{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T19:58:24Z","timestamp":1784663904380,"version":"3.55.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Department of Science and Technology (DST)-Science and Engineering Research Board (SERB), National Post-Doctoral Fellowship","award":["PDF\/2019\/002914"],"award-info":[{"award-number":["PDF\/2019\/002914"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Precise and timely diagnosis is essential to prevent severe outcomes of leptospirosis in humans and animals. Existing diagnostic methods face challenges and limitations, underscoring the need for novel, field-applicable screening, and diagnostic tests\/assays. This study evaluates the diagnostic utility of a recombinant ErpY-like lipoprotein (rErpY-LIC11966) in a latex agglutination test (LAT) for diagnosis of animal leptospirosis. The ErpY gene sequence from Leptospira interrogans serovar Pomona, excluding the signal peptide, was amplified, cloned into the pETite vector, and expressed in Escherichia coli. The expressed rErpY (\u223c16\u00a0kDa) was characterized by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Western blot using Leptospira-specific standard sera. To assess the diagnostic potential of rErpY, Ni-NTA affinity-purified protein was used to sensitize latex-coated beads (0.8\u00a0\u00b5m colour beads), which were then employed in the LAT for standardization and optimization with standard positive and negative sera. For evaluation, the rErpY-LAT was tested on serum samples from 177 suspected animal cases and compared to the microscopic agglutination test. It showed a relative diagnostic sensitivity of 90.6%, a specificity of 89.1%, and an overall accuracy of 90%. This study proposes rErpY-LAT as a field testing\/screening diagnostic tool for preliminary serodiagnosis of leptospirosis, highlighting the potential of recombinant protein-based assays to address current diagnostic challenges.<\/jats:p>","DOI":"10.1093\/lambio\/ovae100","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T06:05:31Z","timestamp":1729663531000},"source":"Crossref","is-referenced-by-count":2,"title":["Detection of anti-leptospiral antibodies using recombinant ErpY-like lipoprotein based latex agglutination test for serodiagnosis of animal leptospirosis"],"prefix":"10.1093","volume":"77","author":[{"given":"Kirubakaran Vinod","family":"Kumar","sequence":"first","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prajakta Prashant","family":"Bokade","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Pal","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oviya","family":"Deenadayalan","sequence":"additional","affiliation":[{"name":"Lambert High School , 805 Nichols Road, Suwanee, GA 30024 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashikumar","family":"SowjanyaKumari","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatappa","family":"Bharath","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibek Ranjan","family":"Shome","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka , Bengaluru 560064 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinayagamurthy","family":"Balamurugan","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research\u2014National 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