{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:57:27Z","timestamp":1753876647285,"version":"3.41.2"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The diagnosis of human leptospirosis is mainly based on serological assays. Since the extraction by N-butanol has only been studied as an antigen for the diagnosis of cattle leptospirosis, this study aimed to investigate the feasibility of the N-butanol preparation for the diagnosis of human leptospirosis and compare it with sonicated and thermo-resistant antigens in IgM dot-blot test. Paired serum samples from 147 laboratory-confirmed leptospirosis cases were tested. The control group consisted of 148 serum samples from healthy individuals and nonleptospirosis cases. N-butanol antigens from serovar Copenhageni (ButC3) and serovar Patoc (ButP3) showed reactivity with antileptospiral antibodies from patients with confirmed leptospirosis. In the acute phase, sensitivities of IgM dot-blot assay with ButC3 and ButP3 antigens were 47.6% and 51.0%, respectively. In the convalescent phase, sensitivities were 95.9% (ButC3) and 93.2% (ButP3), and no significant differences were observed among the IgM dot-blot tests with other antigens. The specificity of the IgM dot-blot test with ButC3 antigen was good (92.6%), but with ButP3 (83.1%), it was significantly lower than with the other tests. The IgM dot-blot test described in this study is simple to perform and presents reliable visual results. Antigens prepared by N-butanol proved to be valuable diagnostic markers of leptospirosis.<\/jats:p>","DOI":"10.1093\/lambio\/ovae004","type":"journal-article","created":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T03:02:12Z","timestamp":1705114932000},"source":"Crossref","is-referenced-by-count":1,"title":["Comparative analysis of a novel <i>N<\/i>-butanol-prepared antigen vs thermo-resistant and sonicated antigens for human leptospirosis detection"],"prefix":"10.1093","volume":"77","author":[{"given":"Roberta Morozetti","family":"Blanco","sequence":"first","affiliation":[{"name":"Instituto Adolfo Lutz, Centro de Bacteriologia, Laborat\u00f3rio de Leptospirose , 01246-000 S\u00e3o Paulo, SP , Brazil"},{"name":"Universidade de S\u00e3o Paulo, Faculdade de Medicina, Departamento de Doen\u00e7as Infecciosas e Parasit\u00e1rias, 01246-903 S\u00e3o Paulo, SP , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine dos 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