{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T02:53:52Z","timestamp":1778900032117,"version":"3.51.4"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T00:00:00Z","timestamp":1677283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Spanish Ministry of Defence"},{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coxiella burnetii, the causative agent of Q fever, is a small, coccoid, Gram-negative strict intracellular pathogen. One of the most common ways of acquiring Q fever is through inhalation of aerosols containing the bacteria. Because C. burnetii is highly infectious, spreads easily through the air, and is very resistant to environmental conditions, it is considered a biological threat. This paper presents the development and validation of a specific real-time polymerase chain reaction (real-time PCR or qPCR) assay for the detection of C. burnetii, based on the amplification of a fragment of the isocitrate dehydrogenase (icd) encoding gene. This real-time PCR is highly specific, reproducible, and sensitive, allowing the detection of as few as 5 genome equivalents (GEs) of C. burnetii per reaction. The method enables a rapid preliminary differentiation among strains, based on a point mutation at nucleotide 745 of the icd gene. The assay was successfully evaluated in environmental soil samples; a limit of detection of 3\u00a0\u00d7\u00a0104 colony forming units per 0.5\u00a0g of soil (\u223c3 GEs per reaction) was achieved. The newly developed real-time PCR offers a valuable tool for differential detection of C. burnetii strains in environmental soil samples.<\/jats:p>","DOI":"10.1093\/lambio\/ovad030","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T11:43:07Z","timestamp":1677238987000},"source":"Crossref","is-referenced-by-count":5,"title":["Development of a specific real-time PCR assay for simultaneous detection and differentiation of<i>Coxiella burnetii<\/i>strains from environmental soil samples"],"prefix":"10.1093","volume":"76","author":[{"given":"Juan","family":"Fern\u00e1ndez-Carrillo","sequence":"first","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"del Olmo-Monge","sequence":"additional","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricela E","family":"Sellek","sequence":"additional","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Ortega-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Carlos","family":"Cabria-Ramos","sequence":"additional","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Bassy","sequence":"additional","affiliation":[{"name":"Biological Defence Area, CBRN Defence Systems Department, Campus La Mara\u00f1osa, Instituto Nacional de T\u00e9cnica Aeroespacial (INTA), San Mart\u00edn de la Vega , 28330 Madrid , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,2,25]]},"reference":[{"key":"2023032410082744700_","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search 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