{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:46:14Z","timestamp":1780465574068,"version":"3.54.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Legionnaires\u2019 disease (LD) is a severe and potentially fatal form of bacterial pneumonia caused by Legionella spp. We evaluated the use of UV-light for detecting Legionella non-pneumophila in water samples according to the NEN-EN-ISO 11731:2017 methodology (reference method) in a collaborative effort involving 10 laboratories. First, a test panel was constructed of 298 strains: 157 Legionella strains and 141 non-Legionella strains were cultured on buffered charcoal yeast extract (BCYE)-medium and confirmed according to ISO 11731: 2017 (cultured on BCYE agar plates with and without l-cysteine), and by matrix-assisted laser desorption-ionization time of flight or next generation sequencing. All strains were additionally exposed to an UV-light to assess if they showed a bright blue fluorescence effect (UV-positive) or not (UV-negative). Second, in an interlaboratory study, 10 laboratories analyzed a blinded set of 16 Legionella strains and 8 non-Legionella strains using both methods. The test panel analyses showed 100% accordance between the UV-light and reference method. In addition, the interlaboratory study results showed full agreement between both methods. Our results support the implementation of UV-light detection to confirm Legionella presumptive colonies during analyses of water samples according to the NEN-EN-ISO 11731:2017 methodology. Implementation of UV-light confirmation could reduce workload, time-to-result and costs for the analyses of water samples for the presence of Legionella.<\/jats:p>","DOI":"10.1093\/lambio\/ovae094","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T03:51:11Z","timestamp":1728013871000},"source":"Crossref","is-referenced-by-count":2,"title":["UV-light fluorescence as a confirmation method for presumptive <i>Legionella<\/i> colonies isolated from water samples"],"prefix":"10.1093","volume":"77","author":[{"given":"Paul","family":"Badoux","sequence":"first","affiliation":[{"name":"Regional Public Health Laboratory Kennemerland , Boerhaavelaan 26, 2035 RC, Haarlem ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrie","family":"Atsma","sequence":"additional","affiliation":[{"name":"Vitens Water Expertise Centre , Snekertrekweg 61, 8912 AA, Leeuwarden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"van\u00a0Harmelen-Vrins","sequence":"additional","affiliation":[{"name":"RPS Analyse BV , Minervum 7002, 4817 ZL, Breda ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sjoerd","family":"Euser","sequence":"additional","affiliation":[{"name":"Regional Public Health Laboratory Kennemerland , Boerhaavelaan 26, 2035 RC, Haarlem ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,10,3]]},"reference":[{"key":"2024101612213852200_bib1","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1016\/j.bbrc.2004.08.180","article-title":"Legioliulin, a new isocoumarin compound responsible for blue-white autofluorescence in Legionella (Fluoribacter) dumoffii under long-wavelength UV light","volume":"323","author":"Amemura-Maekawa","year":"2004","journal-title":"Biochem Biophys Res Commun"},{"key":"2024101612213852200_bib2","article-title":"influenza and other respiratory infections in the Netherlands: winter 2020\/2021, RIVM","year":"2022"},{"key":"2024101612213852200_bib3","volume-title":"Water supply Decree. 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