{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T23:03:09Z","timestamp":1775084589256,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["# TB.BT\/PR20863\/MED\/30\/1889\/2017"],"award-info":[{"award-number":["# TB.BT\/PR20863\/MED\/30\/1889\/2017"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Despite the advent of Xpert MTB\/RIF, pleural tuberculosis (TB) diagnosis in pleural fluid is still difficult. Hence, we assessed the diagnostic efficacy of its advanced version, Xpert MTB\/RIF Ultra, for pleural TB diagnosis using pleural fluid as a sample. Tuberculosis pleuritis (TBP) suspects (n\u00a0=\u00a0261) were enrolled in the study, of which 29 were excluded. The remaining patients (n\u00a0=\u00a0232) were categorized into definite TBP (n\u00a0=\u00a031), probable TBP (n\u00a0=\u00a028), and non-TB controls (n\u00a0=\u00a0173) based on the composite reference standard consisting of smear, culture, histopathology, and Xpert MTB\/RIF as well as follow-up\/clinical response to anti-TB therapy. Among the TBP suspects, 59 were diagnosed as TBP patients. The sensitivity of Xpert MTB\/RIF Ultra (52.5%) using pleural fluid for TBP diagnosis was higher than sensitivity obtained with smear (22.4%), culture (17.6%), and Xpert MTB\/RIF (25%) alone, carried out using either pleural fluid or pleural biopsy or both the samples. In cases of probable TBP, where none of the laboratory tests were positive, Xpert MTB\/RIF Ultra use led to an increased diagnostic percentage of definite TBP from 52.5% to 69.4%. Overall, Xpert MTB\/RIF Ultra showed promising results for a definitive diagnosis of TBP in pleural fluid samples.<\/jats:p>","DOI":"10.1093\/lambio\/ovae102","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T09:46:49Z","timestamp":1730368009000},"source":"Crossref","is-referenced-by-count":1,"title":["Xpert MTB\/RIF Ultra as a diagnostic tool for pleural tuberculosis: a study at Indian tertiary care centre"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3500-7734","authenticated-orcid":false,"given":"Sumedha","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabhdeep","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashutosh N","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khushpreet","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Sethi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indu","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Post Graduate Institute of Medical Education and Research , Chandigarh 160012 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"2024112816515820200_bib1","doi-asserted-by":"publisher","first-page":"e00258","DOI":"10.1128\/JCM.00258-18","article-title":"Diagnostic performance of pleural fluid adenosine deaminase for tuberculous pleural effusion in a low-incidence setting","volume":"56","author":"Blakiston","year":"2018","journal-title":"J Clin Microbiol"},{"key":"2024112816515820200_bib2","doi-asserted-by":"publisher","first-page":"2217","DOI":"10.1038\/s41598-024-52130-3","article-title":"Usefulness of Xpert MTB\/RIF Ultra for rapid diagnosis of extrapulmonary tuberculosis in Tunisia","volume":"14","author":"Bouzouita","year":"2024","journal-title":"Sci Rep"},{"key":"2024112816515820200_bib3","doi-asserted-by":"publisher","first-page":"ci ac332","DOI":"10.1093\/cid\/ciac332","article-title":"Oral swabs tested with Xpert MTB\/RIF Ultra for diagnosis of pulmonary tuberculosis in children: a diagnostic accuracy study","volume":"75","author":"Cox","year":"2022","journal-title":"Clin Infect Dis"},{"key":"2024112816515820200_bib4","doi-asserted-by":"publisher","first-page":"79","DOI":"10.4021\/jocmr2010.03.280w","article-title":"Role of adenosine deaminase estimation in differentiation of tuberculous and non-tuberculous exudative pleural effusions","volume":"2","author":"Gupta","year":"2010","journal-title":"J Clin Med Res"},{"key":"2024112816515820200_bib5","doi-asserted-by":"publisher","first-page":"13959","DOI":"10.1038\/s41598-020-70613-x","article-title":"Xpert MTB\/RIF Ultra for rapid diagnosis of extrapulmonary tuberculosis in a high income low tuberculosis prevalence setting","volume":"10","author":"Hoel","year":"2020","journal-title":"Sci Rep"},{"key":"2024112816515820200_bib6","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1186\/s12879-018-3196-4","article-title":"Is Xpert MTB\/RIF appropriate for diagnosing tuberculous pleurisy with pleural fluid samples? 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