{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:23:55Z","timestamp":1773865435176,"version":"3.50.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Opella"},{"name":"Sanofi Consumer Healthcare"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alkalihalobacillus clausii is effective in alleviating PPI-induced dysbiosis; however, its effects on gut barrier function and inflammation remain unclear. This study explored A. clausii (O\/C, N\/R, SIN, and T) effects on gut barrier function during PPI-induced dysbiosis using Triple-M-SHIME\u00ae model. Reactors were supplemented with PPI alone (PPI control); simultaneous PPI\u00a0+\u00a0A. clausii (preventive); and PPI for 2 weeks followed by A. clausii (curative). Caco-2\/THP1-Blue\u2122 co-cultures were exposed to colonic SHIME\u00ae suspensions for 24\u00a0h for TEER measurement, followed by lipopolysaccharide stimulation for measurement of cytokine secretion and NF-\u03baB. Preventive treatment did not improve inflammation-induced barrier disruption, but was slightly improved with curative treatment, particularly in the distal colon. Colonic ferments following A. clausii supplementation increased IL-10 secretion and pro-inflammatory signals in the ileum. In the proximal and distal colon, PPI treatment reduced pro-inflammatory markers during and after treatment, while increasing anti-inflammatory cytokines following prolonged incubation. Preventive treatment with A. clausii, reduced pro-inflammatory markers and IL-10, while increasing IL-6 secretion. Curative A. clausii treatment lowered pro-inflammatory markers. Enhanced colonic fermentation by A. clausii thus showed potential in improving gut barrier function and modulating inflammation with complex colon region-specific responses, particularly with curative treatment.<\/jats:p>","DOI":"10.1093\/lambio\/ovag025","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:37:58Z","timestamp":1771591078000},"source":"Crossref","is-referenced-by-count":0,"title":["Impact of a blend of four\n                    <i>Alkalihalobacillus clausii<\/i>\n                    strains (O\/C, N\/R, SIN, and T) on gut barrier integrity and immune modulation with PPIs: an\n                    <i>in vitro<\/i>\n                    SHIME study"],"prefix":"10.1093","volume":"79","author":[{"given":"Lynn","family":"Verstrepen","sequence":"first","affiliation":[{"name":"ProDigest BV , 9052 Zwijnaarde 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