{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:59:47Z","timestamp":1769277587778,"version":"3.49.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"name":"School of Pharmacy and Biochemistry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Burkholderia cepacia complex (Bcc) bacteria are opportunistic pathogens that particularly affect people with cystic fibrosis and other immunosuppressed patients. These bacteria are also frequent contaminants of industrial products such as medicines and cosmetics, posing a risk to patients and raising public health concerns. Due to numerous outbreaks caused by contaminated products reported worldwide, the absence of Bcc in drugs has become mandatory under various codifications. For example, the US Pharmacopoeia recently incorporated testing for Bcc detection in non-sterile products (USP\u00a0&amp;lt;\u00a060 &amp;gt;). Detection of Bcc usually involves the use of the Burkholderia cepacia selective agar (BCSA), a selective but non-differential culture medium. In this study, we compared a recently developed chromogenic agar, CHROMagar\u2122 B.cepacia, with BCSA for the recovery of Bcc and inhibition of non-Bcc species. We also evaluated both media for the detection of Bcc in artificially contaminated pharmaceutical and cosmetic samples. Both media exhibited similar inhibitory properties. Notably, all Bcc isolates in this study developed blue\u2013green colonies on CHROMagar\u2122 B.cepacia, enabling rapid and presumptive identification. These results suggest that CHROMagar\u2122 B.cepacia could be a valuable tool for the rapid detection of Bcc in industrial samples.<\/jats:p>","DOI":"10.1093\/lambio\/ovaf141","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T12:48:12Z","timestamp":1766234892000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a chromogenic agar medium for the detection of\n                    <i>Burkholderia cepacia<\/i>\n                    complex from industrial samples"],"prefix":"10.1093","volume":"79","author":[{"given":"Tom\u00e1s","family":"Herrera\u00a0Durigneux","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires , Ciudad Aut\u00f3noma de Buenos Aires, 1428 ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juli\u00e1n","family":"Mantovano","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Salud P\u00fablica e Higiene Ambiental. Facultad de Farmacia y Bioqu\u00edmica. Universidad de Buenos Aires , Ciudad Aut\u00f3noma de Buenos Aires, 1113 ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Degrossi","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Salud P\u00fablica e Higiene Ambiental. Facultad de Farmacia y Bioqu\u00edmica. Universidad de Buenos Aires , Ciudad Aut\u00f3noma de Buenos Aires, 1113 ,","place":["Argentina"]},{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM). Facultad de Farmacia y Bioqu\u00edmica. Universidad de Buenos Aires , Ciudad Aut\u00f3noma de Buenos Aires 1113 ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"2026012304120309000_bib1","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1007\/s10295-020-02287-3","article-title":"A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics","volume":"47","author":"Ahn","year":"2020","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2026012304120309000_bib2","doi-asserted-by":"publisher","first-page":"1495","DOI":"10.4014\/jmb.1906.06024","article-title":"Oligotrophic media compared with a tryptic soy agar or broth for the recovery of Burkholderia cepacia complex from different storage temperatures and culture conditions","volume":"10","author":"Ahn","year":"2019","journal-title":"J Microbiol Biotechnol"},{"key":"2026012304120309000_bib3","doi-asserted-by":"publisher","first-page":"458","DOI":"10.3201\/eid1303.060403","article-title":"Environmental Burkholderia cepacia complex isolates in human infections","volume":"13","author":"Baldwin","year":"2007","journal-title":"Emerg Infect Dis"},{"key":"2026012304120309000_bib4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jcf.2013.09.004","article-title":"Culture-based diagnostic microbiology in cystic fibrosis: can we simplify the complexity?","volume":"13","author":"Burns","year":"2014","journal-title":"J Cyst Fibros"},{"key":"2026012304120309000_bib5","doi-asserted-by":"publisher","first-page":"636502","DOI":"10.1155\/2009\/636502","article-title":"Shortened time to identify Staphylococcus species from blood cultures and methicillin resistance testing using CHROMAgar","volume":"2009","author":"Chihara","year":"2009","journal-title":"Int J