{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:35:30Z","timestamp":1792762530502,"version":"4.5.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["70277"],"award-info":[{"award-number":["70277"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The antibacterial, antibiofilm, and cytotoxicity activity of cell-free supernatants (CFSs) from probiotics, including Lactobacillus plantarum, Bifidobacterium bifidum, and Saccharomyces cerevisiae against multi-drug resistant Escherichia coli evaluated in current research. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the CFSs were determined by analyzing inhibition zone formation using agar disk diffusion for antibacterial activity, microtiter plate for biofilm analysis, and auto-aggregation were done. CFSs substances were analyzed by GC-MS. The MTT assay on HEK293 cells investigated CFS\u2019s influence on cell viability. CFSs were examined for biofilm-related virulence genes, including aggR and fimH using real-time polymerase chain reaction (real-time PCR). All CFSs had bacteriostatic and bactericidal effects. The B. bifidum exhibited the highest antibiofilm activity compared to the others. Bifidobacterium bifidum, L. plantarum, and S. cerevisiae produce 19, 16, and 11\u2009mm inhibition zones against E. coli, respectively. GC-MS indicated that Hydroxyacetone, 3-Hydroxybutyric acid, and Oxime-methoxy-phenyl-dominated CFSs from L. plantarum, B. bifidum, and S. cerevisiae CFSs, respectively. The MTT test demonstrated a cell viability rate of over 90%. Statistically, adding all CFSs lowered the relative expression of both aggR and fimH virulence genes.<\/jats:p>","DOI":"10.1093\/lambio\/ovae084","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T01:56:55Z","timestamp":1725587815000},"source":"Crossref","is-referenced-by-count":23,"title":["Cytotoxicity assessment and antimicrobial effects of cell-free supernatants from probiotic lactic acid bacteria and yeast against multi-drug resistant <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-8820","authenticated-orcid":false,"given":"Mahdi Asghari","family":"Ozma","sequence":"first","affiliation":[{"name":"Student Research Committee, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Ghotaslou","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Asgharzadeh","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences , Tehran 1983969411 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ahangarzadeh","family":"Rezaee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein Samadi","family":"Kafil","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"2024091409391801000_bib1","first-page":"614","article-title":"Effect of Lactobacillus sp. crude bacteriocin (CB) and cell-free supernatant (CFS) against E. coli growth and adherence on vaginal epithelial cell surface","volume":"4","author":"Abbas","year":"2016","journal-title":"Int J Adv Res"},{"key":"2024091409391801000_bib2","doi-asserted-by":"publisher","first-page":"26","DOI":"10.3390\/pediatric16010003","article-title":"Efficacy and safety of Eculizumab in enteroaggregative E. coli associated hemolytic Uremic syndrome","volume":"16","author":"Acharya","year":"2024","journal-title":"Pediatr Rep"},{"key":"2024091409391801000_bib3","doi-asserted-by":"publisher","first-page":"116181","DOI":"10.1016\/j.diagmicrobio.2024.116181","article-title":"Intermethod comparability analyses of gepotidacin antimicrobial susceptibility tests using a large collection of globally collected Escherichia coli and Staphylococcus saprophyticus clinical isolates","volume":"108","author":"Arends","year":"2024","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2024091409391801000_bib4","doi-asserted-by":"publisher","first-page":"1331","DOI":"10.1099\/jmm.0.022475-0","article-title":"Commensal Escherichia coli of healthy humans: a reservoir for antibiotic-resistance determinants","volume":"59","author":"Bailey","year":"2010","journal-title":"J Med Microbiol"},{"key":"2024091409391801000_bib5","doi-asserted-by":"publisher","first-page":"1849","DOI":"10.14202\/vetworld.2023.1849-1865","article-title":"Survivability of freeze-and spray-dried probiotics and their effects on the growth and health performance of broilers","volume":"16","author":"Buahom","year":"2023","journal-title":"Vet World"},{"key":"2024091409391801000_bib6","doi-asserted-by":"publisher","first-page":"602280","DOI":"10.3389\/fvets.2020.602280","article-title":"Comparative studies of inhibitory and antioxidant activities, and organic acids compositions of postbiotics produced by probiotic Lactiplantibacillus plantarum strains isolated from Malaysian foods","volume":"7","author":"Chang","year":"2021","journal-title":"Front Vet Sci"},{"key":"2024091409391801000_bib7","doi-asserted-by":"crossref","first-page":"105197","DOI":"10.1016\/j.jtice.2023.105197","article-title":"Selective screening Lactobacillus spp. against Escherichia coli and Salmonella Typhimurium and optimizing a cane molasses-based medium with Lactobacillus acidophilus