{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T04:59:33Z","timestamp":1770958773681,"version":"3.50.1"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T00:00:00Z","timestamp":1696723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100022899","name":"SA Water","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100022899","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Effective extraction and detection of viral nucleic acids from sewage are fundamental components of a successful SARS-CoV-2 sewage surveillance programme. As there is no standard method employed in sewage surveillance, understanding the performance of different extraction kits in the recovery of SARS-CoV-2 and the impact that PCR inhibitors have on quantification is essential to minimize data discrepancies caused by sample extraction. Three commercial nucleic acid extraction kits: the RNeasy PowerSoil Total RNA Kit (PS), the RNeasy PowerMicrobiome Kit (PMB), and the MagMAX\u2122 Microbiome Ultra Nucleic Acid Isolation Kit (MM), with minor modifications, were evaluated. Their efficacy in recovering viral ribonucleic acid and removal of PCR inhibitors was assessed using two South Australian wastewater matrices\u2014one from a major metropolitan site and one from a regional centre. Both had SARS-CoV-2 present due to active COVID-19 cases in these communities. Overall, the MM kit had a higher recovery of SARS-CoV-2 from the samples tested, followed by PMB and PS. The PMB kit performance was strongly influenced by the sample matrix when compared to the MM kit. It is recommended to assess the performance of extraction kits using different local wastewater matrices to ensure the accuracy and reliability of monitoring results to avoid false reporting.<\/jats:p>","DOI":"10.1093\/lambio\/ovad136","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T20:47:02Z","timestamp":1701982022000},"source":"Crossref","is-referenced-by-count":2,"title":["Comparison of kits for SARS-CoV-2 extraction in liquid and passive samples"],"prefix":"10.1093","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8605-1439","authenticated-orcid":false,"given":"Melody","family":"Lau","sequence":"first","affiliation":[{"name":"SA Water Utility , 250 Victoria Square, Adelaide SA 5000 , Australia"},{"name":"Future Industries Institute, University of South Australia , Adelaide, SA, 5001 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendon J","family":"King","sequence":"additional","affiliation":[{"name":"SA Water Utility , 250 Victoria Square, Adelaide SA 5000 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Keegan","sequence":"additional","affiliation":[{"name":"SA Water Utility , 250 Victoria Square, Adelaide SA 5000 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Drigo","sequence":"additional","affiliation":[{"name":"Future Industries Institute, University of South Australia , Adelaide, SA, 5001 , Australia"},{"name":"UniSA STEM, University of South Australia , Adelaide, SA 5001 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Donner","sequence":"additional","affiliation":[{"name":"Future Industries Institute, University of South Australia , Adelaide, SA, 5001 , Australia"},{"name":"Cooperative Research Centre for Solving Antimicrobial resistance in Agribusiness, Food, and Environments (CRC SAAFE) , Adelaide, SA 5001 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Monis","sequence":"additional","affiliation":[{"name":"SA Water Utility , 250 Victoria Square, Adelaide SA 5000 , Australia"},{"name":"Future Industries Institute, University of South Australia , Adelaide, SA, 5001 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,10,8]]},"reference":[{"key":"2023121917003608700_bib1","doi-asserted-by":"crossref","first-page":"139960","DOI":"10.1016\/j.scitotenv.2020.139960","article-title":"Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater","volume":"739","author":"Ahmed","year":"2020","journal-title":"Sci Total Environ"},{"key":"2023121917003608700_bib2","doi-asserted-by":"crossref","first-page":"144216","DOI":"10.1016\/j.scitotenv.2020.144216","article-title":"SARS-CoV-2 RNA monitoring in wastewater as a potential early warning system for COVID-19 transmission in the community: a temporal case study","volume":"761","author":"Ahmed","year":"2021","journal-title":"Sci Total Environ"},{"key":"2023121917003608700_bib3","doi-asserted-by":"crossref","first-page":"946","DOI":"10.3390\/pathogens10080946","article-title":"Sewage systems surveillance for SARS-CoV-2: identification of knowledge gaps, emerging threats, and future research needs","volume":"10","author":"Amereh","year":"2021","journal-title":"Pathogens"},{"key":"2023121917003608700_bib16_921_132023","first-page":"76","article-title":"Improved recovery of SARS-CoV-2 from wastewater through application of RNA and DNA stabilising agents","author":"Bell","year":"2023","journal-title":"Letters in Applied Microbiology"},{"key":"2023121917003608700_bib4","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s12560-021-09498-6","article-title":"A State-of-the-art scoping review on SARS-CoV-2 in sewage focusing on the potential of wastewater surveillance for the monitoring of the COVID-19 pandemic","volume":"14","author":"Bonanno\u00a0Ferraro","year":"2022","journal-title":"Food Environ Virol"},{"key":"2023121917003608700_bib5","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-94230-4","volume-title":"DNA and RNA Isolation Techniques for Non-experts","author":"Gautam","year":"2022"},{"key":"2023121917003608700_bib6","volume-title":"ColoSSoS Inter-laboratory Study: SARS-CoV-2 in Wastewater; 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