Microbiol"},{"key":"2026012304120309000_bib6","doi-asserted-by":"publisher","first-page":"106082","DOI":"10.1016\/j.mimet.2020.106082","article-title":"Evaluation of a new chromogenic agar for the detection of environmental Enterococcus","volume":"178","author":"Cho","year":"2020","journal-title":"J Microbiol Methods"},{"key":"2026012304120309000_bib7","doi-asserted-by":"publisher","first-page":"e0012721","DOI":"10.1128\/Spectrum.00127-21","article-title":"Presence of the Hmq system and production of 4-hydroxy-3-Methyl-2-alkylquinolines are heterogeneously distributed between Burkholderia cepacia complex species and more prevalent among environmental than clinical isolates","volume":"9","author":"Coulon","year":"2021","journal-title":"Microbiol Spectr"},{"key":"2026012304120309000_bib8","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.3390\/pathogens10091071","article-title":"Loop-Mediated Isothermal Amplification (LAMP) assay for detecting Burkholderia cepacia complex in non-sterile pharmaceutical products","volume":"10","author":"Daddy\u00a0Gaoh","year":"2021","journal-title":"Pathogens"},{"key":"2026012304120309000_bib10","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s10123-020-00151-z","article-title":"Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina","volume":"24","author":"De\u00a0Volder","year":"2021","journal-title":"Int Microbiol"},{"key":"2026012304120309000_bib31_662_024826","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s12941-019-0306-0","article-title":"Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update","volume":"18","author":"Devanga\u00a0Ragupathi","year":"2019","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"2026012304120309000_bib11","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1016\/j.ajic.2017.11","article-title":"A multistate investigation of health care-associated Burkholderia cepacia complex infections related to liquid docusate sodium contamination, January-October 2016","volume":"46","author":"Glowicz","year":"2018","journal-title":"Am J Infect Control"},{"key":"2026012304120309000_bib12","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1128\/jcm.35.3.614-619.1997","article-title":"Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium","volume":"35","author":"Henry","year":"1997","journal-title":"J Clin Microbiol"},{"key":"2026012304120309000_bib13","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1099\/jmm.0.001136","article-title":"Evaluation of chromogenic selective agar (CHROMagar STEC) for the direct detection of Shiga toxin-producing Escherichia coli from faecal specimens","volume":"69","author":"Jenkins","year":"2020","journal-title":"J Med Microbiol"},{"key":"2026012304120309000_bib14","doi-asserted-by":"publisher","first-page":"73","DOI":"10.5731\/pdajpst.2017.007971","article-title":"Real-time PCR detection of Burkholderia cepacia in pharmaceutical products contaminated with low levels of bacterial contamination","volume":"72","author":"Jimenez","year":"2018","journal-title":"PDA J Pharm Sci Technol"},{"key":"2026012304120309000_bib15","first-page":"383","article-title":"Microbial diversity in pharmaceutical product recalls and environments","volume":"61","author":"Jimenez","year":"2007","journal-title":"PDA J Pharm Sci Technol"},{"key":"2026012304120309000_bib16","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1111\/j.1365-2672.2007.03706.x","article-title":"Burkholderia cepacia Complex bacteria: opportunistic pathogens with important natural biology","volume":"104","author":"Mahenthiralingam","year":"2008","journal-title":"J App Microbiol"},{"key":"2026012304120309000_bib17","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1128\/JCM.38.9.3165-3173.2000","article-title":"DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III","volume":"38","author":"Mahenthiralingam","year":"2000","journal-title":"J Clin Microbiol"},{"key":"2026012304120309000_bib18","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/j.jhin.2010.10.004","article-title":"Hospital-wide outbreak of Burkholderia contaminans caused by prefabricated moist washcloths","volume":"77","author":"Martin","year":"2011","journal-title":"J Hosp Infect"},{"key":"2026012304120309000_bib19","doi-asserted-by":"publisher","first-page":"1349","DOI":"10.1007\/s10096-024-04845-4","article-title":"Evaluation of CHROMagar\u2122 B.cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis","volume":"43","author":"Maruri-Aransolo","year":"2024","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2026012304120309000_bib20","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1111\/j.1365-2672.1980.tb04719.x","article-title":"Quality