{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:07:49Z","timestamp":1753877269548,"version":"3.41.2"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000883","name":"University of Bristol","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000883","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Strains of Salmonella Enteritidis (SEnt, n\u00a0=\u00a010) and S. Typhimurium (STm, n\u00a0=\u00a011), representing clones with high impact on human health, and strains of S. 4,12: b:- (S412B n\u00a0=\u00a011) and S. Liverpool (SLiv, n\u00a0=\u00a04), representing clones with minor impact on human health were characterized for 16 growth, stress, and virulence phenotypes to investigate whether systematic differences exist in their performance in these phenotypes and whether there was correlation between performance in different phenotypes. The term serotype was not found to be predictive of a certain type of performance in any phenotype, and surprisingly, on average, strains of SEnt and STm were not significantly better in adhering to and invading cultured intestinal cells than the less pathogenic types. Forest analysis identified desiccation tolerance and the ability to grow at 42\u00b0C with high salt as the characters that separated serovars with low human health impact (S412B\/SLiv) from serovars with high human health impact (SEnt\/STm). The study showed that variation in phenotypes was high even within serovars and correlation between phenotypes was low, i.e. the way that a strain performed phenotypically in one of the tested conditions had a low predictive value for the performance of the strain in other conditions.<\/jats:p>","DOI":"10.1093\/lambio\/ovae015","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T03:41:26Z","timestamp":1708141286000},"source":"Crossref","is-referenced-by-count":0,"title":["Lack of correlation between growth, stress, and virulence phenotypes in strains of <i>Salmonella enterica<\/i> serovar Enteritidis, <i>S<\/i>. Typhimurium DT104, <i>S<\/i>. 4,12, b:- and <i>S<\/i>. Liverpool"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6225-6587","authenticated-orcid":false,"given":"John Elmerdahl","family":"Olsen","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen , 1870 Frederiksberg C. , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorte","family":"Frees","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen , 1870 Frederiksberg C. , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels Christian","family":"Kyvsgaard","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen , 1870 Frederiksberg C. , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Barco","sequence":"additional","affiliation":[{"name":"WOAH, National Reference Laboratory for Salmonella, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 , Legnaro, Padova , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,2,14]]},"reference":[{"key":"2024022117305102600_bib1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/03079450120106615","article-title":"Quantitative comparison of intestinal invasion of zoonotic serotypes of Salmonella enterica in poultry","volume":"31","author":"Aabo","year":"2002","journal-title":"Avian Pathol"},{"key":"2024022117305102600_bib2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/diseases7010019","article-title":"Quantitative risk assessment model of human salmonellosis resulting from consumption of broiler chicken","volume":"7","author":"Akil","year":"2019","journal-title":"Diseases"},{"key":"2024022117305102600_bib3","first-page":"6971","article-title":"The European Union One health 2020 zoonoses report","volume":"19","author":"Anon","year":"2021","journal-title":"EFSA J"},{"key":"2024022117305102600_bib4","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1863-2378.2012.01487.x","article-title":"Application of the random Forest method to analyse epidemiological and phenotypic characteristics of Salmonella 4,[5],12:i:- andSalmonella Typhimurium strains","volume":"59","author":"Barco","year":"2012","journal-title":"Zoonoses Public Health"},{"key":"2024022117305102600_bib5","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.aca.2011.02.010","article-title":"In silico classification of human maximum recommended daily dose based on modified random forest and substructure fingerprint","volume":"692","author":"Cao","year":"2011","journal-title":"Anal Chim Acta"},{"key":"2024022117305102600_bib6","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0378-1135(01)00347-9","article-title":"An epidemiological study of Salmonella enterica serovar 4, 12:b:- in broiler chickens in Denmark","volume":"82","author":"Chadfield","year":"2001","journal-title":"Vet