{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:08:26Z","timestamp":1753877306371,"version":"3.41.2"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Tianjin Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["17JCYBJC24300"],"award-info":[{"award-number":["17JCYBJC24300"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation of Tianjin Education Commission","award":["2024ZXZD015"],"award-info":[{"award-number":["2024ZXZD015"]}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2022MH318"],"award-info":[{"award-number":["ZR2022MH318"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Yersinia enterocolitica is one of the most important foodborne pathogens with significant impact on public health. It can be divided into six biotypes and approximately 60 O-serotypes, with O:3, O:9, O:8, and O:5,27 being predominantly associated with human yersiniosis. We present a two-group quadruplex real-time quantitative PCR (RT\u2012qPCR) for the patho-serotyping of Y. enterocolitica. The design of primers and probes within group 1 was based on sero-specific genes within the O antigen gene cluster, and those within group 2 were selected from the virulence markers and the restriction modification system to distinguish O:5,27 from O:5. The specificity was tested using reference strains, and was confirmed by a comparison with those obtained by a previous multiplex PCR. The limit of detection is 0.1\u00a0ng, or 104 copies \u03bcl\u22121 of genomic DNA, and the standard curves exhibited high linearity and correlation coefficients, demonstrating our assay\u2019s robustness. Among the 81 isolates used to evaluate the reproducibility, the results for 76 were consistent between the two approaches, indicating that the sensitivity of our RT\u2012qPCR is 100%, and the positive predictive value is 94%. Our assay can serve as a tool for identifying sources of Y. enterocolitica contamination and for epidemiological monitoring of this bacterium.<\/jats:p>","DOI":"10.1093\/lambio\/ovaf079","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T16:27:54Z","timestamp":1748622474000},"source":"Crossref","is-referenced-by-count":0,"title":["A two-group quadruplex real-time quantitative PCR assay for molecular patho-serotyping of <i>Yersinia enterocolitica<\/i>"],"prefix":"10.1093","volume":"78","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , 23 Hongda Street, TEDA, Tianjin ,","place":["P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingliang","family":"Qin","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , 23 Hongda Street, TEDA, Tianjin ,","place":["P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Hu","sequence":"additional","affiliation":[{"name":"Shandong Center for Disease Control and Prevention , 16992 City Ten Road, Jinan 250014, Shandong ,","place":["P.R. China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijia","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Union Medical Center, the First Affiliated Hospital of Nankai University Department of Coloproctology, , 190 Jieyuan Road, Tianjin 300121 ,","place":["P.R. 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