{"key":"2024040813211946100_bib8","doi-asserted-by":"crossref","first-page":"D507","DOI":"10.1093\/nar\/gkw929","article-title":"IMG\/M: integrated genome and metagenome comparative data analysis system","volume":"45","author":"Chen","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"2024040813211946100_bib9","doi-asserted-by":"crossref","first-page":"4167","DOI":"10.1021\/acs.analchem.9b05144","article-title":"Methods for the analysis of polyphosphate in the life sciences","volume":"92","author":"Christ","year":"2020","journal-title":"Anal Chem"},{"key":"2024040813211946100_bib10","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"the human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"2024040813211946100_bib11","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1128\/AEM.72.4.2837-2848.2006","article-title":"Molecular characterization of subject-specific oral microflora during initial colonization of enamel","volume":"72","author":"Diaz","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"2024040813211946100_bib12","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1128\/jb.154.2.803-808.1983","article-title":"Fluoride, hydrogen, and formate activate ribulosebisphosphate carboxylase formation in Alcaligenes eutrophus","volume":"154","author":"Im","year":"1983","journal-title":"J Bacteriol"},{"key":"2024040813211946100_bib13","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/ngeo913","article-title":"Microbial sequestration of phosphorus in anoxic upwelling sediments","volume":"3","author":"Goldhammer","year":"2010","journal-title":"Nat Geosci"},{"key":"2024040813211946100_bib14","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1177\/0022034520979926","article-title":"Oral microbiota composition predicts early childhood caries onset","volume":"100","author":"Grier","year":"2021","journal-title":"J Dent Res"},{"key":"2024040813211946100_bib15","doi-asserted-by":"crossref","first-page":"e47722","DOI":"10.1371\/journal.pone.0047722","article-title":"Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis","volume":"7","author":"Gross","year":"2012","journal-title":"PLoS One"},{"key":"2024040813211946100_bib16","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s00248-017-1056-1","article-title":"Taxonomic and functional analyses of the supragingival microbiome from caries-affected and caries-free hosts","volume":"75","author":"He","year":"2018","journal-title":"Microb Ecol"},{"key":"2024040813211946100_bib17","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.2136\/sssaj1998.03615995006200060009x","article-title":"Determination of soluble phosphorus in the presence of organic ligands or fluoride","volume":"62","author":"He","year":"1998","journal-title":"Soil Sci Soc Am J"},{"key":"2024040813211946100_bib18","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1128\/AEM.03984-15","article-title":"Microbial diversity in the early in vivo-formed dental biofilm","volume":"82","author":"Heller","year":"2016","journal-title":"Appl Environ Microbiol"},{"key":"2024040813211946100_bib19","doi-asserted-by":"crossref","first-page":"e00360","DOI":"10.1128\/mSystems.00360-19","article-title":"Identification of Initial colonizing bacteria in dental plaques from young adults using full-length 16S rRNA gene sequencing","volume":"4","author":"Ihara","year":"2019","journal-title":"Msystems"},{"key":"2024040813211946100_bib20","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.3390\/ijms17121978","article-title":"Salivary microbiome diversity in caries-free and caries-affected children","volume":"7","author":"Jiang","year":"2016","journal-title":"Int J Mol Sci"},{"key":"2024040813211946100_bib21","doi-asserted-by":"crossref","first-page":"88445","DOI":"10.1039\/C5RA14586D","article-title":"Microencapsulation of ammonium polyphosphate with melamine-formaldehyde-tris(2-hydroxyethyl)isocyanurate resin and its flame retardancy in polypropylene","volume":"5","author":"Jiang","year":"2015","journal-title":"RSC Adv"},{"key":"2024040813211946100_bib22","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1177\/0022034515609554","article-title":"The microbiome in populations with a low and high prevalence of caries","volume":"95","author":"Johansson","year":"2015","journal-title":"J Dent