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In this context, this study explores the antimicrobial and antibiofilm potential of the aquatic plant Hydrocleys nymphoides as a promising alternative. Extracts from the plant\u2019s leaves and roots were obtained using solvents of increasing polarity and tested against five key pathogenic Staphylococcus species: S. aureus, S. epidermidis, S. haemolyticus, S. pseudintermedius, and S. coagulans. The hexane extract of H. nymphoides leaves showed the most notable activity, with inhibition zones of 9\u201317\u00a0mm and minimum inhibitory concentrations (MICs) as low as 0.8\u00a0mg\/ml for certain strains. Subinhibitory concentrations of the extract significantly reduced biofilm formation in most strains, with reductions up to 46.9%. Gas chromatography\u2013mass spectrometry revealed bioactive compounds such as linoleic acid, n-hexadecanoic acid, 9-octadecenal, eicosane, and tetratriacontane, known for their antimicrobial and antibiofilm properties. Although cytotoxicity was observed at concentrations near the MIC, lower concentrations were non-toxic, indicating potential for controlled therapeutic applications. These findings underscore the biotechnological value of H. nymphoides as a sustainable source of antimicrobial agents against multidrug-resistant Staphylococcus. This work emphasizes the critical role of phytotherapy in combating the escalating antimicrobial resistance crisis.<\/jats:p>","DOI":"10.1093\/lambio\/ovaf046","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T02:30:52Z","timestamp":1743647452000},"source":"Crossref","is-referenced-by-count":2,"title":["<i>Hydrocleys nymphoides<\/i> (water poppy): a cosmopolitan aquatic plant with a phytochemical-rich extract exhibiting antimicrobial and antibiofilm activity against multidrug-resistant <i>Staphylococcus<\/i>"],"prefix":"10.1093","volume":"78","author":[{"given":"Marcos","family":"Toneto\u00a0Francisco","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Molecular, Universidade Federal de Vi\u00e7osa , Vi\u00e7osa, 36570-000, MG ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro 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