as a guiding model","volume":"160","author":"Chin","year":"2023","journal-title":"J Taiwan Inst Chem Eng"},{"key":"2024091409391801000_bib8","doi-asserted-by":"publisher","first-page":"127245","DOI":"10.1016\/j.micres.2022.127245","article-title":"The metabolic, protective, and immune functions of Akkermansia muciniphila","volume":"266","author":"Ghotaslou","year":"2023","journal-title":"Microbiol Res"},{"key":"2024091409391801000_bib9","doi-asserted-by":"publisher","first-page":"e0021321","DOI":"10.1128\/JCM.00213-21","article-title":"Overview of changes to the clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing, M100","volume":"59","author":"Humphries","year":"2021","journal-title":"J Clin Microbiol"},{"key":"2024091409391801000_bib10","doi-asserted-by":"publisher","first-page":"1442","DOI":"10.3390\/microbiolres14030098","article-title":"Postbiotics of the Lactiplantibacillus plantarum EIR\/IF-1 strain show antimicrobial activity against oral microorganisms with pH adaptation capability","volume":"14","author":"Karaca","year":"2023","journal-title":"Microbiol Res"},{"key":"2024091409391801000_bib11","doi-asserted-by":"publisher","first-page":"120","DOI":"10.3389\/fcimb.2018.00120","article-title":"Anti-biofilm properties of the fecal probiotic Lactobacilli against Vibrio spp","volume":"8","author":"Kaur","year":"2018","journal-title":"Front Cell Infect Microbiol"},{"key":"2024091409391801000_bib12","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1002\/9781119246329.ch9","article-title":"Chemical nature of biofilm matrix and its significance","volume-title":"Biofilms in Plant and Soil Health","author":"Khan","year":"2017"},{"key":"2024091409391801000_bib13","doi-asserted-by":"publisher","first-page":"e14562","DOI":"10.1016\/j.heliyon.2023.e14562","article-title":"Microbiota metabolites in the female reproductive system: focused on the short-chain fatty acids","volume":"9","author":"Mirzaei","year":"2023","journal-title":"Heliyon"},{"key":"2024091409391801000_bib14","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.lwt.2019.05.072","article-title":"Characterization and application of postbiotics of Lactobacillus spp. on Listeria monocytogenes in vitro and in food models","volume":"111","author":"Moradi","year":"2019","journal-title":"LWT"},{"key":"2024091409391801000_bib15","doi-asserted-by":"publisher","first-page":"273","DOI":"10.7754\/Clin.Lab.2023.230525","article-title":"The correlation between adhesion genes and biofilm formation among Escherichia coli clinical isolates","volume":"70","author":"Musafer","year":"2024","journal-title":"Clin Lab"},{"key":"2024091409391801000_bib16","first-page":"387","article-title":"Screening and identification of effective thermotolerant lactic acid bacteria producing antimicrobial activity against Escherichia coli and Salmonella sp. resistant to antibiotics","volume":"34","author":"Nitisinprasert","year":"2000","journal-title":"Agric Nat Resour"},{"key":"2024091409391801000_bib17","first-page":"525","article-title":"Antibiotic therapy for pan-drug-resistant infections","volume":"30","author":"Ozma","year":"2022","journal-title":"Infez Med"},{"key":"2024091409391801000_bib18","doi-asserted-by":"publisher","first-page":"105003","DOI":"10.1016\/j.micpath.2021.105003","article-title":"Induction of proteome changes involved in biofilm formation of Enterococcus faecalis in response to gentamicin","volume":"157","author":"Ozma","year":"2021","journal-title":"Microb Pathog"},{"key":"2024091409391801000_bib19","doi-asserted-by":"publisher","first-page":"109387","DOI":"10.1016\/j.foodcont.2022.109387","article-title":"Cell-free supernatants of Bacillus subtilis and Bacillus polyfermenticus inhibit Listeria monocytogenes biofilm formation","volume":"144","author":"Park","year":"2023","journal-title":"Food Control"},{"key":"2024091409391801000_bib20","doi-asserted-by":"publisher","first-page":"3521413","DOI":"10.1155\/2016\/3521413","article-title":"Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: in vitro study","volume":"2016","author":"Paula-Ramos","year":"2016","journal-title":"Sci World J"},{"key":"2024091409391801000_bib21","doi-asserted-by":"publisher","first-page":"9","DOI":"10.3389\/fmicb.2012.00009","article-title":"Extraintestinal pathogenic Escherichia coli: a combination of virulence with antibiotic resistance","volume":"3","author":"Pitout","year":"2012","journal-title":"Front Microbiol"},{"key":"2024091409391801000_bib22","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1007\/s12602-015-9186-6","article-title":"Probiotic potential of Lactobacillus strains isolated from sorghum-based traditional fermented food","volume":"7","author":"Rao","year":"2015","journal-title":"Probiotics Antimicrob Proteins"},{"key":"2024091409391801000_bib23","doi-asserted-by":"publisher","first-page":"2172","DOI":"10.1080\/10408398.2020.1851169","article-title":"Effectiveness and mechanisms of essential oils for biofilm control on food-contact surfaces: an updated