Res"},{"key":"2024040813211946100_bib23","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1159\/000320158","article-title":"Clonal analysis of the microbiota of severe early childhood caries","volume":"44","author":"Kanasi","year":"2010","journal-title":"Caries Res"},{"key":"2024040813211946100_bib24","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1039\/c2an35289c","article-title":"Quantitative determination of polyphosphate in sediments using attenuated total reflectance\u2013Fourier-transform infrared (ATR\u2013FTIR) spectroscopy and partial least squares regression","volume":"137","author":"Khoshmanesh","year":"2012","journal-title":"Analyst"},{"key":"2024040813211946100_bib25","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3389\/fcimb.2015.00010","article-title":"Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms","volume":"5","author":"Klein","year":"2015","journal-title":"Front Cell Infect Microbiol"},{"key":"2024040813211946100_bib26","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/jb.177.3.491-496.1995","article-title":"Inorganic polyphosphate: toward making a forgotten polymer unforgettable","volume":"177","author":"Kornberg","year":"1995","journal-title":"J Bacteriol"},{"key":"2024040813211946100_bib27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0006-3002(56)90280-3","article-title":"Metaphosphate synthesis by an enzyme from Escherichia coli","volume":"20","author":"Kornberg","year":"1956","journal-title":"Biochim Biophys Acta"},{"key":"2024040813211946100_bib28","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1146\/annurev.biochem.68.1.89","article-title":"Inorganic polyphosphate: a molecule of many functions","volume":"68","author":"Kornberg","year":"1999","journal-title":"Annu Rev Biochem"},{"key":"2024040813211946100_bib29","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41579-018-0089-x","article-title":"the oral microbiota: dynamic communities and host interactions","volume":"16","author":"Lamont","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"2024040813211946100_bib30","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1128\/JCM.01622-06","article-title":"Genetic profiling of the oral microbiota associated with severe early-childhood caries","volume":"45","author":"Li","year":"2007","journal-title":"J Clin Microbiol"},{"key":"2024040813211946100_bib31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1159\/000260915","article-title":"Inorganic phosphate assay with the ascorbic acid or stannous chloride method: influence of fluoride","volume":"20","author":"Linden","year":"1986","journal-title":"Caries Res"},{"key":"2024040813211946100_bib32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1099\/mic.0.26082-0","article-title":"Are dental diseases examples of ecological catastrophes?","volume":"149","author":"Marsh","year":"2003","journal-title":"Microbiology"},{"key":"2024040813211946100_bib33","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1016\/S0043-1354(98)00129-8","article-title":"Microbiology and biochemistry of the enhanced biological phosphate removal process","volume":"32","author":"Mino","year":"1998","journal-title":"Water Res"},{"key":"2024040813211946100_bib34","doi-asserted-by":"crossref","first-page":"4821","DOI":"10.1128\/AEM.01205-16","article-title":"Effects of carbohydrate source on genetic competence in Streptococcus mutans","volume":"82","author":"Moye","year":"2016","journal-title":"Appl Environ Microbiol"},{"key":"2024040813211946100_bib35","doi-asserted-by":"crossref","first-page":"e0188977","DOI":"10.1371\/journal.pone.0188977","article-title":"Uptake of polyphosphate microparticles in vitro (SaOS-2 and HUVEC cells) followed by an increase of the intracellular ATP pool size","volume":"12","author":"M\u00fcller","year":"2017","journal-title":"PLoS One"},{"key":"2024040813211946100_bib36","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1128\/JCM.42.7.3023-3029.2004","article-title":"Molecular analysis of the microflora associated with dental caries","volume":"42","author":"Munson","year":"2004","journal-title":"J Clin Microbiol"},{"key":"2024040813211946100_bib37","first-page":"e00592","article-title":"a dual-specificity Inhibitor targets polyphosphate kinase 1 and 2 enzymes to attenuate virulence of Pseudomonas