{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T05:23:03Z","timestamp":1770355383701,"version":"3.49.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Small and Medium Enterprises Development Organization of Turkey","award":["51814"],"award-info":[{"award-number":["51814"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enterococci are commensals of the human intestinal tract. Their use as probiotics is supported by their ability to confer several health benefits and eliminate foodborne pathogens but is controversial due to the presence of virulence and antibiotic resistance traits. To use them as probiotics requires thorough research to establish their safety. Here, we sequenced the whole genome of a newly isolated Enterococcus durans MN187066 and used a suite of bioinformatics tools to analyze its beneficial probiotic traits as well as antimicrobial resistance and virulence genes. The whole genome had a length of 2\u2009978\u2009152\u00a0bp, and an average G\u00a0+\u00a0C content of 37.88%. The bopABCD genes involved in biofilm formation were annotated in the genome. However, further analysis showed that these genes are mostly helpful in strengthening their colonization and establishment in the gastrointestinal tract. Also, we identified secondary metabolite gene clusters and the bacteriocins Enterolysin A and Enterocin P. We also identified repUS15 and rep1 replicons and genes that were associated with antimicrobial resistance and virulence. Nevertheless, vancomycin resistance genes were not detected. Our results show that the Ent. durans strain MN187066 can be considered a nontoxigenic strain and produces beneficial metabolites that are critical for their success as probiotics.<\/jats:p>","DOI":"10.1093\/lambio\/ovad035","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:46:52Z","timestamp":1678841212000},"source":"Crossref","is-referenced-by-count":11,"title":["Genome sequence and probiotic potential of newly isolated<i>Enterococcus durans<\/i>strain MN187066"],"prefix":"10.1093","volume":"76","author":[{"given":"Blaise Manga","family":"Enuh","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Science, Eskisehir Osmangazi University , 26040 Eskisehir , Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serap","family":"Gedikli","sequence":"additional","affiliation":[{"name":"Microbiota Biotechnology Industry and Trade Inc Co. , Organized Industrial Zone, 34. Street, 26110 Eski\u015fehir , Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u0131nar","family":"Aytar\u00a0\u00c7elik","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Science, Eskisehir Osmangazi University , 26040 Eskisehir , Turkey"},{"name":"Environmental Protection and Control Program, Eski\u015fehir Osmangazi University , 26110 Eski\u015fehir , Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"\u00c7abuk","sequence":"additional","affiliation":[{"name":"Microbiota Biotechnology Industry and Trade Inc Co. , Organized Industrial Zone, 34. Street, 26110 Eski\u015fehir , Turkey"},{"name":"Department of Biology, Faculty of Science, Eskisehir Osmangazi University , 26040 Eskisehir , Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"2023032410082829100_","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ijfoodmicro.2007.11.067","article-title":"Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples","volume":"123","author":"Abriouel","year":"2008","journal-title":"Int J Food Microbiol"},{"key":"2023032410082829100_","doi-asserted-by":"crossref","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"2023032410082829100_","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1111\/1471-0307.12714","article-title":"Production of set-type yoghurt using Enterococcus faecium and Enterococcus durans strains with probiotic potential as starter adjuncts","volume":"73","author":"Akpinar","year":"2020","journal-title":"Int J Dairy Technol"},{"key":"2023032410082829100_","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1007\/DCR.0b013e3181f4b148","article-title":"E. 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