control of solid culture media: a comparison of the classic and the so-called ecometric technique","volume":"49","author":"Mossel","year":"1980","journal-title":"J Appl Bacteriol"},{"key":"2026012304120309000_bib21","article-title":"Disposition 6967\/2022","volume-title":"Official Bulletin of the Argentine Republic","author":"National Administration of Drugs, Food and Medical Devices (ANMAT)","year":"2022"},{"key":"2026012304120309000_bib22","doi-asserted-by":"publisher","first-page":"2593","DOI":"10.1007\/s10096-021-04328-w","article-title":"Evaluation of CHROMagar\u2122-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens","volume":"40","author":"P\u00e9rez-Viso","year":"2021","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2026012304120309000_bib23","doi-asserted-by":"publisher","first-page":"1610","DOI":"10.1093\/cid\/ciac200","article-title":"Cluster of Burkholderia cepacia complex infections associated with extracorporeal membrane oxygenation water heater devices","volume":"29","author":"Rhee","year":"2022","journal-title":"Clin Infect Dis"},{"key":"2026012304120309000_bib24","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/B978-0-08-100022-9.00010-4","article-title":"Microbiological culture media","volume-title":"Pharmaceutical Microbiology: Essentials for Quality Assurance and Quality Control","author":"Sandle","year":"2016"},{"key":"2026012304120309000_bib25","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.3201\/eid2506.172119","article-title":"Phenotypic and genomic analyses of Burkholderia stabilis clinical contamination, Switzerland","volume":"25","author":"Seth-Smith","year":"2019","journal-title":"Emerg Infect Dis"},{"key":"2026012304120309000_bib26","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.jhin.2017.09.012","article-title":"Outbreak of Burkholderia cepacia pseudobacteraemia caused by intrinsically contaminated commercial 0.5% chlorhexidine solution in neonatal intensive care units","volume":"98","author":"Song","year":"2018","journal-title":"J Hosp Infect"},{"key":"2026012304120309000_bib27","doi-asserted-by":"publisher","first-page":"e00139","DOI":"10.1128\/CMR.00139-19","article-title":"Burkholderia cepacia complex bacteria: a feared contamination risk in water-based pharmaceutical products","volume":"33","author":"Tavares","year":"2020","journal-title":"Clin Microbiol Rev"},{"key":"2026012304120309000_bib28","doi-asserted-by":"publisher","DOI":"10.31003\/USPNF_M12455_02_01","article-title":"General Chapter (60) Microbiological Examination of Nonsterile Products-tests for Burkholderia cepacia complex","volume-title":"USP-NF","author":"United States Pharmacopeia","year":"2019"},{"key":"2026012304120309000_bib29","doi-asserted-by":"publisher","DOI":"10.31003\/USPNF_M98800_02_01","article-title":"General Chapter (61) Microbiological examination of nonsterile products: microbial enumeration tests","volume-title":"USP-NF","author":"United States Pharmacopeia","year":"2025"},{"key":"2026012304120309000_bib30","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.mimet.2008.06.016","article-title":"Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry of intact cells allows rapid identification of Burkholderia cepacia complex","volume":"75","author":"Vanlaere","year":"2008","journal-title":"J Microbiol Methods"},{"key":"2026012304120309000_bib31","doi-asserted-by":"publisher","first-page":"ovac051","DOI":"10.1093\/lambio\/ovac051","article-title":"Evaluation of CHROMagar Acinetobacter and MacConkey media for the recovery of Acinetobacter baumannii from soil samples","volume":"76","author":"Yusuf","year":"2023","journal-title":"Lett Appl Microbiol"}],"container-title":["Letters in Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/lambio\/advance-article-pdf\/doi\/10.1093\/lambio\/ovaf141\/66098170\/ovaf141.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/1\/ovaf141\/66098170\/ovaf141.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/lambio\/article-pdf\/79\/1\/ovaf141\/66098170\/ovaf141.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:12:07Z","timestamp":1769159527000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/lambio\/article\/doi\/10.1093\/lambio\/ovaf141\/8400328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,14]]}},"URL":"https:\/\/doi.org\/10.1093\/lambio\/ovaf141","relation":{},"ISSN":["1472-765X"],"issn-type":[{"value":"1472-765X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,1]]},"published":{"date-parts":[[2025,12,22]]},"article-number":"ovaf141"}}