aeruginosa","volume":"12","author":"Nolan","year":"2021","journal-title":"MBio"},{"key":"2024040813211946100_bib38","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/1348-0421.12283","article-title":"Nitrogenous compounds stimulate glucose-derived acid production by oral Streptococcus and Actinomyces","volume":"59","author":"Norimatsu","year":"2015","journal-title":"Microbiol Immunol"},{"key":"2024040813211946100_bib39","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1146\/annurev.biochem.77.083007.093039","article-title":"Inorganic polyphosphate: essential for growth and survival","volume":"78","author":"Rao","year":"2009","journal-title":"Annu Rev Biochem"},{"key":"2024040813211946100_bib40","doi-asserted-by":"crossref","first-page":"e00106","DOI":"10.1128\/IAI.00106-17","article-title":"Microbiomes of site-specific dental plaques from children with different caries status","volume":"85","author":"Richards","year":"2017","journal-title":"Infect Immun"},{"key":"2024040813211946100_bib41","first-page":"764733","article-title":"Broad-spectrum inhibitor of bacterial polyphosphate homeostasis attenuates virulence factors and helps reveal novel physiology of Klebsiella pneumoniae and Acinetobacter baumannii","volume":"12","author":"Roberge","year":"2021","journal-title":"Front\u00a0Microbiol"},{"key":"2024040813211946100_bib42","doi-asserted-by":"crossref","first-page":"e3277","DOI":"10.7717\/peerj.3277","article-title":"Insights of the dental calculi microbiome of pre-Columbian inhabitants from Puerto Rico","volume":"5","author":"Santiago-Rodriguez","year":"2017","journal-title":"PeerJ"},{"key":"2024040813211946100_bib43","doi-asserted-by":"crossref","first-page":"e00847","DOI":"10.1128\/JB.00847-16","article-title":"Pleiotropic regulation of virulence Genes in Streptococcus mutans by the conserved small Protein SprV","volume":"199","author":"Shankar","year":"2017","journal-title":"J Bacteriol"},{"key":"2024040813211946100_bib44","doi-asserted-by":"crossref","first-page":"13955","DOI":"10.1039\/C4TA02778G","article-title":"Ammonium polyphosphate chemically-modified with ethanolamine as an efficient intumescent flame retardant for polypropylene","volume":"2","author":"Shao","year":"2014","journal-title":"J Mater Chem A"},{"key":"2024040813211946100_bib45","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1111\/j.1600-0765.1978.tb00190.x","article-title":"a microbiological study of dental calculus","volume":"13","author":"Sidaway","year":"1978","journal-title":"J Periodontal Res"},{"key":"2024040813211946100_bib46","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1159\/000339482","article-title":"Shifts in the microbial population in relation to in situ caries progression","volume":"46","author":"Thomas","year":"2012","journal-title":"Caries Res"},{"key":"2024040813211946100_bib47","doi-asserted-by":"crossref","first-page":"e00161","DOI":"10.1128\/mSystems.00161-20","article-title":"Commensal oral Rothia mucilaginosa\u00a0produces enterobactin, a metal-chelating siderophore","volume":"5","author":"Uranga","year":"2020","journal-title":"Msystems"},{"key":"2024040813211946100_bib48","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/cre2.206","article-title":"Profiling microorganisms in whole saliva of children with and without dental caries","volume":"5","author":"Vieira","year":"2019","journal-title":"Clin Exp Dent Res"},{"key":"2024040813211946100_bib49","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.3389\/fmicb.2017.02244","article-title":"Profiling of oral microbiota in early childhood caries using single-molecule real-time sequencing","volume":"8","author":"Wang","year":"2017","journal-title":"Front Microbiol"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovad017\/49159240\/ovad017.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/76\/2\/ovad017\/53668969\/ovad017.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/76\/2\/ovad017\/53668969\/ovad017.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T09:21:41Z","timestamp":1712568101000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovad017\/7008850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,28]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2,16]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovad017","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,2]]},"published":{"date-parts":[[2023,1,28]]},"article-number":"ovad017"}}