review","volume":"62","author":"Rossi","year":"2022","journal-title":"Crit Rev Food Sci Nutr"},{"key":"2024091409391801000_bib24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.34172\/PS.2020.39","article-title":"Prophylactic role of Lactobacillus paracasei exopolysaccharides on colon cancer cells through apoptosis not ferroptosis","volume":"27","author":"Saadat","year":"2020","journal-title":"Pharm Sci"},{"key":"2024091409391801000_bib25","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1016\/j.idairyj.2005.12.007","article-title":"Stability and functionality of freeze-dried probiotic bifidobacterium cells during storage in juice and milk","volume":"16","author":"Saarela","year":"2006","journal-title":"Int Dairy J"},{"key":"2024091409391801000_bib26","doi-asserted-by":"publisher","first-page":"8375","DOI":"10.1080\/10408398.2022.2056727","article-title":"Postbiotics as the new frontier in food and pharmaceutical research","volume":"63","author":"Sabahi","year":"2023","journal-title":"Crit Rev Food Sci Nutr"},{"key":"2024091409391801000_bib27","doi-asserted-by":"publisher","first-page":"18","DOI":"10.61838\/kman.jpsad.2.1.4","article-title":"Study of resistance to cephalosporin compounds in Salmonella spp. and Salmonella Typhimurium isolated from turkeys","volume":"2","author":"Samadi","year":"2024","journal-title":"J Poult Sci Avian Dis"},{"key":"2024091409391801000_bib28","doi-asserted-by":"publisher","first-page":"5416","DOI":"10.3168\/jds.2024-24331","article-title":"High-resolution melting real-time PCR assays for subtyping of five diarrheagenic Escherichia coli by a single well in milk","volume":"107","author":"Shan","year":"2024","journal-title":"J Dairy Sci"},{"key":"2024091409391801000_bib29","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1007\/s00203-020-01823-0","article-title":"Antimicrobial and antibiofilm activities of Clostridium butyricum supernatant against Acinetobacter baumannii","volume":"202","author":"Shin","year":"2020","journal-title":"Arch Microbiol"},{"key":"2024091409391801000_bib30","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.jmii.2022.06.006","article-title":"Green synthesis of nano-liposomes containing Bunium persicum and Trachyspermum ammi essential oils against Trichomonas vaginalis","volume":"56","author":"Siyadatpanah","year":"2023","journal-title":"J Microbiol Immunol Infect"},{"key":"2024091409391801000_bib31","doi-asserted-by":"publisher","first-page":"754","DOI":"10.3390\/antibiotics12040754","article-title":"Use of probiotics to control biofilm formation in food industries","volume":"12","author":"Tom\u00e9","year":"2023","journal-title":"Antibiotics"},{"key":"2024091409391801000_bib32","doi-asserted-by":"publisher","first-page":"167576","DOI":"10.1016\/j.scitotenv.2023.167576","article-title":"Enhancing organic matter productivity in microalgal-bacterial biofilm using novel bio-coating","volume":"906","author":"Tong","year":"2024","journal-title":"Sci Total Environ"},{"key":"2024091409391801000_bib33","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/s13679-020-00379-w","article-title":"Probiotics, prebiotics, synbiotics, postbiotics, and obesity: current evidence, controversies, and perspectives","volume":"9","author":"Vallianou","year":"2020","journal-title":"Curr Obes Rep"},{"key":"2024091409391801000_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41594-023-01154-w","article-title":"General transcription factor from Escherichia coli with a distinct mechanism of action","volume":"31","author":"Vasilyev","year":"2024","journal-title":"Nat Struct Mol Biol"},{"key":"2024091409391801000_bib35","doi-asserted-by":"publisher","first-page":"99976","DOI":"10.1039\/C5RA20224H","article-title":"Additive-free 1, 4-butanediol mediated synthesis: a suitable route to obtain nanostructured, mesoporous spherical zinc oxide materials with multifunctional properties","volume":"5","author":"Visinescu","year":"2015","journal-title":"RSC Adv"},{"key":"2024091409391801000_bib36","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. CLSI Document M100-S27","author":"Weinstein","year":"2017"},{"key":"2024091409391801000_bib37","doi-asserted-by":"publisher","first-page":"110","DOI":"10.2174\/1389557523666230608163823","article-title":"Probiotics and their beneficial health effects","volume":"24","author":"Zaib","year":"2024","journal-title":"MRMC"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovae084\/59029195\/ovae084.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/77\/9\/ovae084\/59118249\/ovae084.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/77\/9\/ovae084\/59118249\/ovae084.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T10:16:44Z","timestamp":1726309004000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovae084\/7750035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9,2]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovae084","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,9]]},"published":{"date-parts":[[2024,9]]},"article-number":"ovae084"}}