{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:21:53Z","timestamp":1790385713761,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The poultry industry is a very important agricultural and industrial sector in Tunisia and Nigeria, with little information about occurrence of diarrheagenic Escherichia coli in the farmers and chickens. This study aimed to detect the prevalence of diarrheal E. coli in humans and poultry and to investigate plasmid-mediated quinolone resistance (PMQR) genes in both countries. Seventy-four isolates of E. coli were studied; nine different virulence genes were screened by PCR. Serotyping was performed only for pathotypes as well as the determining of antibiotic resistance profiles against 21 antibiotics. PMQR genes were investigated by PCR. EAEC was the most abundant pathotype (37\/74; 50%) in human and chicken isolates, whereas single EHEC and EPEC (1\/74, 1.35%) pathotypes were detected in Tunisia and Nigeria, respectively. About 17 (45.95%) quinolones\/fluoroquinolones-resistant isolates were detected, from which the following PMQR genes were detected: aac(6\u2032)-Ib-cr (8\/17, 47.05%), qepA (6\/17, 35.29%), qnrA\u00a0+\u00a0qnrB (2\/17, 11.76%), and qnrS gene (1\/17, 5.88%). Our findings highlight high occurrence of EAEC pathotype in Tunisia and Nigeria, more frequent than EPEC and EHEC. Additionally, all E. coli pathotypes isolated from different sources (humans, poultry) showed resistance to several antibiotics, which are in use as therapeutic choices in Tunisia and Nigeria.<\/jats:p>","DOI":"10.1093\/lambio\/ovae043","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T03:25:08Z","timestamp":1713842708000},"source":"Crossref","is-referenced-by-count":5,"title":["Pathovars, occurrence, and characterization of plasmid-mediated quinolone resistance in diarrheal <i>Escherichia coli<\/i> isolated from farmers and farmed chickens in Tunisia and Nigeria"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4773-0575","authenticated-orcid":false,"given":"Nazek","family":"AL-GALLAS","sequence":"first","affiliation":[{"name":"Department of Biology, College of Science, University of Hafr Al Batin , P.O. Box 1803, Hafr Al Batin 31991 , Kingdom of Saudi Arabia"},{"name":"Water and Food Control Lab, National Center of Salmonella, Shigella, Vibrio-Enteropathogens\u2014Institut Pasteur de Tunis (IPT) Tunis-Belv\u00e9d\u00e8re , Tunis 1002 , Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed-Elamen","family":"Fadel","sequence":"additional","affiliation":[{"name":"Medical Laboratory Department, Faculty of Engineering and Technology, University of Sebha , Sebha 19631 , Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8691-086X","authenticated-orcid":false,"given":"Khadijah A","family":"Altammar","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, University of Hafr Al Batin , P.O. Box 1803, Hafr Al Batin 31991 , Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Awadi","sequence":"additional","affiliation":[{"name":"Water and Food Control Lab, National Center of Salmonella, Shigella, Vibrio-Enteropathogens\u2014Institut Pasteur de Tunis (IPT) Tunis-Belv\u00e9d\u00e8re , Tunis 1002 , Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ridha Ben","family":"Aissa","sequence":"additional","affiliation":[{"name":"Water and Food Control Lab, National Center of Salmonella, Shigella, Vibrio-Enteropathogens\u2014Institut Pasteur de Tunis (IPT) Tunis-Belv\u00e9d\u00e8re , Tunis 1002 , Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"2024050814253848600_bib1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1128\/AAC.44.2.355-361.2000","article-title":"New gene cassettes for trimethoprim 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