{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T23:00:12Z","timestamp":1776726012617,"version":"3.51.2"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004901","name":"FAPEMIG","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prophylactic and therapeutic overuse of antimicrobials on the farm has contributed to the emergence of hard-to-fight bacterial strains causing bovine mastitis. Aiming at alternative therapies, this study evaluated the antimicrobial activity of 20 essential oils against clinical Staphylococcus aureus strains. Of them, five with strong activities were selected and evaluated for their minimum inhibitory concentrations (MIC) in culture medium and milk, cytotoxicity against bovine mammary cells (MAC-T), antiadhesive properties, and interactions among themselves and with cefoperazone. The oils remained active on milk, were not cytotoxic, and some concentrations stimulated MAC-T cells growth, suggesting healing potential. Subinhibitory concentrations of Coriandrum sativum, Origanum vulgare, Syzygium aromaticum, and Thymus vulgaris reduced biofilm formation by at least 80%. Several oil and cefoperazone combinations displayed additive interaction, with O. vulgare and C. sativum showing the most promising results. We developed formulations for being used as prophylactic postdipping solutions in the field, containing different concentrations (1% or 3%) of the active oils, alone or in combination, with 3% glycerin, 1% Tween 80, and water. The formulations showed strong antimicrobial activity in milk and enhanced antiadhesive properties, specially when two oils were combined in the formula, indicating promising biotechnological and therapeutical applications.<\/jats:p>","DOI":"10.1093\/lambio\/ovad034","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:46:52Z","timestamp":1678841212000},"source":"Crossref","is-referenced-by-count":6,"title":["Formulations of essential oils obtained from plants traditionally used as condiments or traditional medicine active against<i>Staphylococcus aureus<\/i>isolated from dairy cows with mastitis"],"prefix":"10.1093","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5707-3399","authenticated-orcid":false,"given":"Kamylla Rafaella Sena","family":"Albuquerque","sequence":"first","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-2781","authenticated-orcid":false,"given":"Gislaine Aparecida","family":"Purgato","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7873-4977","authenticated-orcid":false,"given":"Mayra Soares","family":"P\u00edccolo","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5486-6617","authenticated-orcid":false,"given":"F\u00e1bio Feres","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8611-4663","authenticated-orcid":false,"given":"Virg\u00ednia Ramos","family":"Pizziolo","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7515-0420","authenticated-orcid":false,"given":"Gaspar Diaz","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departmento de Qu\u00edmica, Universidade Federal de Minas Gerais , Belo Horizonte, MG, 31270-901 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3856-0882","authenticated-orcid":false,"given":"Ciro C\u00e9sar","family":"Rossi","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-4149","authenticated-orcid":false,"given":"Marisa Alves Nogueira","family":"Diaz","sequence":"additional","affiliation":[{"name":"Departmento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa\u2014MG, 36570-000 , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1089\/fpd.2011.1097","article-title":"Evaluation of bacterial resistance to essential oils and antibiotics after exposure to oregano and cinnamon essential oils","volume":"9","author":"Becerril","year":"2012","journal-title":"Foodborne Pathog Dis"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential oils: their antibacterial properties and potential applications in foods\u2014a review","volume":"94","author":"Burt","year":"2004","journal-title":"Int J Food Microbiol"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s12917-022-03197-5","article-title":"Diversity and pathogenesis of Staphylococcus aureus from bovine mastitis: current understanding and future perspectives","volume":"18","author":"Campos","year":"2022","journal-title":"BMC Vet Res"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0168-1605(01)00734-6","article-title":"Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils","volume":"74","author":"Delaquis","year":"2002","journal-title":"Int J Food Microbiol"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3390\/vetsci7040193","article-title":"Use of essential oils in veterinary medicine to combat bacterial and fungal infections","volume":"7","author":"Ebani","year":"2020","journal-title":"Vet Sci"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/s11250-021-02680-9","article-title":"Bovine mastitis prevention and control in the post-antibiotic era","volume":"53","author":"El-Sayed","year":"2021","journal-title":"Trop Anim Health Prod"},{"key":"2023032308593043700_","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.3168\/jds.2015-10538","article-title":"Citrus-derived oil inhibits Staphylococcus aureus growth and alters its interactions with bovine mammary cells","volume":"99","author":"Federman","year":"2016","journal-title":"J Dairy 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