Microbiol"},{"key":"2024022117305102600_bib7","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.fm.2012.03.012","article-title":"Influence of serotype on the growth kinetics and the ability to form biofilms of Salmonella isolates from poultry","volume":"31","author":"Diez-Garcia","year":"2012","journal-title":"Food Microbiol"},{"key":"2024022117305102600_bib8","doi-asserted-by":"crossref","first-page":"7866","DOI":"10.1128\/AEM.01822-12","article-title":"Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium","volume":"78","author":"Gruzdev","year":"2012","journal-title":"Appl Environ Microb"},{"key":"2024022117305102600_bib9","doi-asserted-by":"crossref","first-page":"109462","DOI":"10.1016\/j.ijfoodmicro.2021.109462","article-title":"Relationship between growth ability, virulence, and resistance to food-processing related stresses in non-typhoidal salmonellae","volume":"361","author":"Guillen","year":"2022","journal-title":"Int J Food Microbiol"},{"key":"2024022117305102600_bib10","doi-asserted-by":"crossref","first-page":"e1001923","DOI":"10.1371\/journal.pmed.1001923","article-title":"World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010","volume":"12","author":"Havelaar","year":"2015","journal-title":"PLoS Med"},{"key":"2024022117305102600_bib11","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.3389\/fmicb.2019.01043","article-title":"The effect of previous life cycle phase on the growth kinetics, morphology, and antibiotic resistance of Salmonella Typhimurium DT104 in brain heart infusion and ground chicken extract","volume":"10","author":"Hawkins","year":"2019","journal-title":"Front Microbiol"},{"key":"2024022117305102600_bib12","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.resmic.2014.07.004","article-title":"Supplement 2008\u20132010 (no. 48) to the White-Kauffmann-Le Minor scheme","volume":"165","author":"Issenhuth-Jeanjean","year":"2014","journal-title":"Res Microbiol"},{"key":"2024022117305102600_bib13","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.3389\/fmicb.2017.02000","article-title":"Molecular basis of stationary phase survival and applications","volume":"8","author":"Jaishankar","year":"2017","journal-title":"Front Microbiol"},{"key":"2024022117305102600_bib14","doi-asserted-by":"crossref","first-page":"e36149","DOI":"10.1371\/journal.pone.0036149","article-title":"Polyamines are required for virulence in Salmonella enterica serovar Typhimurium","volume":"7","author":"Jelsbak","year":"2012","journal-title":"PLoS One"},{"key":"2024022117305102600_bib15","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1111\/j.1365-2672.2008.04134.x","article-title":"Model systems allowing quantification of sensitivity to disinfectants and comparison of disinfectant susceptibility of persistent and presumed nonpersistent Listeria monocytogenes","volume":"106","author":"Kastbjerg","year":"2009","journal-title":"J Appl Microbiol"},{"key":"2024022117305102600_bib16","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.chom.2013.11.010","article-title":"An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium","volume":"14","author":"Kroger","year":"2013","journal-title":"Cell Host Microbe"},{"key":"2024022117305102600_bib17","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0168-1605(00)00269-5","article-title":"Hazard identification and exposure assessment for microbial food safety risk assessment","volume":"58","author":"Lammerding","year":"2000","journal-title":"Int J Food Microbiol"},{"key":"2024022117305102600_bib18","doi-asserted-by":"crossref","first-page":"705326","DOI":"10.3389\/fmicb.2021.705326","article-title":"Effects of NaCl concentrations on growth patterns, phenotypes associated with virulence, and energy metabolism in Escherichia coli BW25113","volume":"12","author":"Li","year":"2021","journal-title":"Front Microbiol"},{"key":"2024022117305102600_bib19","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.fm.2010.04.006","article-title":"Effect of the growth environment on the strain variability of Salmonella enterica kinetic behavior","volume":"28","author":"Lianou","year":"2011","journal-title":"Food Microbiol"},{"key":"2024022117305102600_bib20","doi-asserted-by":"crossref","first-page":"e0276040","DOI":"10.1371\/journal.pone.0276040","article-title":"Estimating microbial population data from optical density","volume":"17","author":"Mira","year":"2022","journal-title":"PLoS One"},{"key":"2024022117305102600_bib21","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1111\/jam.15381","article-title":"Differences in biofilm formation of Salmonella serovars on two surfaces under two temperature conditions","volume":"132","author":"Obe","year":"2022","journal-title":"J Appl Microbiol"},{"key":"2024022117305102600_bib22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1099\/00222615-40-1-15","article-title":"Clonal lines of Salmonella enterica serotype Enteritidis documented by IS200-, ribo-, pulsed-field gel electrophoresis and RFLP typing","volume":"40","author":"Olsen","year":"1994","journal-title":"J Med Microbiol"},{"key":"2024022117305102600_bib23","doi-asserted-by":"crossref","first-page":"e02038","DOI":"10.1128\/AEM.02038-18","article-title":"Behavior of foodborne pathogens Listeria monocytogenes and Staphylococcus aureus in mixed-species biofilms exposed to biocides","volume":"84","author":"Oxaran","year":"2018","journal-title":"Appl Environ Microb"},{"key":"2024022117305102600_bib24","doi-asserted-by":"crossref","first-page":"821679","DOI":"10.3389\/fmicb.2022.821679","article-title":"Effect of pH and salinity on the ability of Salmonella serotypes to form biofilm","volume":"13","author":"Petrin","year":"2022","journal-title":"Front Microbiol"},{"key":"2024022117305102600_bib25","doi-asserted-by":"crossref","first-page":"1184387","DOI":"10.3389\/fmicb.2023.1184387","article-title":"Assessing phenotypic virulence of Salmonella enterica across serovars and sources","volume":"14","author":"Petrin","year":"2023","journal-title":"Front Microbiol"},{"key":"2024022117305102600_bib26","first-page":"559","article-title":"Salmonella typhimurium DT104: a virulent and drug-resistant pathogen","volume":"39","author":"Poppe","year":"1998","journal-title":"Can Vet J"},{"key":"2024022117305102600_bib27","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1017\/S0950268800052110","article-title":"Correlation of change in phage type with pulsed field profile and 16S rrn profile in Salmonella enteritidis phage types 4, 7 and 9a","volume":"114","author":"Powell","year":"1995","journal-title":"Epidemiol Infect"},{"key":"2024022117305102600_bib34_934_193224","first-page":"6704","article-title":"Characterization of osmotically induced filaments of Salmonella enterica","volume-title":"Appl Environ Microbiol","author":"Pratt","year":"2012"},{"key":"2024022117305102600_bib28","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1111\/jam.12659","article-title":"Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395","volume":"117","author":"Prol\u00a0Garcia","year":"2014","journal-title":"J Appl Microbiol"},{"key":"2024022117305102600_bib29","doi-asserted-by":"crossref","first-page":"104237","DOI":"10.1016\/j.fm.2023.104237","article-title":"Strain and serovar variants of Salmonella enterica exhibit diverse tolerance to food chain-related stress","volume":"112","author":"Pye","year":"2023","journal-title":"Food Microbiol"},{"key":"2024022117305102600_bib30","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/1471-2180-13-294","article-title":"Non-essential genes form the hubs of genome scale protein function and environmental gene expression networks in Salmonella enterica serovar Typhimurium","volume":"13","author":"Rosenkrantz","year":"2013","journal-title":"BMC Microbiol"},{"key":"2024022117305102600_bib31","doi-asserted-by":"crossref","first-page":"112701","DOI":"10.1016\/j.lwt.2021.112701","article-title":"Machine learning-based predictive modeling to identify genotypic traits associated with Salmonella enterica disease endpoints in isolates from ground chicken","volume":"154","author":"Tanui","year":"2022","journal-title":"LWT"},{"key":"2024022117305102600_bib32","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.ijfoodmicro.2010.09.026","article-title":"Dose-response modeling of Salmonella using outbreak data","volume":"144","author":"Teunis","year":"2010","journal-title":"Int J Food Microbiol"},{"key":"2024022117305102600_bib33","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1128\/AEM.01942-08","article-title":"Changes in barotolerance, thermotolerance, and cellular morphology throughout the life cycle of Listeria monocytogenes","volume":"75","author":"Wen","year":"2009","journal-title":"Appl Environ Microb"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovae015\/56681850\/ovae015.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/77\/2\/ovae015\/56730050\/ovae015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/77\/2\/ovae015\/56730050\/ovae015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T17:31:12Z","timestamp":1708536672000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovae015\/7608515"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,1]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2,1]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovae015","relation":{},"ISSN":["1472-765X"],"issn-type":[{"type":"electronic","value":"1472-765X"}],"subject":[],"published-other":{"date-parts":[[2024,2]]},"published":{"date-parts":[[2024,2,1]]},"article-number":"ovae015"}}